Dietary supplementation with spray-dried animal plasma improves vaccine protection in aged mice
BackgroundSenescence is characterized by an aggravated inflammatory state that reduces vaccine responsiveness. Dietary supplementation with spray-dried porcine plasma (SDP) exerts anti-inflammatory effects in different mucosal areas. We aimed to determine if the anti-inflammatory properties of SDP i...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2023.1050961/full |
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| author | Lluïsa Miró Cristina Rosell-Cardona Concepció Amat Javier Polo Miquel Moretó Anna Pérez-Bosque |
| author_facet | Lluïsa Miró Cristina Rosell-Cardona Concepció Amat Javier Polo Miquel Moretó Anna Pérez-Bosque |
| author_sort | Lluïsa Miró |
| collection | DOAJ |
| description | BackgroundSenescence is characterized by an aggravated inflammatory state that reduces vaccine responsiveness. Dietary supplementation with spray-dried porcine plasma (SDP) exerts anti-inflammatory effects in different mucosal areas. We aimed to determine if the anti-inflammatory properties of SDP improve the efficiency of immunization in senescent animals.MethodsExperiments were performed in 2-month-old and 6-month-old male SAMP8 mice fed control or SDP (8%) feeds for 4 months. The mice received nasal doses of 2.5 μg of Staphylococcus aureus enterotoxin B (SEB) or vehicle every 15 days (i.e., 3 times). Fifteen days after the last dose, a lethal shock was induced by intraperitoneal administration of SEB and LPS.ResultsImmunization increased anti-SEB IgA in intestinal and bronchoalveolar fluid (p < 0.05). After the lethal shock, all immunized aged mice that were supplemented with SDP survived, in contrast to only 66% of those fed the control feed (p < 0.05). Moreover, after the lethal challenge, aged mice showed higher expression levels of pro-inflammatory cytokines (Il-6, Tnf-α, Ifn-γ, and Il-1β) in jejunal and (Tnf-α, and Il-1β) in lung tissues (p < 0.05), which were reduced by SDP supplementation (p < 0.05). Furthermore, in senescent mice, SDP supplementation augmented Il-4 and Il-10 expression in both tissues (p < 0.05).ConclusionSDP reduces the mucosal inflammation associated with aging, improving vaccine protection in senescent mice. |
| first_indexed | 2024-04-09T21:52:56Z |
| format | Article |
| id | doaj.art-360d1eb9fdf64360ad2cfedc08bdd081 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-09T21:52:56Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-360d1eb9fdf64360ad2cfedc08bdd0812023-03-24T11:34:18ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2023-03-011010.3389/fnut.2023.10509611050961Dietary supplementation with spray-dried animal plasma improves vaccine protection in aged miceLluïsa Miró0Cristina Rosell-Cardona1Concepció Amat2Javier Polo3Miquel Moretó4Anna Pérez-Bosque5Departament de Bioquímica i Fisiologia, Facultat de Farmàcia i Ciències de l’Alimentació and Institut de Recerca en Nutrició i Seguretat Alimentària (INSA-UB), Universitat de Barcelona (UB), Barcelona, SpainDepartament de Bioquímica i Fisiologia, Facultat de Farmàcia i Ciències de l’Alimentació and Institut de Recerca en Nutrició i Seguretat Alimentària (INSA-UB), Universitat de Barcelona (UB), Barcelona, SpainDepartament de Bioquímica i Fisiologia, Facultat de Farmàcia i Ciències de l’Alimentació and Institut de Recerca en Nutrició i Seguretat Alimentària (INSA-UB), Universitat de Barcelona (UB), Barcelona, SpainAPC-Europe S.L.U., Granollers, SpainDepartament de Bioquímica i Fisiologia, Facultat de Farmàcia i Ciències de l’Alimentació and Institut de Recerca en Nutrició i Seguretat Alimentària (INSA-UB), Universitat de Barcelona (UB), Barcelona, SpainDepartament de Bioquímica i Fisiologia, Facultat de Farmàcia i Ciències de l’Alimentació and Institut de Recerca en Nutrició i Seguretat Alimentària (INSA-UB), Universitat de Barcelona (UB), Barcelona, SpainBackgroundSenescence is characterized by an aggravated inflammatory state that reduces vaccine responsiveness. Dietary supplementation with spray-dried porcine plasma (SDP) exerts anti-inflammatory effects in different mucosal areas. We aimed to determine if the anti-inflammatory properties of SDP improve the efficiency of immunization in senescent animals.MethodsExperiments were performed in 2-month-old and 6-month-old male SAMP8 mice fed control or SDP (8%) feeds for 4 months. The mice received nasal doses of 2.5 μg of Staphylococcus aureus enterotoxin B (SEB) or vehicle every 15 days (i.e., 3 times). Fifteen days after the last dose, a lethal shock was induced by intraperitoneal administration of SEB and LPS.ResultsImmunization increased anti-SEB IgA in intestinal and bronchoalveolar fluid (p < 0.05). After the lethal shock, all immunized aged mice that were supplemented with SDP survived, in contrast to only 66% of those fed the control feed (p < 0.05). Moreover, after the lethal challenge, aged mice showed higher expression levels of pro-inflammatory cytokines (Il-6, Tnf-α, Ifn-γ, and Il-1β) in jejunal and (Tnf-α, and Il-1β) in lung tissues (p < 0.05), which were reduced by SDP supplementation (p < 0.05). Furthermore, in senescent mice, SDP supplementation augmented Il-4 and Il-10 expression in both tissues (p < 0.05).ConclusionSDP reduces the mucosal inflammation associated with aging, improving vaccine protection in senescent mice.https://www.frontiersin.org/articles/10.3389/fnut.2023.1050961/fullsenescenceimmune protectionimmunizationdietary supplementationspray-dried porcine plasma |
| spellingShingle | Lluïsa Miró Cristina Rosell-Cardona Concepció Amat Javier Polo Miquel Moretó Anna Pérez-Bosque Dietary supplementation with spray-dried animal plasma improves vaccine protection in aged mice Frontiers in Nutrition senescence immune protection immunization dietary supplementation spray-dried porcine plasma |
| title | Dietary supplementation with spray-dried animal plasma improves vaccine protection in aged mice |
| title_full | Dietary supplementation with spray-dried animal plasma improves vaccine protection in aged mice |
| title_fullStr | Dietary supplementation with spray-dried animal plasma improves vaccine protection in aged mice |
| title_full_unstemmed | Dietary supplementation with spray-dried animal plasma improves vaccine protection in aged mice |
| title_short | Dietary supplementation with spray-dried animal plasma improves vaccine protection in aged mice |
| title_sort | dietary supplementation with spray dried animal plasma improves vaccine protection in aged mice |
| topic | senescence immune protection immunization dietary supplementation spray-dried porcine plasma |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2023.1050961/full |
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