Photocrosslinkable Artificial Nucleic Acid Probe Based miRNA Biosensor
Molecular recognition elements like enzymes, antibodies, and nucleic acids, which are involved in specific binding, are important components in biosensing technologies. These biomolecular recognition elements are based on molecular interactions such as hydrogen bonding, van der Waals forces, and hyd...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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The Electrochemical Society of Japan
2024-02-01
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| Series: | Electrochemistry |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/electrochemistry/92/2/92_23-68119/_html/-char/en |
| _version_ | 1797291953464803328 |
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| author | Yui WATANABE Shuto YAJIMA Maho KODA Ayako KOTO Eiichiro TAKAMURA Hiroaki SAKAMOTO |
| author_facet | Yui WATANABE Shuto YAJIMA Maho KODA Ayako KOTO Eiichiro TAKAMURA Hiroaki SAKAMOTO |
| author_sort | Yui WATANABE |
| collection | DOAJ |
| description | Molecular recognition elements like enzymes, antibodies, and nucleic acids, which are involved in specific binding, are important components in biosensing technologies. These biomolecular recognition elements are based on molecular interactions such as hydrogen bonding, van der Waals forces, and hydrophobic interactions. However, these interactions are often affected by the solution environment such as pH, temperature, and salt concentration, which are the rate-limiting factors for biosensing applications. In this study, we focused on molecular recognition using photocrosslinkable artificial nucleic acids. Photocrosslinkable artificial nucleic acids can form covalent bonds with target nucleic acids upon photoirradiation after hybridization. The covalent bonds formed are stronger than those in conventional molecular recognition and are not affected by the solution environment. Herein, we propose a biosensing system that combines molecular recognition by photocrosslinkable artificial nucleic acids, isothermal amplification by hybridization chain reaction, and electrochemical detection of miR-21 as the target molecule, which has recently attracted attention as a cancer biomarker. This technology eliminates non-specific binding and enables biosensing measurements with a suppressed background. |
| first_indexed | 2024-03-07T19:45:12Z |
| format | Article |
| id | doaj.art-35fa1776f8484734900b2d5abfb83e1a |
| institution | Directory Open Access Journal |
| issn | 2186-2451 |
| language | English |
| last_indexed | 2024-03-07T19:45:12Z |
| publishDate | 2024-02-01 |
| publisher | The Electrochemical Society of Japan |
| record_format | Article |
| series | Electrochemistry |
| spelling | doaj.art-35fa1776f8484734900b2d5abfb83e1a2024-02-29T02:10:51ZengThe Electrochemical Society of JapanElectrochemistry2186-24512024-02-0192202201802201810.5796/electrochemistry.23-68119electrochemistryPhotocrosslinkable Artificial Nucleic Acid Probe Based miRNA BiosensorYui WATANABE0Shuto YAJIMA1Maho KODA2Ayako KOTO3Eiichiro TAKAMURA4Hiroaki SAKAMOTO5https://orcid.org/0000-0002-6300-4055Department of Frontier Fiber and Technology and Science, Graduate School of Engineering, University of FukuiDepartment of Frontier Fiber and Technology and Science, Graduate School of Engineering, University of FukuiDepartment of Frontier Fiber and Technology and Science, Graduate School of Engineering, University of FukuiNicca Chemical Co., Ltd.Department of Frontier Fiber and Technology and Science, Graduate School of Engineering, University of FukuiDepartment of Frontier Fiber and Technology and Science, Graduate School of Engineering, University of FukuiMolecular recognition elements like enzymes, antibodies, and nucleic acids, which are involved in specific binding, are important components in biosensing technologies. These biomolecular recognition elements are based on molecular interactions such as hydrogen bonding, van der Waals forces, and hydrophobic interactions. However, these interactions are often affected by the solution environment such as pH, temperature, and salt concentration, which are the rate-limiting factors for biosensing applications. In this study, we focused on molecular recognition using photocrosslinkable artificial nucleic acids. Photocrosslinkable artificial nucleic acids can form covalent bonds with target nucleic acids upon photoirradiation after hybridization. The covalent bonds formed are stronger than those in conventional molecular recognition and are not affected by the solution environment. Herein, we propose a biosensing system that combines molecular recognition by photocrosslinkable artificial nucleic acids, isothermal amplification by hybridization chain reaction, and electrochemical detection of miR-21 as the target molecule, which has recently attracted attention as a cancer biomarker. This technology eliminates non-specific binding and enables biosensing measurements with a suppressed background.https://www.jstage.jst.go.jp/article/electrochemistry/92/2/92_23-68119/_html/-char/enartificial nucleic acidmolecular recognitionbiosensingmirna |
| spellingShingle | Yui WATANABE Shuto YAJIMA Maho KODA Ayako KOTO Eiichiro TAKAMURA Hiroaki SAKAMOTO Photocrosslinkable Artificial Nucleic Acid Probe Based miRNA Biosensor Electrochemistry artificial nucleic acid molecular recognition biosensing mirna |
| title | Photocrosslinkable Artificial Nucleic Acid Probe Based miRNA Biosensor |
| title_full | Photocrosslinkable Artificial Nucleic Acid Probe Based miRNA Biosensor |
| title_fullStr | Photocrosslinkable Artificial Nucleic Acid Probe Based miRNA Biosensor |
| title_full_unstemmed | Photocrosslinkable Artificial Nucleic Acid Probe Based miRNA Biosensor |
| title_short | Photocrosslinkable Artificial Nucleic Acid Probe Based miRNA Biosensor |
| title_sort | photocrosslinkable artificial nucleic acid probe based mirna biosensor |
| topic | artificial nucleic acid molecular recognition biosensing mirna |
| url | https://www.jstage.jst.go.jp/article/electrochemistry/92/2/92_23-68119/_html/-char/en |
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