Characterization and organelle genome sequencing of Pyropia species from Myanmar
Abstract Pyropia is a genus comprising red algae of the Bangiaceae family that is commonly found in intertidal zones worldwide. However, understanding of Pyropia species that are prone to tropical regions remains limited despite recent breakthroughs in genomic research. Within the realm of Pyropia s...
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| Format: | Article |
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Nature Portfolio
2023-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-42262-3 |
| _version_ | 1797453263495233536 |
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| author | Myat Htoo San Yoshio Kawamura Kei Kimura Eranga Pawani Witharana Takeshi Shimogiri San San Aye Thu Thu Min Cherry Aung Moe Moe Khaing Yukio Nagano |
| author_facet | Myat Htoo San Yoshio Kawamura Kei Kimura Eranga Pawani Witharana Takeshi Shimogiri San San Aye Thu Thu Min Cherry Aung Moe Moe Khaing Yukio Nagano |
| author_sort | Myat Htoo San |
| collection | DOAJ |
| description | Abstract Pyropia is a genus comprising red algae of the Bangiaceae family that is commonly found in intertidal zones worldwide. However, understanding of Pyropia species that are prone to tropical regions remains limited despite recent breakthroughs in genomic research. Within the realm of Pyropia species thriving in tropical regions, P. vietnamensis stands out as a widely recognized species. In this study, we aimed to investigate Pyropia species in the southwest coast of Myanmar using physiological and molecular approaches, culture-based analyses, chloroplast rbcL and nuclear SSU gene sequencing, and whole chloroplast and mitochondrial genome sequencing. Physiological analysis showed that the Myanmar samples were more heat-tolerant than their Japanese counterparts, including those of subtropical origin. Additionally, molecular characterization revealed that the Myanmar samples were closely related to P. vietnamensis from India. This study is the first to sequence the chloroplast and mitochondrial genomes of Pyropia species from tropical regions. A unique deletion event was observed within a ribosomal RNA gene cluster in the chloroplast genome of the studied Pyropia species, which is a deviation from the usual characteristics of most Pyropia species. This study improves current understanding of the physiological and molecular characteristics of this comparatively understudied Pyropia species that grows in tropical regions. |
| first_indexed | 2024-03-09T15:20:18Z |
| format | Article |
| id | doaj.art-360299b9810b469d867f0d859ac660ac |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:20:18Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj.art-360299b9810b469d867f0d859ac660ac2023-11-26T12:49:16ZengNature PortfolioScientific Reports2045-23222023-09-0113111610.1038/s41598-023-42262-3Characterization and organelle genome sequencing of Pyropia species from MyanmarMyat Htoo San0Yoshio Kawamura1Kei Kimura2Eranga Pawani Witharana3Takeshi Shimogiri4San San Aye5Thu Thu Min6Cherry Aung7Moe Moe Khaing8Yukio Nagano9The United Graduate School of Agricultural Sciences, Kagoshima UniversityFaculty of Agriculture, Saga UniversityThe United Graduate School of Agricultural Sciences, Kagoshima UniversityGraduate School of Advanced Health Science, Saga UniversityThe United Graduate School of Agricultural Sciences, Kagoshima UniversityMawlamyine UniversityMarine Science Department, Pathein UniversityMarine Science Department, Myeik UniversityBotany Department, Dawei UniversityThe United Graduate School of Agricultural Sciences, Kagoshima UniversityAbstract Pyropia is a genus comprising red algae of the Bangiaceae family that is commonly found in intertidal zones worldwide. However, understanding of Pyropia species that are prone to tropical regions remains limited despite recent breakthroughs in genomic research. Within the realm of Pyropia species thriving in tropical regions, P. vietnamensis stands out as a widely recognized species. In this study, we aimed to investigate Pyropia species in the southwest coast of Myanmar using physiological and molecular approaches, culture-based analyses, chloroplast rbcL and nuclear SSU gene sequencing, and whole chloroplast and mitochondrial genome sequencing. Physiological analysis showed that the Myanmar samples were more heat-tolerant than their Japanese counterparts, including those of subtropical origin. Additionally, molecular characterization revealed that the Myanmar samples were closely related to P. vietnamensis from India. This study is the first to sequence the chloroplast and mitochondrial genomes of Pyropia species from tropical regions. A unique deletion event was observed within a ribosomal RNA gene cluster in the chloroplast genome of the studied Pyropia species, which is a deviation from the usual characteristics of most Pyropia species. This study improves current understanding of the physiological and molecular characteristics of this comparatively understudied Pyropia species that grows in tropical regions.https://doi.org/10.1038/s41598-023-42262-3 |
| spellingShingle | Myat Htoo San Yoshio Kawamura Kei Kimura Eranga Pawani Witharana Takeshi Shimogiri San San Aye Thu Thu Min Cherry Aung Moe Moe Khaing Yukio Nagano Characterization and organelle genome sequencing of Pyropia species from Myanmar Scientific Reports |
| title | Characterization and organelle genome sequencing of Pyropia species from Myanmar |
| title_full | Characterization and organelle genome sequencing of Pyropia species from Myanmar |
| title_fullStr | Characterization and organelle genome sequencing of Pyropia species from Myanmar |
| title_full_unstemmed | Characterization and organelle genome sequencing of Pyropia species from Myanmar |
| title_short | Characterization and organelle genome sequencing of Pyropia species from Myanmar |
| title_sort | characterization and organelle genome sequencing of pyropia species from myanmar |
| url | https://doi.org/10.1038/s41598-023-42262-3 |
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