Fine Mapping and Candidate Gene Identification for Wax Biosynthesis Locus, BoWax1 in Brassica oleracea L. var. capitata
Cuticular waxes play important roles in plant protection against various biotic and abiotic environmental stresses. The cuticular wax covering gives normal cabbage a glaucous appearance, but the appearance of waxless mutant is glossy green. Based on the present study, inheritance of the glossy green...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2018.00309/full |
| _version_ | 1818062507798953984 |
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| author | Dongming Liu Dongming Liu Dongming Liu Xin Dong Zezhou Liu Jun Tang Mu Zhuang Yangyong Zhang Honghao Lv Yumei Liu Zhansheng Li Zhiyuan Fang Limei Yang |
| author_facet | Dongming Liu Dongming Liu Dongming Liu Xin Dong Zezhou Liu Jun Tang Mu Zhuang Yangyong Zhang Honghao Lv Yumei Liu Zhansheng Li Zhiyuan Fang Limei Yang |
| author_sort | Dongming Liu |
| collection | DOAJ |
| description | Cuticular waxes play important roles in plant protection against various biotic and abiotic environmental stresses. The cuticular wax covering gives normal cabbage a glaucous appearance, but the appearance of waxless mutant is glossy green. Based on the present study, inheritance of the glossy green character of mutant HUAYOU2 follows a simple recessive pattern. Genetic analysis of an F2 population comprising 808 recessive individuals derived from HUAYOU2 (P1, maternal parent) and M36 (P2, paternal parent) revealed that a single recessive locus, BoWax1 (Brassica oleracea Wax 1), controls glossy green trait in B. oleracea. This locus was mapped to a region of 158.5 kb on chromosome C01. Based on nucleotide sequence analysis, Bol013612 was identified as the candidate gene for BoWax1. Sequencing results demonstrated that there is a deletion mutation of two nucleotides in the cDNA of Bol013612 of HUAYOU2, which may account for its glossy green trait. These results lay the foundation for functional analysis of BoWax1 and may accelerate research on wax metabolism in cabbage. |
| first_indexed | 2024-12-10T14:05:19Z |
| format | Article |
| id | doaj.art-360c612f187b445c89f83a41dcddfef2 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T14:05:19Z |
| publishDate | 2018-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-360c612f187b445c89f83a41dcddfef22022-12-22T01:45:41ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-04-01910.3389/fpls.2018.00309287563Fine Mapping and Candidate Gene Identification for Wax Biosynthesis Locus, BoWax1 in Brassica oleracea L. var. capitataDongming Liu0Dongming Liu1Dongming Liu2Xin Dong3Zezhou Liu4Jun Tang5Mu Zhuang6Yangyong Zhang7Honghao Lv8Yumei Liu9Zhansheng Li10Zhiyuan Fang11Limei Yang12Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaOpen Key Laboratory of Horticultural Plant Physiology and Genetic Improvement, High School of Henan Province, College of Horticulture, Henan Agricultural University, Zhengzhou, ChinaInstitute of Horticulture, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaCuticular waxes play important roles in plant protection against various biotic and abiotic environmental stresses. The cuticular wax covering gives normal cabbage a glaucous appearance, but the appearance of waxless mutant is glossy green. Based on the present study, inheritance of the glossy green character of mutant HUAYOU2 follows a simple recessive pattern. Genetic analysis of an F2 population comprising 808 recessive individuals derived from HUAYOU2 (P1, maternal parent) and M36 (P2, paternal parent) revealed that a single recessive locus, BoWax1 (Brassica oleracea Wax 1), controls glossy green trait in B. oleracea. This locus was mapped to a region of 158.5 kb on chromosome C01. Based on nucleotide sequence analysis, Bol013612 was identified as the candidate gene for BoWax1. Sequencing results demonstrated that there is a deletion mutation of two nucleotides in the cDNA of Bol013612 of HUAYOU2, which may account for its glossy green trait. These results lay the foundation for functional analysis of BoWax1 and may accelerate research on wax metabolism in cabbage.http://journal.frontiersin.org/article/10.3389/fpls.2018.00309/fullcabbageglossy green mutantinheritancegene mappingcandidate gene |
| spellingShingle | Dongming Liu Dongming Liu Dongming Liu Xin Dong Zezhou Liu Jun Tang Mu Zhuang Yangyong Zhang Honghao Lv Yumei Liu Zhansheng Li Zhiyuan Fang Limei Yang Fine Mapping and Candidate Gene Identification for Wax Biosynthesis Locus, BoWax1 in Brassica oleracea L. var. capitata Frontiers in Plant Science cabbage glossy green mutant inheritance gene mapping candidate gene |
| title | Fine Mapping and Candidate Gene Identification for Wax Biosynthesis Locus, BoWax1 in Brassica oleracea L. var. capitata |
| title_full | Fine Mapping and Candidate Gene Identification for Wax Biosynthesis Locus, BoWax1 in Brassica oleracea L. var. capitata |
| title_fullStr | Fine Mapping and Candidate Gene Identification for Wax Biosynthesis Locus, BoWax1 in Brassica oleracea L. var. capitata |
| title_full_unstemmed | Fine Mapping and Candidate Gene Identification for Wax Biosynthesis Locus, BoWax1 in Brassica oleracea L. var. capitata |
| title_short | Fine Mapping and Candidate Gene Identification for Wax Biosynthesis Locus, BoWax1 in Brassica oleracea L. var. capitata |
| title_sort | fine mapping and candidate gene identification for wax biosynthesis locus bowax1 in brassica oleracea l var capitata |
| topic | cabbage glossy green mutant inheritance gene mapping candidate gene |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2018.00309/full |
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