Long-read sequencing reveals atypical mitochondrial genome structure in a New Zealand marine isopod
Most animal mitochondrial genomes are small, circular and structurally conserved. However, recent work indicates that diverse taxa possess unusual mitochondrial genomes. In Isopoda, species in multiple lineages have atypical and rearranged mitochondrial genomes. However, more species of this specios...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The Royal Society
2022-01-01
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| Series: | Royal Society Open Science |
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| Online Access: | https://royalsocietypublishing.org/doi/10.1098/rsos.211550 |
| _version_ | 1797837626600849408 |
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| author | William S. Pearman Sarah J. Wells James Dale Olin K. Silander Nikki E. Freed |
| author_facet | William S. Pearman Sarah J. Wells James Dale Olin K. Silander Nikki E. Freed |
| author_sort | William S. Pearman |
| collection | DOAJ |
| description | Most animal mitochondrial genomes are small, circular and structurally conserved. However, recent work indicates that diverse taxa possess unusual mitochondrial genomes. In Isopoda, species in multiple lineages have atypical and rearranged mitochondrial genomes. However, more species of this speciose taxon need to be evaluated to understand the evolutionary origins of atypical mitochondrial genomes in this group. In this study, we report the presence of an atypical mitochondrial structure in the New Zealand endemic marine isopod, Isocladus armatus. Data from long- and short-read DNA sequencing suggest that I. armatus has two mitochondrial chromosomes. The first chromosome consists of two mitochondrial genomes that have been inverted and fused together in a circular form, and the second chromosome consists of a single mitochondrial genome in a linearized form. This atypical mitochondrial structure has been detected in other isopod lineages, and our data from an additional divergent isopod lineage (Sphaeromatidae) lends support to the hypothesis that atypical structure evolved early in the evolution of Isopoda. Additionally, we find that an asymmetrical site previously observed across many species within Isopoda is absent in I. armatus, but confirm the presence of two asymmetrical sites recently reported in two other isopod species. |
| first_indexed | 2024-04-09T15:27:43Z |
| format | Article |
| id | doaj.art-35f375dcb27f4c449043ab84450bfa7e |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-04-09T15:27:43Z |
| publishDate | 2022-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-35f375dcb27f4c449043ab84450bfa7e2023-04-28T11:04:05ZengThe Royal SocietyRoyal Society Open Science2054-57032022-01-019110.1098/rsos.211550Long-read sequencing reveals atypical mitochondrial genome structure in a New Zealand marine isopodWilliam S. Pearman0Sarah J. Wells1James Dale2Olin K. Silander3Nikki E. Freed4School of Natural and Computational Sciences, Massey University-Albany Campus, Auckland, Auckland New ZealandSchool of Environmental and Animal Sciences, Unitec Institute of Technology, Auckland, New ZealandSchool of Natural and Computational Sciences, Massey University-Albany Campus, Auckland, Auckland New ZealandSchool of Natural and Computational Sciences, Massey University-Albany Campus, Auckland, Auckland New ZealandSchool of Natural and Computational Sciences, Massey University-Albany Campus, Auckland, Auckland New ZealandMost animal mitochondrial genomes are small, circular and structurally conserved. However, recent work indicates that diverse taxa possess unusual mitochondrial genomes. In Isopoda, species in multiple lineages have atypical and rearranged mitochondrial genomes. However, more species of this speciose taxon need to be evaluated to understand the evolutionary origins of atypical mitochondrial genomes in this group. In this study, we report the presence of an atypical mitochondrial structure in the New Zealand endemic marine isopod, Isocladus armatus. Data from long- and short-read DNA sequencing suggest that I. armatus has two mitochondrial chromosomes. The first chromosome consists of two mitochondrial genomes that have been inverted and fused together in a circular form, and the second chromosome consists of a single mitochondrial genome in a linearized form. This atypical mitochondrial structure has been detected in other isopod lineages, and our data from an additional divergent isopod lineage (Sphaeromatidae) lends support to the hypothesis that atypical structure evolved early in the evolution of Isopoda. Additionally, we find that an asymmetrical site previously observed across many species within Isopoda is absent in I. armatus, but confirm the presence of two asymmetrical sites recently reported in two other isopod species.https://royalsocietypublishing.org/doi/10.1098/rsos.211550mitochondriaheteroplasmypalindromicinverted repeatisopodasymmetry |
| spellingShingle | William S. Pearman Sarah J. Wells James Dale Olin K. Silander Nikki E. Freed Long-read sequencing reveals atypical mitochondrial genome structure in a New Zealand marine isopod Royal Society Open Science mitochondria heteroplasmy palindromic inverted repeat isopod asymmetry |
| title | Long-read sequencing reveals atypical mitochondrial genome structure in a New Zealand marine isopod |
| title_full | Long-read sequencing reveals atypical mitochondrial genome structure in a New Zealand marine isopod |
| title_fullStr | Long-read sequencing reveals atypical mitochondrial genome structure in a New Zealand marine isopod |
| title_full_unstemmed | Long-read sequencing reveals atypical mitochondrial genome structure in a New Zealand marine isopod |
| title_short | Long-read sequencing reveals atypical mitochondrial genome structure in a New Zealand marine isopod |
| title_sort | long read sequencing reveals atypical mitochondrial genome structure in a new zealand marine isopod |
| topic | mitochondria heteroplasmy palindromic inverted repeat isopod asymmetry |
| url | https://royalsocietypublishing.org/doi/10.1098/rsos.211550 |
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