Prevalence and molecular characterization of some circulating strains of the peste-des-petits-ruminants virus in Saudi Arabia between 2014–2016
Background The peste-des-petits-ruminants virus (PPRV) is a highly devastating virus of small ruminants in many parts of the world, including the Kingdome of Saudi Arabia. Therefore, our objectives were (1) to conduct a molecular prevalence study of PPRV in sheep and goat across the KSA and (2) to i...
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PeerJ Inc.
2020-05-01
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| Online Access: | https://peerj.com/articles/9035.pdf |
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| author | Maged Gomaa Hemida Hussain Mohammed Alghadeer Mohammed Alhammadi Sayed Ali |
| author_facet | Maged Gomaa Hemida Hussain Mohammed Alghadeer Mohammed Alhammadi Sayed Ali |
| author_sort | Maged Gomaa Hemida |
| collection | DOAJ |
| description | Background The peste-des-petits-ruminants virus (PPRV) is a highly devastating virus of small ruminants in many parts of the world, including the Kingdome of Saudi Arabia. Therefore, our objectives were (1) to conduct a molecular prevalence study of PPRV in sheep and goat across the KSA and (2) to isolate and identify currently circulating PPRV lineages. Swabs and tissue specimens were collected from 97 herds suspected to be infected with PPRV across the Kingdome of Saudi Arabia (KSA). Testing for the presence of the virus was done by the real-time PCR. Confirmation of the identity of the reactions was done by the gel-based-PCR then by sequencing of the partial PPRV genome. Results Our results revealed that 24.1% of the tested specimens were PPRV-positive. Isolation of PPRV was successful from samples using the Vero cell line. Sequence analysis of some partial PPRV genes (N, F, M, L, P, and H) revealed that these strains were belonging to lineage IV of the PPRV. Conclusions This is the first study to conduct both the nationwide prevalence, isolation, and molecular characterizations of the PPRV in the KSA. Continuous surveillance and monitoring of the circulating strains of PPRV among sheep and goats will contribute substantially to the global eradication campaign of such a virus. |
| first_indexed | 2024-03-09T06:25:40Z |
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| id | doaj.art-360aac560e064b5a9888eaf9772848b5 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:25:40Z |
| publishDate | 2020-05-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-360aac560e064b5a9888eaf9772848b52023-12-03T11:19:44ZengPeerJ Inc.PeerJ2167-83592020-05-018e903510.7717/peerj.9035Prevalence and molecular characterization of some circulating strains of the peste-des-petits-ruminants virus in Saudi Arabia between 2014–2016Maged Gomaa Hemida0Hussain Mohammed Alghadeer1Mohammed Alhammadi2Sayed Ali3Department of Microbiology, College of Veterinary Medicine, King Faisal University, Al-Hufuf, Al-Hasa, Saudi ArabiaDepartment of Virology, Central Veterinary Diagnostic Laboratory, Minstry of Water, Enviroment and Agriculture, Riyadh, Riyadh, Saudi ArabiaDepartment of Microbiology, College of Veterinary Medicine, King Faisal University, Al-Hufuf, Al-Hasa, Saudi ArabiaDepartment of Family and Community Medicine, College of Medicine, King Faisal University, Al-Hufuf, Al-Hasa, Saudi ArabiaBackground The peste-des-petits-ruminants virus (PPRV) is a highly devastating virus of small ruminants in many parts of the world, including the Kingdome of Saudi Arabia. Therefore, our objectives were (1) to conduct a molecular prevalence study of PPRV in sheep and goat across the KSA and (2) to isolate and identify currently circulating PPRV lineages. Swabs and tissue specimens were collected from 97 herds suspected to be infected with PPRV across the Kingdome of Saudi Arabia (KSA). Testing for the presence of the virus was done by the real-time PCR. Confirmation of the identity of the reactions was done by the gel-based-PCR then by sequencing of the partial PPRV genome. Results Our results revealed that 24.1% of the tested specimens were PPRV-positive. Isolation of PPRV was successful from samples using the Vero cell line. Sequence analysis of some partial PPRV genes (N, F, M, L, P, and H) revealed that these strains were belonging to lineage IV of the PPRV. Conclusions This is the first study to conduct both the nationwide prevalence, isolation, and molecular characterizations of the PPRV in the KSA. Continuous surveillance and monitoring of the circulating strains of PPRV among sheep and goats will contribute substantially to the global eradication campaign of such a virus.https://peerj.com/articles/9035.pdfSRMVELISAPCRReal Time PCRSmall ruminantslineage IV |
| spellingShingle | Maged Gomaa Hemida Hussain Mohammed Alghadeer Mohammed Alhammadi Sayed Ali Prevalence and molecular characterization of some circulating strains of the peste-des-petits-ruminants virus in Saudi Arabia between 2014–2016 PeerJ SRMV ELISA PCR Real Time PCR Small ruminants lineage IV |
| title | Prevalence and molecular characterization of some circulating strains of the peste-des-petits-ruminants virus in Saudi Arabia between 2014–2016 |
| title_full | Prevalence and molecular characterization of some circulating strains of the peste-des-petits-ruminants virus in Saudi Arabia between 2014–2016 |
| title_fullStr | Prevalence and molecular characterization of some circulating strains of the peste-des-petits-ruminants virus in Saudi Arabia between 2014–2016 |
| title_full_unstemmed | Prevalence and molecular characterization of some circulating strains of the peste-des-petits-ruminants virus in Saudi Arabia between 2014–2016 |
| title_short | Prevalence and molecular characterization of some circulating strains of the peste-des-petits-ruminants virus in Saudi Arabia between 2014–2016 |
| title_sort | prevalence and molecular characterization of some circulating strains of the peste des petits ruminants virus in saudi arabia between 2014 2016 |
| topic | SRMV ELISA PCR Real Time PCR Small ruminants lineage IV |
| url | https://peerj.com/articles/9035.pdf |
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