Involvement of Polyamines From Cardiac Mast Cells in Myocardial Remodeling Induced by Pressure Overload Through Mitochondrial Permeability Transition Pore Opening
ObjectivePolyamines mainly contain spermine (SPM), spermidine (SPD), and putrescine (PUT). Many research results suggest that polyamines participate in cell proliferation, differentiation, and the regulation of gene expression, and have a close relationship with the occurrence and development of man...
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| Format: | Article |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Cardiovascular Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcvm.2022.850688/full |
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| author | Xiaolan Xiong Xiaolan Xiong Xiaolan Xiong Junming Li Shizhong Zhang Shizhong Zhang Xiaoli Jia Xiaoli Jia Chao Xiao Chao Xiao |
| author_facet | Xiaolan Xiong Xiaolan Xiong Xiaolan Xiong Junming Li Shizhong Zhang Shizhong Zhang Xiaoli Jia Xiaoli Jia Chao Xiao Chao Xiao |
| author_sort | Xiaolan Xiong |
| collection | DOAJ |
| description | ObjectivePolyamines mainly contain spermine (SPM), spermidine (SPD), and putrescine (PUT). Many research results suggest that polyamines participate in cell proliferation, differentiation, and the regulation of gene expression, and have a close relationship with the occurrence and development of many diseases. However, the role and possible mechanisms of action of polyamines from cardiac mast cells in myocardial remodeling induced by pressure overload remain to be elucidated.MethodsPressure overload was induced by abdominal aortic constriction (AAC). Toluidine blue staining was used to visualize mast cells in cardiac tissue. The polyamine content of cardiac tissue was analyzed using high-performance liquid chromatography. Opening of the mitochondrial permeability transition pore (MPTP) was determined by the Ca2+-induced swelling of isolated cardiac mitochondria, measured as a reduction in A520.ResultsCompared with sham rats, the cardiac mast cell density, the polyamine content (PUT, SPB, and SPM), and myocardial MPTP opening in rats with AAC were significantly increased (P < 0.05), and were accompanied by increased myocardial fibrosis and heart weight/body weight ratio. Intraperitoneal injection of polyamines mimicked these results, and these effects were reversed by cromolyn sodium, a mast cell stabilizer (P < 0.05). Myocardial MPTP opening increased in rats with AAC (P < 0.05), and the three polyamines also increased myocardial MPTP opening (P < 0.05).ConclusionMast cell-derived polyamines are involved in pressure overload-induced myocardial remodeling by increasing opening of the MPTP. |
| first_indexed | 2024-04-14T02:41:04Z |
| format | Article |
| id | doaj.art-35f617a43de742b890205266f8efffd3 |
| institution | Directory Open Access Journal |
| issn | 2297-055X |
| language | English |
| last_indexed | 2024-04-14T02:41:04Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cardiovascular Medicine |
| spelling | doaj.art-35f617a43de742b890205266f8efffd32022-12-22T02:17:02ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2022-04-01910.3389/fcvm.2022.850688850688Involvement of Polyamines From Cardiac Mast Cells in Myocardial Remodeling Induced by Pressure Overload Through Mitochondrial Permeability Transition Pore OpeningXiaolan Xiong0Xiaolan Xiong1Xiaolan Xiong2Junming Li3Shizhong Zhang4Shizhong Zhang5Xiaoli Jia6Xiaoli Jia7Chao Xiao8Chao Xiao9Third-Grade Pharmacological Laboratory on Traditional Chinese Medicine China Three Gorges University, Yichang, ChinaMedical College, China Three Gorges University, Yichang, ChinaThe Second People’s Hospital of Yichang, Yichang, ChinaThe First People’s Hospital of Yichang, Yichang, ChinaThird-Grade Pharmacological Laboratory on Traditional Chinese Medicine China Three Gorges University, Yichang, ChinaMedical College, China Three Gorges University, Yichang, ChinaThird-Grade Pharmacological Laboratory on Traditional Chinese Medicine China Three Gorges University, Yichang, ChinaMedical College, China Three Gorges University, Yichang, ChinaThird-Grade Pharmacological Laboratory on Traditional Chinese Medicine China Three Gorges University, Yichang, ChinaMedical College, China Three Gorges University, Yichang, ChinaObjectivePolyamines mainly contain spermine (SPM), spermidine (SPD), and putrescine (PUT). Many research results suggest that polyamines participate in cell proliferation, differentiation, and the regulation of gene expression, and have a close relationship with the occurrence and development of many diseases. However, the role and possible mechanisms of action of polyamines from cardiac mast cells in myocardial remodeling induced by pressure overload remain to be elucidated.MethodsPressure overload was induced by abdominal aortic constriction (AAC). Toluidine blue staining was used to visualize mast cells in cardiac tissue. The polyamine content of cardiac tissue was analyzed using high-performance liquid chromatography. Opening of the mitochondrial permeability transition pore (MPTP) was determined by the Ca2+-induced swelling of isolated cardiac mitochondria, measured as a reduction in A520.ResultsCompared with sham rats, the cardiac mast cell density, the polyamine content (PUT, SPB, and SPM), and myocardial MPTP opening in rats with AAC were significantly increased (P < 0.05), and were accompanied by increased myocardial fibrosis and heart weight/body weight ratio. Intraperitoneal injection of polyamines mimicked these results, and these effects were reversed by cromolyn sodium, a mast cell stabilizer (P < 0.05). Myocardial MPTP opening increased in rats with AAC (P < 0.05), and the three polyamines also increased myocardial MPTP opening (P < 0.05).ConclusionMast cell-derived polyamines are involved in pressure overload-induced myocardial remodeling by increasing opening of the MPTP.https://www.frontiersin.org/articles/10.3389/fcvm.2022.850688/fullpressure overloadcardiac remodelingmast cellpolyamineMPTP |
| spellingShingle | Xiaolan Xiong Xiaolan Xiong Xiaolan Xiong Junming Li Shizhong Zhang Shizhong Zhang Xiaoli Jia Xiaoli Jia Chao Xiao Chao Xiao Involvement of Polyamines From Cardiac Mast Cells in Myocardial Remodeling Induced by Pressure Overload Through Mitochondrial Permeability Transition Pore Opening Frontiers in Cardiovascular Medicine pressure overload cardiac remodeling mast cell polyamine MPTP |
| title | Involvement of Polyamines From Cardiac Mast Cells in Myocardial Remodeling Induced by Pressure Overload Through Mitochondrial Permeability Transition Pore Opening |
| title_full | Involvement of Polyamines From Cardiac Mast Cells in Myocardial Remodeling Induced by Pressure Overload Through Mitochondrial Permeability Transition Pore Opening |
| title_fullStr | Involvement of Polyamines From Cardiac Mast Cells in Myocardial Remodeling Induced by Pressure Overload Through Mitochondrial Permeability Transition Pore Opening |
| title_full_unstemmed | Involvement of Polyamines From Cardiac Mast Cells in Myocardial Remodeling Induced by Pressure Overload Through Mitochondrial Permeability Transition Pore Opening |
| title_short | Involvement of Polyamines From Cardiac Mast Cells in Myocardial Remodeling Induced by Pressure Overload Through Mitochondrial Permeability Transition Pore Opening |
| title_sort | involvement of polyamines from cardiac mast cells in myocardial remodeling induced by pressure overload through mitochondrial permeability transition pore opening |
| topic | pressure overload cardiac remodeling mast cell polyamine MPTP |
| url | https://www.frontiersin.org/articles/10.3389/fcvm.2022.850688/full |
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