On the relationship between metabolic capacities and in vivo viscoelastic properties of the liver
The liver is the central metabolic organ. It constantly adapts its metabolic capacity to current physiological requirements. However, the relationship between tissue structure and hepatic function is incompletely understood; this results in a lack of diagnostic markers in medical imaging that can pr...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1042711/full |
| _version_ | 1797957672870346752 |
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| author | Mehrgan Shahryari Sarah Keller David Meierhofer Iwona Wallach Yasmine Safraou Jing Guo Stephan R. Marticorena Garcia Jürgen Braun Marcus R. Makowski Ingolf Sack Nikolaus Berndt |
| author_facet | Mehrgan Shahryari Sarah Keller David Meierhofer Iwona Wallach Yasmine Safraou Jing Guo Stephan R. Marticorena Garcia Jürgen Braun Marcus R. Makowski Ingolf Sack Nikolaus Berndt |
| author_sort | Mehrgan Shahryari |
| collection | DOAJ |
| description | The liver is the central metabolic organ. It constantly adapts its metabolic capacity to current physiological requirements. However, the relationship between tissue structure and hepatic function is incompletely understood; this results in a lack of diagnostic markers in medical imaging that can provide information about the liver’s metabolic capacity. Therefore, using normal rabbit livers, we combined magnetic resonance elastography (MRE) with proteomics-based kinetic modeling of central liver metabolism to investigate the potential role of MRE for predicting the liver’s metabolic function in vivo. Nineteen New Zealand white rabbits were investigated by multifrequency MRE and positron emission tomography (PET). This yielded maps of shear wave speed (SWS), penetration rate (PR) and standardized uptake value (SUV). Proteomic analysis was performed after the scans. Hepatic metabolic functions were assessed on the basis of the HEPATOKIN1 model in combination with a model of hepatic lipid-droplet metabolism using liquid chromatography–mass spectrometry. Our results showed marked differences between individual livers in both metabolic functions and stiffness properties, though not in SUV. When livers were divided into ‘stiff’ and ‘soft’ subgroups (cutoff SWS = 1.6 m/s), stiff livers showed a lower capacity for triacylglycerol storage, while at the same time showing an increased capacity for gluconeogenesis and cholesterol synthesis. Furthermore, SWS was correlated with gluconeogenesis and PR with urea production and glutamine exchange. In conclusion, our study indicates a close relationship between the viscoelastic properties of the liver and metabolic function. This could be used in future studies to predict non-invasively the functional reserve capacity of the liver in patients. |
| first_indexed | 2024-04-11T00:08:32Z |
| format | Article |
| id | doaj.art-3613f4793683453d974e6c7469aacbab |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-11T00:08:32Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-3613f4793683453d974e6c7469aacbab2023-01-09T08:05:41ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-01-011010.3389/fbioe.2022.10427111042711On the relationship between metabolic capacities and in vivo viscoelastic properties of the liverMehrgan Shahryari0Sarah Keller1David Meierhofer2Iwona Wallach3Yasmine Safraou4Jing Guo5Stephan R. Marticorena Garcia6Jürgen Braun7Marcus R. Makowski8Ingolf Sack9Nikolaus Berndt10Department of Radiology, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, GermanyDepartment of Radiology, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, GermanyMass Spectrometry Facility, Max Planck Institute for Molecular Genetics, Berlin, GermanyInstitute of Computer-Assisted Cardiovascular Medicine, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, GermanyDepartment of Radiology, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, GermanyDepartment of Radiology, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, GermanyDepartment of Radiology, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, GermanyInstitute of Medical Informatics, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, GermanyDepartment of Diagnostic and Interventional Radiology, Technical University of Munich, Faculty of Medicine, Munich, GermanyDepartment of Radiology, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, GermanyInstitute of Computer-Assisted Cardiovascular Medicine, Charité—Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, GermanyThe liver is the central metabolic organ. It constantly adapts its metabolic capacity to current physiological requirements. However, the relationship between tissue structure and hepatic function is incompletely understood; this results in a lack of diagnostic markers in medical imaging that can provide information about the liver’s metabolic capacity. Therefore, using normal rabbit livers, we combined magnetic resonance elastography (MRE) with proteomics-based kinetic modeling of central liver metabolism to investigate the potential role of MRE for predicting the liver’s metabolic function in vivo. Nineteen New Zealand white rabbits were investigated by multifrequency MRE and positron emission tomography (PET). This yielded maps of shear wave speed (SWS), penetration rate (PR) and standardized uptake value (SUV). Proteomic analysis was performed after the scans. Hepatic metabolic functions were assessed on the basis of the HEPATOKIN1 model in combination with a model of hepatic lipid-droplet metabolism using liquid chromatography–mass spectrometry. Our results showed marked differences between individual livers in both metabolic functions and stiffness properties, though not in SUV. When livers were divided into ‘stiff’ and ‘soft’ subgroups (cutoff SWS = 1.6 m/s), stiff livers showed a lower capacity for triacylglycerol storage, while at the same time showing an increased capacity for gluconeogenesis and cholesterol synthesis. Furthermore, SWS was correlated with gluconeogenesis and PR with urea production and glutamine exchange. In conclusion, our study indicates a close relationship between the viscoelastic properties of the liver and metabolic function. This could be used in future studies to predict non-invasively the functional reserve capacity of the liver in patients.https://www.frontiersin.org/articles/10.3389/fbioe.2022.1042711/fullviscoelasticityMREPETstiffnessliver metabolismproteomics |
| spellingShingle | Mehrgan Shahryari Sarah Keller David Meierhofer Iwona Wallach Yasmine Safraou Jing Guo Stephan R. Marticorena Garcia Jürgen Braun Marcus R. Makowski Ingolf Sack Nikolaus Berndt On the relationship between metabolic capacities and in vivo viscoelastic properties of the liver Frontiers in Bioengineering and Biotechnology viscoelasticity MRE PET stiffness liver metabolism proteomics |
| title | On the relationship between metabolic capacities and in vivo viscoelastic properties of the liver |
| title_full | On the relationship between metabolic capacities and in vivo viscoelastic properties of the liver |
| title_fullStr | On the relationship between metabolic capacities and in vivo viscoelastic properties of the liver |
| title_full_unstemmed | On the relationship between metabolic capacities and in vivo viscoelastic properties of the liver |
| title_short | On the relationship between metabolic capacities and in vivo viscoelastic properties of the liver |
| title_sort | on the relationship between metabolic capacities and in vivo viscoelastic properties of the liver |
| topic | viscoelasticity MRE PET stiffness liver metabolism proteomics |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1042711/full |
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