Transcriptomic immune response of Tenebrio molitor pupae to parasitization by Scleroderma guani.
BACKGROUND: Host and parasitoid interaction is one of the most fascinating relationships of insects, which is currently receiving an increasing interest. Understanding the mechanisms evolved by the parasitoids to evade or suppress the host immune system is important for dissecting this interaction,...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3544796?pdf=render |
| _version_ | 1811207899443101696 |
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| author | Jia-Ying Zhu Pu Yang Zhong Zhang Guo-Xing Wu Bin Yang |
| author_facet | Jia-Ying Zhu Pu Yang Zhong Zhang Guo-Xing Wu Bin Yang |
| author_sort | Jia-Ying Zhu |
| collection | DOAJ |
| description | BACKGROUND: Host and parasitoid interaction is one of the most fascinating relationships of insects, which is currently receiving an increasing interest. Understanding the mechanisms evolved by the parasitoids to evade or suppress the host immune system is important for dissecting this interaction, while it was still poorly known. In order to gain insight into the immune response of Tenebrio molitor to parasitization by Scleroderma guani, the transcriptome of T. molitor pupae was sequenced with focus on immune-related gene, and the non-parasitized and parasitized T. molitor pupae were analyzed by digital gene expression (DGE) analysis with special emphasis on parasitoid-induced immune-related genes using Illumina sequencing. METHODOLOGY/PRINCIPAL FINDINGS: In a single run, 264,698 raw reads were obtained. De novo assembly generated 71,514 unigenes with mean length of 424 bp. Of those unigenes, 37,373 (52.26%) showed similarity to the known proteins in the NCBI nr database. Via analysis of the transcriptome data in depth, 430 unigenes related to immunity were identified. DGE analysis revealed that parasitization by S. guani had considerable impacts on the transcriptome profile of T. molitor pupae, as indicated by the significant up- or down-regulation of 3,431 parasitism-responsive transcripts. The expression of a total of 74 unigenes involved in immune response of T. molitor was significantly altered after parasitization. CONCLUSIONS/SIGNIFICANCE: obtained T. molitor transcriptome, in addition to establishing a fundamental resource for further research on functional genomics, has allowed the discovery of a large group of immune genes that might provide a meaningful framework to better understand the immune response in this species and other beetles. The DGE profiling data provides comprehensive T. molitor immune gene expression information at the transcriptional level following parasitization, and sheds valuable light on the molecular understanding of the host-parasitoid interaction. |
| first_indexed | 2024-04-12T04:12:45Z |
| format | Article |
| id | doaj.art-35f7e30c406441c194388df7970d356b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T04:12:45Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-35f7e30c406441c194388df7970d356b2022-12-22T03:48:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0181e5441110.1371/journal.pone.0054411Transcriptomic immune response of Tenebrio molitor pupae to parasitization by Scleroderma guani.Jia-Ying ZhuPu YangZhong ZhangGuo-Xing WuBin YangBACKGROUND: Host and parasitoid interaction is one of the most fascinating relationships of insects, which is currently receiving an increasing interest. Understanding the mechanisms evolved by the parasitoids to evade or suppress the host immune system is important for dissecting this interaction, while it was still poorly known. In order to gain insight into the immune response of Tenebrio molitor to parasitization by Scleroderma guani, the transcriptome of T. molitor pupae was sequenced with focus on immune-related gene, and the non-parasitized and parasitized T. molitor pupae were analyzed by digital gene expression (DGE) analysis with special emphasis on parasitoid-induced immune-related genes using Illumina sequencing. METHODOLOGY/PRINCIPAL FINDINGS: In a single run, 264,698 raw reads were obtained. De novo assembly generated 71,514 unigenes with mean length of 424 bp. Of those unigenes, 37,373 (52.26%) showed similarity to the known proteins in the NCBI nr database. Via analysis of the transcriptome data in depth, 430 unigenes related to immunity were identified. DGE analysis revealed that parasitization by S. guani had considerable impacts on the transcriptome profile of T. molitor pupae, as indicated by the significant up- or down-regulation of 3,431 parasitism-responsive transcripts. The expression of a total of 74 unigenes involved in immune response of T. molitor was significantly altered after parasitization. CONCLUSIONS/SIGNIFICANCE: obtained T. molitor transcriptome, in addition to establishing a fundamental resource for further research on functional genomics, has allowed the discovery of a large group of immune genes that might provide a meaningful framework to better understand the immune response in this species and other beetles. The DGE profiling data provides comprehensive T. molitor immune gene expression information at the transcriptional level following parasitization, and sheds valuable light on the molecular understanding of the host-parasitoid interaction.http://europepmc.org/articles/PMC3544796?pdf=render |
| spellingShingle | Jia-Ying Zhu Pu Yang Zhong Zhang Guo-Xing Wu Bin Yang Transcriptomic immune response of Tenebrio molitor pupae to parasitization by Scleroderma guani. PLoS ONE |
| title | Transcriptomic immune response of Tenebrio molitor pupae to parasitization by Scleroderma guani. |
| title_full | Transcriptomic immune response of Tenebrio molitor pupae to parasitization by Scleroderma guani. |
| title_fullStr | Transcriptomic immune response of Tenebrio molitor pupae to parasitization by Scleroderma guani. |
| title_full_unstemmed | Transcriptomic immune response of Tenebrio molitor pupae to parasitization by Scleroderma guani. |
| title_short | Transcriptomic immune response of Tenebrio molitor pupae to parasitization by Scleroderma guani. |
| title_sort | transcriptomic immune response of tenebrio molitor pupae to parasitization by scleroderma guani |
| url | http://europepmc.org/articles/PMC3544796?pdf=render |
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