Genome-Wide Transcriptome Analysis Revealing the Genes Related to Sugar Metabolism in Kernels of Sweet Corn
Sugar metabolism influences the quality of sweet corn (<i>Zea mays</i> var. <i>saccharate</i> Sturt) kernels, which is a major goal for maize breeding. In this study, the genome-wide transcriptomes from two supersweet corn cultivars (cv. Xuetian 7401 and Zhetian 11) with a ne...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/12/1254 |
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| author | Bin Chen Shouli Feng Junfeng Hou Ying Zhu Fei Bao Hailiang Han Heping Tan Guiyue Wang Fucheng Zhao |
| author_facet | Bin Chen Shouli Feng Junfeng Hou Ying Zhu Fei Bao Hailiang Han Heping Tan Guiyue Wang Fucheng Zhao |
| author_sort | Bin Chen |
| collection | DOAJ |
| description | Sugar metabolism influences the quality of sweet corn (<i>Zea mays</i> var. <i>saccharate</i> Sturt) kernels, which is a major goal for maize breeding. In this study, the genome-wide transcriptomes from two supersweet corn cultivars (cv. Xuetian 7401 and Zhetian 11) with a nearly two-fold difference in kernel sugar content were carried out to explore the genes related to kernel sugar metabolism. In total, 45,748 differentially expressed genes (DEGs) in kernels and 596 DEGs in leaves were identified. PsbS, photosynthetic system II subunit S, showed two isoforms with different expression levels in leaf tissue between two cultivars, indicating that this gene might influence sugar accumulation in the kernel. On the other hand, hexokinases and beta-glucosidase genes involved in glycolysis, starch and sucrose metabolism were found in developing kernels with a genome-wide transcriptome analysis of developing kernels, which might contribute to the overaccumulation of water-soluble polysaccharides and an increase in the sweetness in the kernels of Xuetian 7401. These results indicated that kernel sugar accumulation in sweet corn might be influenced by both photosynthesis efficiency and the sugar metabolism rate. Our study supplied a new insight for breeding new cultivars with high sugar content and laid the foundation for exploring the regulatory mechanisms of kernel sugar content in corn. |
| first_indexed | 2024-03-09T16:05:56Z |
| format | Article |
| id | doaj.art-35f5a57c83e444498c15cf99646c186f |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T16:05:56Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-35f5a57c83e444498c15cf99646c186f2023-11-24T16:38:21ZengMDPI AGMetabolites2218-19892022-12-011212125410.3390/metabo12121254Genome-Wide Transcriptome Analysis Revealing the Genes Related to Sugar Metabolism in Kernels of Sweet CornBin Chen0Shouli Feng1Junfeng Hou2Ying Zhu3Fei Bao4Hailiang Han5Heping Tan6Guiyue Wang7Fucheng Zhao8Institute of Maize and Featured Dryland Crops, Zhejiang Academy of Agriculture Sciences, Dongyang 322100, ChinaCollege of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310030, ChinaInstitute of Maize and Featured Dryland Crops, Zhejiang Academy of Agriculture Sciences, Dongyang 322100, ChinaInstitute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310022, ChinaInstitute of Maize and Featured Dryland Crops, Zhejiang Academy of Agriculture Sciences, Dongyang 322100, ChinaInstitute of Maize and Featured Dryland Crops, Zhejiang Academy of Agriculture Sciences, Dongyang 322100, ChinaInstitute of Maize and Featured Dryland Crops, Zhejiang Academy of Agriculture Sciences, Dongyang 322100, ChinaInstitute of Maize and Featured Dryland Crops, Zhejiang Academy of Agriculture Sciences, Dongyang 322100, ChinaInstitute of Maize and Featured Dryland Crops, Zhejiang Academy of Agriculture Sciences, Dongyang 322100, ChinaSugar metabolism influences the quality of sweet corn (<i>Zea mays</i> var. <i>saccharate</i> Sturt) kernels, which is a major goal for maize breeding. In this study, the genome-wide transcriptomes from two supersweet corn cultivars (cv. Xuetian 7401 and Zhetian 11) with a nearly two-fold difference in kernel sugar content were carried out to explore the genes related to kernel sugar metabolism. In total, 45,748 differentially expressed genes (DEGs) in kernels and 596 DEGs in leaves were identified. PsbS, photosynthetic system II subunit S, showed two isoforms with different expression levels in leaf tissue between two cultivars, indicating that this gene might influence sugar accumulation in the kernel. On the other hand, hexokinases and beta-glucosidase genes involved in glycolysis, starch and sucrose metabolism were found in developing kernels with a genome-wide transcriptome analysis of developing kernels, which might contribute to the overaccumulation of water-soluble polysaccharides and an increase in the sweetness in the kernels of Xuetian 7401. These results indicated that kernel sugar accumulation in sweet corn might be influenced by both photosynthesis efficiency and the sugar metabolism rate. Our study supplied a new insight for breeding new cultivars with high sugar content and laid the foundation for exploring the regulatory mechanisms of kernel sugar content in corn.https://www.mdpi.com/2218-1989/12/12/1254sweet corn (<i>Zea mays </i>var. <i>saccharate </i>Sturt)kernel sugar metabolismgenome-wide transcriptome analysis |
| spellingShingle | Bin Chen Shouli Feng Junfeng Hou Ying Zhu Fei Bao Hailiang Han Heping Tan Guiyue Wang Fucheng Zhao Genome-Wide Transcriptome Analysis Revealing the Genes Related to Sugar Metabolism in Kernels of Sweet Corn Metabolites sweet corn (<i>Zea mays </i>var. <i>saccharate </i>Sturt) kernel sugar metabolism genome-wide transcriptome analysis |
| title | Genome-Wide Transcriptome Analysis Revealing the Genes Related to Sugar Metabolism in Kernels of Sweet Corn |
| title_full | Genome-Wide Transcriptome Analysis Revealing the Genes Related to Sugar Metabolism in Kernels of Sweet Corn |
| title_fullStr | Genome-Wide Transcriptome Analysis Revealing the Genes Related to Sugar Metabolism in Kernels of Sweet Corn |
| title_full_unstemmed | Genome-Wide Transcriptome Analysis Revealing the Genes Related to Sugar Metabolism in Kernels of Sweet Corn |
| title_short | Genome-Wide Transcriptome Analysis Revealing the Genes Related to Sugar Metabolism in Kernels of Sweet Corn |
| title_sort | genome wide transcriptome analysis revealing the genes related to sugar metabolism in kernels of sweet corn |
| topic | sweet corn (<i>Zea mays </i>var. <i>saccharate </i>Sturt) kernel sugar metabolism genome-wide transcriptome analysis |
| url | https://www.mdpi.com/2218-1989/12/12/1254 |
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