Molecular Identification of Clinical Isolates of Mycobacterium fortuitum by Random Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction and ERIC PCR
Backgrounds: Non tuberculous mycobacteria (NTM) are of importance now-a-days due to their increasing virulence outbreaks and emerging antibiotic resistance. Since the most common NTM in Iran is reportedly Mycobacterium fortuitum, the present study was designed with the aim of molecular identifi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2015-12-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/6909/15504_CE[Ra1]_F(GH)_PF1(Bm_Om)_PFA(AK)_PF2(PAG).pdf |
| Summary: | Backgrounds: Non tuberculous mycobacteria (NTM) are
of importance now-a-days due to their increasing virulence
outbreaks and emerging antibiotic resistance. Since the most
common NTM in Iran is reportedly Mycobacterium fortuitum,
the present study was designed with the aim of molecular
identification of clinical isolates of M. foruitum to analyse their
heterogeneity.
Materials and Methods: A total of 81 isolates of NTM isolated
from various samples were collected. The clinical isolates
were assigned to species M. fortuitum by using conventional
and molecular methods. The DNA banding patterns of ERICPCR and RAPD- PCR were analysed by using Bionumeric 7.5
software.
Results: Out of 81 tested NTM, 36 strains of M. fortuitum were
identified. 33 isolates were selected for molecular typing in this
study. Based on RAPD and ERIC analysis, M. fortuitum isolates
were divided into 3 and 6 clusters, respectively. Most of the
isolates were distributed into types of II RAPD (20 members/ 60.6
%) and V (14 members/ 42.4% with sub cluster I& II) of ERIC. In
RAPD analysis, the major fragments were 300 bp, followed by
fragment 1000. In ERIC analysis, the major fragments were 280
bp followed by fragment 1200 bp.
Conclusion: In conclusion, though the results from this study
represented higher discriminatory power of ERIC, however the
combination of RAPD and ERIC analysis were able to sufficiently
discriminate the genotypic diversity, infection control, and
gain useful epidemiological information regarding M. fortuitum
isolates. |
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| ISSN: | 2249-782X 0973-709X |