circNFATC3 facilitated the progression of oral squamous cell carcinoma via the miR-520h/LDHA axis
The aim of this study was to verify the effects of circular RNA nuclear factor of activated T-cells, cytoplasmic 3 (circNFATC3), in oral squamous cell carcinoma (OSCC) development. The levels of circNFATC3, microRNA-520h (miR-520h), and lactate dehydrogenase A (LDHA) were measured by qRT-PCR and wes...
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| Format: | Article |
| Language: | English |
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De Gruyter
2023-06-01
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| Series: | Open Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/med-2023-0630 |
| _version_ | 1797788995881533440 |
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| author | Xie Hongguo Lu Xiaopeng |
| author_facet | Xie Hongguo Lu Xiaopeng |
| author_sort | Xie Hongguo |
| collection | DOAJ |
| description | The aim of this study was to verify the effects of circular RNA nuclear factor of activated T-cells, cytoplasmic 3 (circNFATC3), in oral squamous cell carcinoma (OSCC) development. The levels of circNFATC3, microRNA-520h (miR-520h), and lactate dehydrogenase A (LDHA) were measured by qRT-PCR and western blot analysis. The cellular functions were assessed by using commercial kits, MTT assay, EdU assay, flow cytometry analysis, and transwell assay. The interactions between miR-520h and circNFATC3 or LDHA were confirmed by dual-luciferase reporter assay. Finally, the mice test was enforced to evaluate the character of circNFATC3. We observed that the contents of circNFATC3 and LDHA were upregulated and miR-520h levels were downregulated in OSCC tissues compared with those in paracancerous tissues. For functional analysis, circNFATC3 knockdown repressed the cell glycolysis metabolism, cell proliferation, migration, and invasion, although it improved cell apoptosis in OSCC cells. LDHA could regulate the development of OSCC. circNFATC3 acted as a miR-520h sponge to modulate LDHA expression. In addition, the absence of circNFATC3 subdued tumor growth in vivo. In conclusion, circNFATC3 promoted the advancement of OSCC by adjusting the miR-520h/LDHA axis. |
| first_indexed | 2024-03-13T01:44:29Z |
| format | Article |
| id | doaj.art-35faaff4f81545ec97c97fcd739b2342 |
| institution | Directory Open Access Journal |
| issn | 2391-5463 |
| language | English |
| last_indexed | 2024-03-13T01:44:29Z |
| publishDate | 2023-06-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Medicine |
| spelling | doaj.art-35faaff4f81545ec97c97fcd739b23422023-07-03T10:19:45ZengDe GruyterOpen Medicine2391-54632023-06-0118173010.1515/med-2023-0630circNFATC3 facilitated the progression of oral squamous cell carcinoma via the miR-520h/LDHA axisXie Hongguo0Lu Xiaopeng1Department of Stomatology, Jingmen No. 1 People’s Hospital, Jingmen, 448000, Hubei, ChinaDepartment of Stomatology, Jingmen No. 1 People’s Hospital, No. 168, Xiangshan Avenue, Duodao District,, Jingmen, 448000, Hubei, ChinaThe aim of this study was to verify the effects of circular RNA nuclear factor of activated T-cells, cytoplasmic 3 (circNFATC3), in oral squamous cell carcinoma (OSCC) development. The levels of circNFATC3, microRNA-520h (miR-520h), and lactate dehydrogenase A (LDHA) were measured by qRT-PCR and western blot analysis. The cellular functions were assessed by using commercial kits, MTT assay, EdU assay, flow cytometry analysis, and transwell assay. The interactions between miR-520h and circNFATC3 or LDHA were confirmed by dual-luciferase reporter assay. Finally, the mice test was enforced to evaluate the character of circNFATC3. We observed that the contents of circNFATC3 and LDHA were upregulated and miR-520h levels were downregulated in OSCC tissues compared with those in paracancerous tissues. For functional analysis, circNFATC3 knockdown repressed the cell glycolysis metabolism, cell proliferation, migration, and invasion, although it improved cell apoptosis in OSCC cells. LDHA could regulate the development of OSCC. circNFATC3 acted as a miR-520h sponge to modulate LDHA expression. In addition, the absence of circNFATC3 subdued tumor growth in vivo. In conclusion, circNFATC3 promoted the advancement of OSCC by adjusting the miR-520h/LDHA axis.https://doi.org/10.1515/med-2023-0630oral squamous cell carcinomacircnfatc3mir-520hldhaglycolysis |
| spellingShingle | Xie Hongguo Lu Xiaopeng circNFATC3 facilitated the progression of oral squamous cell carcinoma via the miR-520h/LDHA axis Open Medicine oral squamous cell carcinoma circnfatc3 mir-520h ldha glycolysis |
| title | circNFATC3 facilitated the progression of oral squamous cell carcinoma via the miR-520h/LDHA axis |
| title_full | circNFATC3 facilitated the progression of oral squamous cell carcinoma via the miR-520h/LDHA axis |
| title_fullStr | circNFATC3 facilitated the progression of oral squamous cell carcinoma via the miR-520h/LDHA axis |
| title_full_unstemmed | circNFATC3 facilitated the progression of oral squamous cell carcinoma via the miR-520h/LDHA axis |
| title_short | circNFATC3 facilitated the progression of oral squamous cell carcinoma via the miR-520h/LDHA axis |
| title_sort | circnfatc3 facilitated the progression of oral squamous cell carcinoma via the mir 520h ldha axis |
| topic | oral squamous cell carcinoma circnfatc3 mir-520h ldha glycolysis |
| url | https://doi.org/10.1515/med-2023-0630 |
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