Molecular Identification of Clinical Isolates of Mycobacterium fortuitum by Random Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction and ERIC PCR
Backgrounds: Non tuberculous mycobacteria (NTM) are of importance now-a-days due to their increasing virulence outbreaks and emerging antibiotic resistance. Since the most common NTM in Iran is reportedly Mycobacterium fortuitum, the present study was designed with the aim of molecular identifi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2015-12-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/6909/15504_CE[Ra1]_F(GH)_PF1(Bm_Om)_PFA(AK)_PF2(PAG).pdf |
| _version_ | 1817983737954041856 |
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| author | Azar Dokht Khosravi Rasa Sheini Mehrabzadeh Abbas Farahani Hooshang Jamali |
| author_facet | Azar Dokht Khosravi Rasa Sheini Mehrabzadeh Abbas Farahani Hooshang Jamali |
| author_sort | Azar Dokht Khosravi |
| collection | DOAJ |
| description | Backgrounds: Non tuberculous mycobacteria (NTM) are
of importance now-a-days due to their increasing virulence
outbreaks and emerging antibiotic resistance. Since the most
common NTM in Iran is reportedly Mycobacterium fortuitum,
the present study was designed with the aim of molecular
identification of clinical isolates of M. foruitum to analyse their
heterogeneity.
Materials and Methods: A total of 81 isolates of NTM isolated
from various samples were collected. The clinical isolates
were assigned to species M. fortuitum by using conventional
and molecular methods. The DNA banding patterns of ERICPCR and RAPD- PCR were analysed by using Bionumeric 7.5
software.
Results: Out of 81 tested NTM, 36 strains of M. fortuitum were
identified. 33 isolates were selected for molecular typing in this
study. Based on RAPD and ERIC analysis, M. fortuitum isolates
were divided into 3 and 6 clusters, respectively. Most of the
isolates were distributed into types of II RAPD (20 members/ 60.6
%) and V (14 members/ 42.4% with sub cluster I& II) of ERIC. In
RAPD analysis, the major fragments were 300 bp, followed by
fragment 1000. In ERIC analysis, the major fragments were 280
bp followed by fragment 1200 bp.
Conclusion: In conclusion, though the results from this study
represented higher discriminatory power of ERIC, however the
combination of RAPD and ERIC analysis were able to sufficiently
discriminate the genotypic diversity, infection control, and
gain useful epidemiological information regarding M. fortuitum
isolates. |
| first_indexed | 2024-04-13T23:36:53Z |
| format | Article |
| id | doaj.art-3618bb53b28d4800bd04f9dd9184e7a8 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-04-13T23:36:53Z |
| publishDate | 2015-12-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-3618bb53b28d4800bd04f9dd9184e7a82022-12-22T02:24:42ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2015-12-01912DC01DC0510.7860/JCDR/2015/15504.6909Molecular Identification of Clinical Isolates of Mycobacterium fortuitum by Random Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction and ERIC PCRAzar Dokht Khosravi0Rasa Sheini Mehrabzadeh1Abbas Farahani2Hooshang Jamali3Professor, Department of Microbiology, School of Medicine, Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Research Assistant, Department of Microbiology, Islamic Azad University, Jahrom Branch, Iran. PhD Candidate, Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Assistant Professor, Department of Immunology, Islamic Azad University, Jahrom Branch, Iran.Backgrounds: Non tuberculous mycobacteria (NTM) are of importance now-a-days due to their increasing virulence outbreaks and emerging antibiotic resistance. Since the most common NTM in Iran is reportedly Mycobacterium fortuitum, the present study was designed with the aim of molecular identification of clinical isolates of M. foruitum to analyse their heterogeneity. Materials and Methods: A total of 81 isolates of NTM isolated from various samples were collected. The clinical isolates were assigned to species M. fortuitum by using conventional and molecular methods. The DNA banding patterns of ERICPCR and RAPD- PCR were analysed by using Bionumeric 7.5 software. Results: Out of 81 tested NTM, 36 strains of M. fortuitum were identified. 33 isolates were selected for molecular typing in this study. Based on RAPD and ERIC analysis, M. fortuitum isolates were divided into 3 and 6 clusters, respectively. Most of the isolates were distributed into types of II RAPD (20 members/ 60.6 %) and V (14 members/ 42.4% with sub cluster I& II) of ERIC. In RAPD analysis, the major fragments were 300 bp, followed by fragment 1000. In ERIC analysis, the major fragments were 280 bp followed by fragment 1200 bp. Conclusion: In conclusion, though the results from this study represented higher discriminatory power of ERIC, however the combination of RAPD and ERIC analysis were able to sufficiently discriminate the genotypic diversity, infection control, and gain useful epidemiological information regarding M. fortuitum isolates.https://jcdr.net/articles/PDF/6909/15504_CE[Ra1]_F(GH)_PF1(Bm_Om)_PFA(AK)_PF2(PAG).pdfmolecular epidemiologynon tuberculous mycobacteriatyping |
| spellingShingle | Azar Dokht Khosravi Rasa Sheini Mehrabzadeh Abbas Farahani Hooshang Jamali Molecular Identification of Clinical Isolates of Mycobacterium fortuitum by Random Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction and ERIC PCR Journal of Clinical and Diagnostic Research molecular epidemiology non tuberculous mycobacteria typing |
| title | Molecular Identification of Clinical Isolates of Mycobacterium fortuitum by Random Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction and ERIC PCR |
| title_full | Molecular Identification of Clinical Isolates of Mycobacterium fortuitum by Random Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction and ERIC PCR |
| title_fullStr | Molecular Identification of Clinical Isolates of Mycobacterium fortuitum by Random Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction and ERIC PCR |
| title_full_unstemmed | Molecular Identification of Clinical Isolates of Mycobacterium fortuitum by Random Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction and ERIC PCR |
| title_short | Molecular Identification of Clinical Isolates of Mycobacterium fortuitum by Random Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction and ERIC PCR |
| title_sort | molecular identification of clinical isolates of mycobacterium fortuitum by random amplified polymorphic dna rapd polymerase chain reaction and eric pcr |
| topic | molecular epidemiology non tuberculous mycobacteria typing |
| url | https://jcdr.net/articles/PDF/6909/15504_CE[Ra1]_F(GH)_PF1(Bm_Om)_PFA(AK)_PF2(PAG).pdf |
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