Egg Yolk Fat Deposition Is Regulated by Diacylglycerol and Ceramide Enriched by Adipocytokine Signaling Pathway in Laying Hens
The mechanism which regulates differential fat deposition in egg yolk from the indigenous breeds and commercial laying hens is still unclear. In this research, Chinese indigenous Huainan Partridge chickens and Nongda III commercial laying hens were used for egg collection and liver sampling. The wei...
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MDPI AG
2023-02-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/4/607 |
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| author | Qianyun Ji Penghui Chang Yuhao Dou Yutong Zhao Xingyong Chen |
| author_facet | Qianyun Ji Penghui Chang Yuhao Dou Yutong Zhao Xingyong Chen |
| author_sort | Qianyun Ji |
| collection | DOAJ |
| description | The mechanism which regulates differential fat deposition in egg yolk from the indigenous breeds and commercial laying hens is still unclear. In this research, Chinese indigenous Huainan Partridge chickens and Nongda III commercial laying hens were used for egg collection and liver sampling. The weight of eggs and yolk were recorded. Yolk fatty acids were determined by gas chromatography-mass spectrometry. Lipid metabolites in the liver were detected by liquid chromatography-mass spectrometry. Yolk weight, yolk ratio and yolk fat ratio exhibited higher in the Huainan Partridge chicken than that of the Nongda III. Compared to the Nongda III, the content of total saturated fatty acid was lower, while the unsaturated fatty acid was higher in the yolk of the Huainan Partridge chicken. Metabolites of phosphatidylinositol and phosphatidylserine from glycerolphospholipids, and metabolites of diacylglycerol from glycerolipids showed higher enrichment in the Huainan Partridge chicken than that of the Nongda III, which promoted the activation of the adipocytokine signaling pathway. However, metabolites of phosphatidic acid, phosphatidylcholine, phosphatidylethanolamine and lysophosphatidylcholine from glycerol phospholipids, and metabolites of triacylglycerol from glycerolipids showed lower enrichment in the Huainan Partridge chicken than that of the Nongda III. The high level of yolk fat deposition in the Huainan Partridge chicken is regulated by the activation of the adipocytokine signaling pathway which can promote the accumulation of diacylglycerol and ceramide in the liver. |
| first_indexed | 2024-03-11T09:15:48Z |
| format | Article |
| id | doaj.art-35fc2be24d524104a82cf59df3c7f003 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T09:15:48Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-35fc2be24d524104a82cf59df3c7f0032023-11-16T18:38:59ZengMDPI AGAnimals2076-26152023-02-0113460710.3390/ani13040607Egg Yolk Fat Deposition Is Regulated by Diacylglycerol and Ceramide Enriched by Adipocytokine Signaling Pathway in Laying HensQianyun Ji0Penghui Chang1Yuhao Dou2Yutong Zhao3Xingyong Chen4Collage of Animal Science and Technology, Anhui Agricultural University, No. 130 Changjiang West Road, Hefei 230036, ChinaCollage of Animal Science and Technology, Anhui Agricultural University, No. 130 Changjiang West Road, Hefei 230036, ChinaCollage of Animal Science and Technology, Anhui Agricultural University, No. 130 Changjiang West Road, Hefei 230036, ChinaCollage of Animal Science and Technology, Anhui Agricultural University, No. 130 Changjiang West Road, Hefei 230036, ChinaCollage of Animal Science and Technology, Anhui Agricultural University, No. 130 Changjiang West Road, Hefei 230036, ChinaThe mechanism which regulates differential fat deposition in egg yolk from the indigenous breeds and commercial laying hens is still unclear. In this research, Chinese indigenous Huainan Partridge chickens and Nongda III commercial laying hens were used for egg collection and liver sampling. The weight of eggs and yolk were recorded. Yolk fatty acids were determined by gas chromatography-mass spectrometry. Lipid metabolites in the liver were detected by liquid chromatography-mass spectrometry. Yolk weight, yolk ratio and yolk fat ratio exhibited higher in the Huainan Partridge chicken than that of the Nongda III. Compared to the Nongda III, the content of total saturated fatty acid was lower, while the unsaturated fatty acid was higher in the yolk of the Huainan Partridge chicken. Metabolites of phosphatidylinositol and phosphatidylserine from glycerolphospholipids, and metabolites of diacylglycerol from glycerolipids showed higher enrichment in the Huainan Partridge chicken than that of the Nongda III, which promoted the activation of the adipocytokine signaling pathway. However, metabolites of phosphatidic acid, phosphatidylcholine, phosphatidylethanolamine and lysophosphatidylcholine from glycerol phospholipids, and metabolites of triacylglycerol from glycerolipids showed lower enrichment in the Huainan Partridge chicken than that of the Nongda III. The high level of yolk fat deposition in the Huainan Partridge chicken is regulated by the activation of the adipocytokine signaling pathway which can promote the accumulation of diacylglycerol and ceramide in the liver.https://www.mdpi.com/2076-2615/13/4/607indigenous chickencommercial laying henyolk fat contentyolk fatty acidsliver metabolites |
| spellingShingle | Qianyun Ji Penghui Chang Yuhao Dou Yutong Zhao Xingyong Chen Egg Yolk Fat Deposition Is Regulated by Diacylglycerol and Ceramide Enriched by Adipocytokine Signaling Pathway in Laying Hens Animals indigenous chicken commercial laying hen yolk fat content yolk fatty acids liver metabolites |
| title | Egg Yolk Fat Deposition Is Regulated by Diacylglycerol and Ceramide Enriched by Adipocytokine Signaling Pathway in Laying Hens |
| title_full | Egg Yolk Fat Deposition Is Regulated by Diacylglycerol and Ceramide Enriched by Adipocytokine Signaling Pathway in Laying Hens |
| title_fullStr | Egg Yolk Fat Deposition Is Regulated by Diacylglycerol and Ceramide Enriched by Adipocytokine Signaling Pathway in Laying Hens |
| title_full_unstemmed | Egg Yolk Fat Deposition Is Regulated by Diacylglycerol and Ceramide Enriched by Adipocytokine Signaling Pathway in Laying Hens |
| title_short | Egg Yolk Fat Deposition Is Regulated by Diacylglycerol and Ceramide Enriched by Adipocytokine Signaling Pathway in Laying Hens |
| title_sort | egg yolk fat deposition is regulated by diacylglycerol and ceramide enriched by adipocytokine signaling pathway in laying hens |
| topic | indigenous chicken commercial laying hen yolk fat content yolk fatty acids liver metabolites |
| url | https://www.mdpi.com/2076-2615/13/4/607 |
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