Characterization of Leukocyte- and Platelet-Rich Plasma Derived from Female Collage Athletes: A Cross-Sectional Cohort Study Focusing on Growth Factor, Inflammatory Cytokines, and Anti-Inflammatory Cytokine Levels
Platelet-rich plasma (PRP) has been increasingly used in sports medicine owing to its various advantages. The purpose of our project was to standardize the parameters before performing large-scale clinical trials in the near future to precisely evaluate individual PRP quality. To examine the effects...
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/17/13592 |
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| author | Tomoharu Mochizuki Takashi Ushiki Katsuya Suzuki Misato Sato Hajime Ishiguro Tatsuya Suwabe Mutsuaki Edama Go Omori Noriaki Yamamoto Tomoyuki Kawase |
| author_facet | Tomoharu Mochizuki Takashi Ushiki Katsuya Suzuki Misato Sato Hajime Ishiguro Tatsuya Suwabe Mutsuaki Edama Go Omori Noriaki Yamamoto Tomoyuki Kawase |
| author_sort | Tomoharu Mochizuki |
| collection | DOAJ |
| description | Platelet-rich plasma (PRP) has been increasingly used in sports medicine owing to its various advantages. The purpose of our project was to standardize the parameters before performing large-scale clinical trials in the near future to precisely evaluate individual PRP quality. To examine the effects of regular exercise on PRP quality, this study focused on young female athletes, who have been relatively less studied. Blood samples were obtained from female college athletes (n = 35) and ordinary healthy adults (n = 30), which were considered as controls, and leukocyte-rich PRP (L-PRP) was prepared manually. Body composition indices were determined using a bathroom weight scale equipped with an impedance meter. Growth factors and cytokines were quantified using ELISA kits. Platelet-derived growth factor-BB (PDGF-BB) and Transforming-growth factors β1 (TGFβ1) levels (per platelet) in L-PRP were significantly lower in female athletes than in controls. In contrast, Interleukin-1β and Interleukin 1 receptor antagonist (IL-1RA) levels (per platelet and L-PRP) in L-PRP were significantly higher in athletes, and this difference was more prominent in IL-1RA. These findings suggest that L-PRP from athletes may facilitate the inflammatory phase of the healing process by regulating the pro-inflammatory and anti-inflammatory balance. These chemical compositions can be adopted as “must-check” parameters to characterize individual PRP preparations prior to clinical trials. |
| first_indexed | 2024-03-10T23:20:16Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T23:20:16Z |
| publishDate | 2023-09-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-361210c4b61349f79a4368975d05fcd32023-11-19T08:19:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124171359210.3390/ijms241713592Characterization of Leukocyte- and Platelet-Rich Plasma Derived from Female Collage Athletes: A Cross-Sectional Cohort Study Focusing on Growth Factor, Inflammatory Cytokines, and Anti-Inflammatory Cytokine LevelsTomoharu Mochizuki0Takashi Ushiki1Katsuya Suzuki2Misato Sato3Hajime Ishiguro4Tatsuya Suwabe5Mutsuaki Edama6Go Omori7Noriaki Yamamoto8Tomoyuki Kawase9Department of Orthopaedic Surgery, Graduate School of Medical and Dental Sciences, Niigata University, Niigata 951-8510, JapanDivision of Hematology and Oncology, Graduate School of Health Sciences, Niigata University, Niigata 951-9518, JapanDepartment of Transfusion Medicine, Cell Therapy and Regenerative Medicine, Niigata University Medical and Dental Hospital, Niigata 951-8520, JapanDepartment of Transfusion Medicine, Cell Therapy and Regenerative Medicine, Niigata University Medical and Dental Hospital, Niigata 951-8520, JapanDepartment of Hematology, Endocrinology and Metabolism, Faculty of Medicine, Niigata University, Niigata 951-8510, JapanDepartment of Hematology, Endocrinology and Metabolism, Faculty of Medicine, Niigata University, Niigata 951-8510, JapanDepartment of Health and Sports, Faculty of Health Sciences, Niigata University of Health and Welfare, Niigata 950-3102, JapanDepartment of Health and Sports, Faculty of Health Sciences, Niigata University of Health and Welfare, Niigata 950-3102, JapanDepartment of Orthopaedic Surgery, Niigata Rehabilitation Hospital, Niigata 950-3304, JapanDivision of Oral Bioengineering, Graduate School of Medical and Dental Sciences, Niigata University, Niigata 951-8514, JapanPlatelet-rich plasma (PRP) has been increasingly used in sports medicine owing to its various advantages. The purpose of our project was to standardize the parameters before performing large-scale clinical trials in the near future to precisely evaluate individual PRP quality. To examine the effects of regular exercise on PRP quality, this study focused on young female athletes, who have been relatively less studied. Blood samples were obtained from female college athletes (n = 35) and ordinary healthy adults (n = 30), which were considered as controls, and leukocyte-rich PRP (L-PRP) was prepared manually. Body composition indices were determined using a bathroom weight scale equipped with an impedance meter. Growth factors and cytokines were quantified using ELISA kits. Platelet-derived growth factor-BB (PDGF-BB) and Transforming-growth factors β1 (TGFβ1) levels (per platelet) in L-PRP were significantly lower in female athletes than in controls. In contrast, Interleukin-1β and Interleukin 1 receptor antagonist (IL-1RA) levels (per platelet and L-PRP) in L-PRP were significantly higher in athletes, and this difference was more prominent in IL-1RA. These findings suggest that L-PRP from athletes may facilitate the inflammatory phase of the healing process by regulating the pro-inflammatory and anti-inflammatory balance. These chemical compositions can be adopted as “must-check” parameters to characterize individual PRP preparations prior to clinical trials.https://www.mdpi.com/1422-0067/24/17/13592leukocyte- and platelet-rich plasmafemale college athletesPDGF-BBTGFβ1IL-1βIL-1RA |
| spellingShingle | Tomoharu Mochizuki Takashi Ushiki Katsuya Suzuki Misato Sato Hajime Ishiguro Tatsuya Suwabe Mutsuaki Edama Go Omori Noriaki Yamamoto Tomoyuki Kawase Characterization of Leukocyte- and Platelet-Rich Plasma Derived from Female Collage Athletes: A Cross-Sectional Cohort Study Focusing on Growth Factor, Inflammatory Cytokines, and Anti-Inflammatory Cytokine Levels International Journal of Molecular Sciences leukocyte- and platelet-rich plasma female college athletes PDGF-BB TGFβ1 IL-1β IL-1RA |
| title | Characterization of Leukocyte- and Platelet-Rich Plasma Derived from Female Collage Athletes: A Cross-Sectional Cohort Study Focusing on Growth Factor, Inflammatory Cytokines, and Anti-Inflammatory Cytokine Levels |
| title_full | Characterization of Leukocyte- and Platelet-Rich Plasma Derived from Female Collage Athletes: A Cross-Sectional Cohort Study Focusing on Growth Factor, Inflammatory Cytokines, and Anti-Inflammatory Cytokine Levels |
| title_fullStr | Characterization of Leukocyte- and Platelet-Rich Plasma Derived from Female Collage Athletes: A Cross-Sectional Cohort Study Focusing on Growth Factor, Inflammatory Cytokines, and Anti-Inflammatory Cytokine Levels |
| title_full_unstemmed | Characterization of Leukocyte- and Platelet-Rich Plasma Derived from Female Collage Athletes: A Cross-Sectional Cohort Study Focusing on Growth Factor, Inflammatory Cytokines, and Anti-Inflammatory Cytokine Levels |
| title_short | Characterization of Leukocyte- and Platelet-Rich Plasma Derived from Female Collage Athletes: A Cross-Sectional Cohort Study Focusing on Growth Factor, Inflammatory Cytokines, and Anti-Inflammatory Cytokine Levels |
| title_sort | characterization of leukocyte and platelet rich plasma derived from female collage athletes a cross sectional cohort study focusing on growth factor inflammatory cytokines and anti inflammatory cytokine levels |
| topic | leukocyte- and platelet-rich plasma female college athletes PDGF-BB TGFβ1 IL-1β IL-1RA |
| url | https://www.mdpi.com/1422-0067/24/17/13592 |
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