Insights into the Bioinformatics and Transcriptional Analysis of the Elongator Complexes (<i>ELPs</i>) Gene Family of Wheat: <i>TaELPs</i> Contribute to Wheat Abiotic Stress Tolerance and Leaf Senescence
Elongator complexes (<i>ELPs</i>) are the protein complexes that promote transcription through histone acetylation in eukaryotic cells and interact with elongating RNA polymerase II (RNAPII). <i>ELPs</i>’ role in plant growth and development, signal transduction, and response...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/4/952 |
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| author | Feng Guo Md Ashraful Islam Chenxu Lv Xiujuan Jin Lili Sun Kai Zhao Juan Lu Rongyue Yan Wenjun Zhang Yugang Shi Ning Li Daizhen Sun |
| author_facet | Feng Guo Md Ashraful Islam Chenxu Lv Xiujuan Jin Lili Sun Kai Zhao Juan Lu Rongyue Yan Wenjun Zhang Yugang Shi Ning Li Daizhen Sun |
| author_sort | Feng Guo |
| collection | DOAJ |
| description | Elongator complexes (<i>ELPs</i>) are the protein complexes that promote transcription through histone acetylation in eukaryotic cells and interact with elongating RNA polymerase II (RNAPII). <i>ELPs</i>’ role in plant growth and development, signal transduction, and response to biotic and abiotic stresses have been confirmed in model plants. However, the functions of the wheat <i>ELP</i> genes are not well documented. The present study identified 18 members of the <i>ELPs</i> from the wheat genome with a homology search. Further, bioinformatics and transcription patterns in response to different stress conditions were analyzed to dissect their potential regulatory mechanisms in wheat. Gene duplication analysis showed that 18 pairs of <i>ELP</i> paralogous genes were derived from segmental duplication, which was divided into six clades by protein phylogenetic and cluster analysis. The orthologous analysis of wheat <i>TaELP</i> genes showed that <i>TaELP</i> genes may have evolved from orthologous genes of other plant species or closely related plants. Moreover, a variety of cis-acting regulatory elements (CAREs) related to growth and development, hormone response, and biotic and abiotic stresses were identified in the <i>TaELPs’</i> promoter regions. The qRT-PCR analysis showed that the transcription of <i>TaELPs</i> was induced under hormone, salt, and drought stress and during leaf senescence. The <i>TaELP2</i> gene was silenced with BSMV-VIGS, and <i>TaELP2</i> was preliminarily verified to be involved in the regulation of wheat leaf senescence. Overall, <i>TaELP</i> genes might be regulated by hormone signaling pathways and response to abiotic stress and leaf senescence, which could be investigated further as potential candidate genes for wheat abiotic stress tolerance and yield improvement. |
| first_indexed | 2024-03-11T08:14:36Z |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T08:14:36Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-35f48331cf61484b877a336575de07bc2023-11-16T22:49:40ZengMDPI AGPlants2223-77472023-02-0112495210.3390/plants12040952Insights into the Bioinformatics and Transcriptional Analysis of the Elongator Complexes (<i>ELPs</i>) Gene Family of Wheat: <i>TaELPs</i> Contribute to Wheat Abiotic Stress Tolerance and Leaf SenescenceFeng Guo0Md Ashraful Islam1Chenxu Lv2Xiujuan Jin3Lili Sun4Kai Zhao5Juan Lu6Rongyue Yan7Wenjun Zhang8Yugang Shi9Ning Li10Daizhen Sun11State Key Laboratory of Sustainable Dryland Agriculture, College of Agronomy, Shanxi Agricultural University, Taigu 030801, ChinaState Key Laboratory of Sustainable Dryland Agriculture, College of Agronomy, Shanxi Agricultural University, Taigu 030801, ChinaState Key Laboratory of Sustainable Dryland Agriculture, College of Agronomy, Shanxi Agricultural University, Taigu 030801, ChinaState Key Laboratory of Sustainable Dryland Agriculture, College of Agronomy, Shanxi Agricultural University, Taigu 030801, ChinaState Key Laboratory of Sustainable Dryland Agriculture, College of Agronomy, Shanxi Agricultural University, Taigu 030801, ChinaState Key Laboratory of Sustainable Dryland Agriculture, College of Agronomy, Shanxi Agricultural University, Taigu 030801, ChinaState Key Laboratory of Sustainable Dryland Agriculture, College of Agronomy, Shanxi Agricultural University, Taigu 030801, ChinaState Key Laboratory of Sustainable Dryland Agriculture, College of Agronomy, Shanxi Agricultural University, Taigu 030801, ChinaState Key Laboratory of Sustainable Dryland Agriculture, College of Agronomy, Shanxi Agricultural University, Taigu 030801, ChinaState Key Laboratory of Sustainable Dryland Agriculture, College of Agronomy, Shanxi Agricultural University, Taigu 030801, ChinaState Key Laboratory of Sustainable Dryland Agriculture, College of Agronomy, Shanxi Agricultural University, Taigu 030801, ChinaState Key Laboratory of Sustainable Dryland Agriculture, College of Agronomy, Shanxi Agricultural University, Taigu 030801, ChinaElongator complexes (<i>ELPs</i>) are the protein complexes that promote transcription through histone acetylation in eukaryotic cells and interact with elongating RNA polymerase II (RNAPII). <i>ELPs</i>’ role in plant growth and development, signal transduction, and response to biotic and abiotic stresses have been confirmed in model plants. However, the functions of the wheat <i>ELP</i> genes are not well documented. The present study identified 18 members of the <i>ELPs</i> from the wheat genome with a homology search. Further, bioinformatics and transcription patterns in response to different stress conditions were analyzed to dissect their potential regulatory mechanisms in wheat. Gene duplication analysis showed that 18 pairs of <i>ELP</i> paralogous genes were derived from segmental duplication, which was divided into six clades by protein phylogenetic and cluster analysis. The orthologous analysis of wheat <i>TaELP</i> genes showed that <i>TaELP</i> genes may have evolved from orthologous genes of other plant species or closely related plants. Moreover, a variety of cis-acting regulatory elements (CAREs) related to growth and development, hormone response, and biotic and abiotic stresses were identified in the <i>TaELPs’</i> promoter regions. The qRT-PCR analysis showed that the transcription of <i>TaELPs</i> was induced under hormone, salt, and drought stress and during leaf senescence. The <i>TaELP2</i> gene was silenced with BSMV-VIGS, and <i>TaELP2</i> was preliminarily verified to be involved in the regulation of wheat leaf senescence. Overall, <i>TaELP</i> genes might be regulated by hormone signaling pathways and response to abiotic stress and leaf senescence, which could be investigated further as potential candidate genes for wheat abiotic stress tolerance and yield improvement.https://www.mdpi.com/2223-7747/12/4/952Elongator complexes (<i>ELPs</i>)in silico analysisabiotic stressleaf senescencewheat |
| spellingShingle | Feng Guo Md Ashraful Islam Chenxu Lv Xiujuan Jin Lili Sun Kai Zhao Juan Lu Rongyue Yan Wenjun Zhang Yugang Shi Ning Li Daizhen Sun Insights into the Bioinformatics and Transcriptional Analysis of the Elongator Complexes (<i>ELPs</i>) Gene Family of Wheat: <i>TaELPs</i> Contribute to Wheat Abiotic Stress Tolerance and Leaf Senescence Plants Elongator complexes (<i>ELPs</i>) in silico analysis abiotic stress leaf senescence wheat |
| title | Insights into the Bioinformatics and Transcriptional Analysis of the Elongator Complexes (<i>ELPs</i>) Gene Family of Wheat: <i>TaELPs</i> Contribute to Wheat Abiotic Stress Tolerance and Leaf Senescence |
| title_full | Insights into the Bioinformatics and Transcriptional Analysis of the Elongator Complexes (<i>ELPs</i>) Gene Family of Wheat: <i>TaELPs</i> Contribute to Wheat Abiotic Stress Tolerance and Leaf Senescence |
| title_fullStr | Insights into the Bioinformatics and Transcriptional Analysis of the Elongator Complexes (<i>ELPs</i>) Gene Family of Wheat: <i>TaELPs</i> Contribute to Wheat Abiotic Stress Tolerance and Leaf Senescence |
| title_full_unstemmed | Insights into the Bioinformatics and Transcriptional Analysis of the Elongator Complexes (<i>ELPs</i>) Gene Family of Wheat: <i>TaELPs</i> Contribute to Wheat Abiotic Stress Tolerance and Leaf Senescence |
| title_short | Insights into the Bioinformatics and Transcriptional Analysis of the Elongator Complexes (<i>ELPs</i>) Gene Family of Wheat: <i>TaELPs</i> Contribute to Wheat Abiotic Stress Tolerance and Leaf Senescence |
| title_sort | insights into the bioinformatics and transcriptional analysis of the elongator complexes i elps i gene family of wheat i taelps i contribute to wheat abiotic stress tolerance and leaf senescence |
| topic | Elongator complexes (<i>ELPs</i>) in silico analysis abiotic stress leaf senescence wheat |
| url | https://www.mdpi.com/2223-7747/12/4/952 |
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