Characterization of fetal microchimeric immune cells in mouse maternal hearts during physiologic and pathologic pregnancies
Introduction: During pregnancy, fetal cells can be incorporated into maternal tissues (fetal microchimerism), where they can persist postpartum. Whether these fetal cells are beneficial or detrimental to maternal health is unknown. This study aimed to characterize fetal microchimeric immune cells in...
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| Format: | Article |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2023.1256945/full |
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| author | Ryan C. V. Lintao Ryan C. V. Lintao Ananth Kumar Kammala Enkhtuya Radnaa Mohamed Bettayeb Kathleen L. Vincent Kathleen L. Vincent Igor Patrikeev Jerome Yaklic Elizabeth A. Bonney Ramkumar Menon |
| author_facet | Ryan C. V. Lintao Ryan C. V. Lintao Ananth Kumar Kammala Enkhtuya Radnaa Mohamed Bettayeb Kathleen L. Vincent Kathleen L. Vincent Igor Patrikeev Jerome Yaklic Elizabeth A. Bonney Ramkumar Menon |
| author_sort | Ryan C. V. Lintao |
| collection | DOAJ |
| description | Introduction: During pregnancy, fetal cells can be incorporated into maternal tissues (fetal microchimerism), where they can persist postpartum. Whether these fetal cells are beneficial or detrimental to maternal health is unknown. This study aimed to characterize fetal microchimeric immune cells in the maternal heart during pregnancy and postpartum, and to identify differences in these fetal microchimeric subpopulations between normal and pregnancies complicated by spontaneous preterm induced by ascending infection.Methods: A Cre reporter mouse model, which when mated with wild-type C57BL/6J females resulted in cells and tissues of progeny expressing red fluorescent protein tandem dimer Tomato (mT+), was used to detect fetal microchimeric cells. On embryonic day (E)15, 104 colony-forming units (CFU) E. coli was administered intravaginally to mimic ascending infection, with delivery on or before E18.5 considered as preterm delivery. A subset of pregnant mice was sacrificed at E16 and postpartum day 28 to harvest maternal hearts. Heart tissues were processed for immunofluorescence microscopy and high-dimensional mass cytometry by time-of-flight (CyTOF) using an antibody panel of immune cell markers. Changes in cardiac physiologic parameters were measured up to 60 days postpartum via two-dimensional echocardiography.Results: Intravaginal E. coli administration resulted in preterm delivery of live pups in 70% of the cases. mT + expressing cells were detected in maternal uterus and heart, implying that fetal cells can migrate to different maternal compartments. During ascending infection, more fetal antigen-presenting cells (APCs) and less fetal hematopoietic stem cells (HSCs) and fetal double-positive (DP) thymocytes were observed in maternal hearts at E16 compared to normal pregnancy. These HSCs were cleared while DP thymocytes persisted 28 days postpartum following an ascending infection. No significant changes in cardiac physiologic parameters were observed postpartum except a trend in lowering the ejection fraction rate in preterm delivered mothers.Conclusion: Both normal pregnancy and ascending infection revealed distinct compositions of fetal microchimeric immune cells within the maternal heart, which could potentially influence the maternal cardiac microenvironment via (1) modulation of cardiac reverse modeling processes by fetal stem cells, and (2) differential responses to recognition of fetal APCs by maternal T cells. |
| first_indexed | 2024-03-11T22:38:36Z |
| format | Article |
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| language | English |
| last_indexed | 2024-03-11T22:38:36Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-35f8418a963c41b58af6e42943fa08542023-09-22T09:49:00ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2023-09-011110.3389/fcell.2023.12569451256945Characterization of fetal microchimeric immune cells in mouse maternal hearts during physiologic and pathologic pregnanciesRyan C. V. Lintao0Ryan C. V. Lintao1Ananth Kumar Kammala2Enkhtuya Radnaa3Mohamed Bettayeb4Kathleen L. Vincent5Kathleen L. Vincent6Igor Patrikeev7Jerome Yaklic8Elizabeth A. Bonney9Ramkumar Menon10Division of Basic Science and Translational Research, Department of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, Galveston, TX, United StatesDepartment of Biochemistry and Molecular Biology, College of Medicine, University of the Philippines Manila, Manila, PhilippinesDivision of Basic Science and Translational Research, Department of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, Galveston, TX, United StatesDivision of Basic Science and Translational Research, Department of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, Galveston, TX, United StatesDivision of Basic Science and Translational Research, Department of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, Galveston, TX, United StatesDivision of Basic Science and Translational Research, Department of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, Galveston, TX, United StatesBiomedical Engineering and Imaging Sciences Group, The University of Texas Medical Branch at Galveston, Galveston, TX, United StatesBiomedical Engineering and Imaging Sciences Group, The University of Texas Medical Branch at Galveston, Galveston, TX, United StatesDepartment of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, Galveston, TX, United StatesDepartment of Obstetrics, Gynecology, and Reproductive Sciences, Larner College of Medicine, The University of Vermont, Burlington, VT, United StatesDivision of Basic Science and Translational Research, Department of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, Galveston, TX, United StatesIntroduction: During pregnancy, fetal cells can be incorporated into maternal tissues (fetal microchimerism), where they can persist postpartum. Whether these fetal cells are beneficial or detrimental to maternal health is unknown. This study aimed to characterize fetal microchimeric immune cells in the maternal heart during pregnancy and postpartum, and to identify differences in these fetal microchimeric subpopulations between normal and pregnancies complicated by spontaneous preterm induced by ascending infection.Methods: A Cre reporter mouse model, which when mated with wild-type C57BL/6J females resulted in cells and tissues of progeny expressing red fluorescent protein tandem dimer Tomato (mT+), was used to detect fetal microchimeric cells. On embryonic day (E)15, 104 colony-forming units (CFU) E. coli was administered intravaginally to mimic ascending infection, with delivery on or before E18.5 considered as preterm delivery. A subset of pregnant mice was sacrificed at E16 and postpartum day 28 to harvest maternal hearts. Heart tissues were processed for immunofluorescence microscopy and high-dimensional mass cytometry by time-of-flight (CyTOF) using an antibody panel of immune cell markers. Changes in cardiac physiologic parameters were measured up to 60 days postpartum via two-dimensional echocardiography.Results: Intravaginal E. coli administration resulted in preterm delivery of live pups in 70% of the cases. mT + expressing cells were detected in maternal uterus and heart, implying that fetal cells can migrate to different maternal compartments. During ascending infection, more fetal antigen-presenting cells (APCs) and less fetal hematopoietic stem cells (HSCs) and fetal double-positive (DP) thymocytes were observed in maternal hearts at E16 compared to normal pregnancy. These HSCs were cleared while DP thymocytes persisted 28 days postpartum following an ascending infection. No significant changes in cardiac physiologic parameters were observed postpartum except a trend in lowering the ejection fraction rate in preterm delivered mothers.Conclusion: Both normal pregnancy and ascending infection revealed distinct compositions of fetal microchimeric immune cells within the maternal heart, which could potentially influence the maternal cardiac microenvironment via (1) modulation of cardiac reverse modeling processes by fetal stem cells, and (2) differential responses to recognition of fetal APCs by maternal T cells.https://www.frontiersin.org/articles/10.3389/fcell.2023.1256945/fullmicrochimerismfetal microchimerismpreterm birthcardiovascular riskinfectionfetal cells |
| spellingShingle | Ryan C. V. Lintao Ryan C. V. Lintao Ananth Kumar Kammala Enkhtuya Radnaa Mohamed Bettayeb Kathleen L. Vincent Kathleen L. Vincent Igor Patrikeev Jerome Yaklic Elizabeth A. Bonney Ramkumar Menon Characterization of fetal microchimeric immune cells in mouse maternal hearts during physiologic and pathologic pregnancies Frontiers in Cell and Developmental Biology microchimerism fetal microchimerism preterm birth cardiovascular risk infection fetal cells |
| title | Characterization of fetal microchimeric immune cells in mouse maternal hearts during physiologic and pathologic pregnancies |
| title_full | Characterization of fetal microchimeric immune cells in mouse maternal hearts during physiologic and pathologic pregnancies |
| title_fullStr | Characterization of fetal microchimeric immune cells in mouse maternal hearts during physiologic and pathologic pregnancies |
| title_full_unstemmed | Characterization of fetal microchimeric immune cells in mouse maternal hearts during physiologic and pathologic pregnancies |
| title_short | Characterization of fetal microchimeric immune cells in mouse maternal hearts during physiologic and pathologic pregnancies |
| title_sort | characterization of fetal microchimeric immune cells in mouse maternal hearts during physiologic and pathologic pregnancies |
| topic | microchimerism fetal microchimerism preterm birth cardiovascular risk infection fetal cells |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2023.1256945/full |
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