Chromosomal and Genetic Analysis of a Human Lung Adenocarcinoma Cell Line OM
Background: Lung cancer has become the leading cause of death in many regions. Carcinogenesis is caused by the stepwise accumulation of genetic and chromosomal changes. The aim of this study was to investigate the chromosome and gene alterations in the human lung adenocarcinoma cell line OM. Methods...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer
2016-01-01
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| Series: | Chinese Medical Journal |
| Subjects: | |
| Online Access: | http://www.cmj.org/article.asp?issn=0366-6999;year=2016;volume=129;issue=4;spage=405;epage=409;aulast=Li |
| _version_ | 1828519110659538944 |
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| author | Yong-Wu Li Lin Bai Lyu-Xia Dai Xu He Xian-Ping Zhou |
| author_facet | Yong-Wu Li Lin Bai Lyu-Xia Dai Xu He Xian-Ping Zhou |
| author_sort | Yong-Wu Li |
| collection | DOAJ |
| description | Background: Lung cancer has become the leading cause of death in many regions. Carcinogenesis is caused by the stepwise accumulation of genetic and chromosomal changes. The aim of this study was to investigate the chromosome and gene alterations in the human lung adenocarcinoma cell line OM.
Methods: We used Giemsa banding and multiplex fluorescence in situ hybridization focusing on the human lung adenocarcinoma cell line OM to analyze its chromosome alterations. In addition, the gains and losses in the specific chromosome regions were identified by comparative genomic hybridization (CGH) and the amplifications of cancer-related genes were also detected by polymerase chain reaction (PCR).
Results: We identified a large number of chromosomal numerical alterations on all chromosomes except chromosome X and 19. Chromosome 10 is the most frequently involved in translocations with six different interchromosomal translocations. CGH revealed the gains on chromosome regions of 3q25.3-28, 5p13, 12q22-23.24, and the losses on 3p25-26, 6p25, 6q26-27, 7q34-36, 8p22-23, 9p21-24, 10q25-26.3, 12p13.31-13.33 and 17p13.1-13.3. And PCR showed the amplification of genes: Membrane metalloendopeptidase (MME), sucrase-isomaltase (SI), butyrylcholinesterase (BCHE), and kininogen (KNG).
Conclusions: The lung adenocarcinoma cell line OM exhibited multiple complex karyotypes, and chromosome 10 was frequently involved in chromosomal translocation, which may play key roles in tumorigenesis. We speculated that the oncogenes may be located at 3q25.3-28, 5p13, 12q22-23.24, while tumor suppressor genes may exist in 3p25-26, 6p25, 6q26-27, 7q34-36, 8p22-23, 9p21-24, 10q25-26.3, 12p13.31-13.33, and 17p13.1-13.3. Moreover, at least four genes (MME, SI, BCHE, and KNG) may be involved in the human lung adenocarcinoma cell line OM. |
| first_indexed | 2024-12-11T19:06:26Z |
| format | Article |
| id | doaj.art-3601e193bede43a6bbf8e02f0726409f |
| institution | Directory Open Access Journal |
| issn | 0366-6999 |
| language | English |
| last_indexed | 2024-12-11T19:06:26Z |
| publishDate | 2016-01-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Chinese Medical Journal |
| spelling | doaj.art-3601e193bede43a6bbf8e02f0726409f2022-12-22T00:53:52ZengWolters KluwerChinese Medical Journal0366-69992016-01-01129440540910.4103/0366-6999.176066Chromosomal and Genetic Analysis of a Human Lung Adenocarcinoma Cell Line OMYong-Wu LiLin BaiLyu-Xia DaiXu HeXian-Ping ZhouBackground: Lung cancer has become the leading cause of death in many regions. Carcinogenesis is caused by the stepwise accumulation of genetic and chromosomal changes. The aim of this study was to investigate the chromosome and gene alterations in the human lung adenocarcinoma cell line OM. Methods: We used Giemsa banding and multiplex fluorescence in situ hybridization focusing on the human lung adenocarcinoma cell line OM to analyze its chromosome alterations. In addition, the gains and losses in the specific chromosome regions were identified by comparative genomic hybridization (CGH) and the amplifications of cancer-related genes were also detected by polymerase chain reaction (PCR). Results: We identified a large number of chromosomal numerical alterations on all chromosomes except chromosome X and 19. Chromosome 10 is the most frequently involved in translocations with six different interchromosomal translocations. CGH revealed the gains on chromosome regions of 3q25.3-28, 5p13, 12q22-23.24, and the losses on 3p25-26, 6p25, 6q26-27, 7q34-36, 8p22-23, 9p21-24, 10q25-26.3, 12p13.31-13.33 and 17p13.1-13.3. And PCR showed the amplification of genes: Membrane metalloendopeptidase (MME), sucrase-isomaltase (SI), butyrylcholinesterase (BCHE), and kininogen (KNG). Conclusions: The lung adenocarcinoma cell line OM exhibited multiple complex karyotypes, and chromosome 10 was frequently involved in chromosomal translocation, which may play key roles in tumorigenesis. We speculated that the oncogenes may be located at 3q25.3-28, 5p13, 12q22-23.24, while tumor suppressor genes may exist in 3p25-26, 6p25, 6q26-27, 7q34-36, 8p22-23, 9p21-24, 10q25-26.3, 12p13.31-13.33, and 17p13.1-13.3. Moreover, at least four genes (MME, SI, BCHE, and KNG) may be involved in the human lung adenocarcinoma cell line OM.http://www.cmj.org/article.asp?issn=0366-6999;year=2016;volume=129;issue=4;spage=405;epage=409;aulast=LiCell Line; Chromosomes; Genes; Lung Cancer |
| spellingShingle | Yong-Wu Li Lin Bai Lyu-Xia Dai Xu He Xian-Ping Zhou Chromosomal and Genetic Analysis of a Human Lung Adenocarcinoma Cell Line OM Chinese Medical Journal Cell Line; Chromosomes; Genes; Lung Cancer |
| title | Chromosomal and Genetic Analysis of a Human Lung Adenocarcinoma Cell Line OM |
| title_full | Chromosomal and Genetic Analysis of a Human Lung Adenocarcinoma Cell Line OM |
| title_fullStr | Chromosomal and Genetic Analysis of a Human Lung Adenocarcinoma Cell Line OM |
| title_full_unstemmed | Chromosomal and Genetic Analysis of a Human Lung Adenocarcinoma Cell Line OM |
| title_short | Chromosomal and Genetic Analysis of a Human Lung Adenocarcinoma Cell Line OM |
| title_sort | chromosomal and genetic analysis of a human lung adenocarcinoma cell line om |
| topic | Cell Line; Chromosomes; Genes; Lung Cancer |
| url | http://www.cmj.org/article.asp?issn=0366-6999;year=2016;volume=129;issue=4;spage=405;epage=409;aulast=Li |
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