Assessment of the Influence of Size and Concentration on the Ecotoxicity of Microplastics to Microalgae <i>Scenedesmus</i> sp., Bacterium <i>Pseudomonas putida</i> and Yeast <i>Saccharomyces cerevisiae</i>
The harmful effects of microplastics are not yet fully revealed. This study tested harmful effects of polyethylene (PE), polypropylene (PP), polystyrene (PS), polyvinyl chloride (PVC), and polyethylene terephthalate (PET) microplastics were tested. Growth inhibition tests were conducted using three...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/6/1246 |
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| author | Martina Miloloža Kristina Bule Viktorija Prevarić Matija Cvetnić Šime Ukić Tomislav Bolanča Dajana Kučić Grgić |
| author_facet | Martina Miloloža Kristina Bule Viktorija Prevarić Matija Cvetnić Šime Ukić Tomislav Bolanča Dajana Kučić Grgić |
| author_sort | Martina Miloloža |
| collection | DOAJ |
| description | The harmful effects of microplastics are not yet fully revealed. This study tested harmful effects of polyethylene (PE), polypropylene (PP), polystyrene (PS), polyvinyl chloride (PVC), and polyethylene terephthalate (PET) microplastics were tested. Growth inhibition tests were conducted using three microorganisms with different characteristics: <i>Scenedesmus</i> sp., <i>Pseudomonas putida</i>, and <i>Saccharomyces cerevisiae</i>. The growth inhibition test with <i>Scenedesmus</i> sp. is relatively widely used, while the tests with <i>Pseudomonas putida</i> and <i>Saccharomyces cerevisiae</i> were, to our knowledge, applied to microplastics for the first time. The influence of concentration and size of microplastic particles, in the range of 50–1000 mg/L and 200–600 µm, was tested. Determined inhibitions on all three microorganisms confirmed the hazardous potential of the microplastics used. Modeling of the inhibition surface showed the increase in harmfulness with increasing concentration of the microplastics. Particle size showed no effect for <i>Scenedesmus</i> with PE, PP and PET, <i>Pseudomonas putida</i> with PS, and <i>Saccharomyces cerevisiae</i> with PP. In the remaining cases, higher inhibitions followed a decrease in particle size. The exception was <i>Scenedesmus</i> sp. with PS, where the lowest inhibitions were obtained at 400 µm. Finally, among the applied tests, the test with <i>Saccharomyces cerevisiae</i> proved to be the most sensitive to microplastics. |
| first_indexed | 2024-03-09T12:51:45Z |
| format | Article |
| id | doaj.art-361b125a38db4546bce298010910fbd3 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T12:51:45Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-361b125a38db4546bce298010910fbd32023-11-30T22:04:39ZengMDPI AGPolymers2073-43602022-03-01146124610.3390/polym14061246Assessment of the Influence of Size and Concentration on the Ecotoxicity of Microplastics to Microalgae <i>Scenedesmus</i> sp., Bacterium <i>Pseudomonas putida</i> and Yeast <i>Saccharomyces cerevisiae</i>Martina Miloloža0Kristina Bule1Viktorija Prevarić2Matija Cvetnić3Šime Ukić4Tomislav Bolanča5Dajana Kučić Grgić6Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, CroatiaFaculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, CroatiaFaculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, CroatiaFaculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, CroatiaFaculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, CroatiaFaculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, CroatiaFaculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, 10000 Zagreb, CroatiaThe harmful effects of microplastics are not yet fully revealed. This study tested harmful effects of polyethylene (PE), polypropylene (PP), polystyrene (PS), polyvinyl chloride (PVC), and polyethylene terephthalate (PET) microplastics were tested. Growth inhibition tests were conducted using three microorganisms with different characteristics: <i>Scenedesmus</i> sp., <i>Pseudomonas putida</i>, and <i>Saccharomyces cerevisiae</i>. The growth inhibition test with <i>Scenedesmus</i> sp. is relatively widely used, while the tests with <i>Pseudomonas putida</i> and <i>Saccharomyces cerevisiae</i> were, to our knowledge, applied to microplastics for the first time. The influence of concentration and size of microplastic particles, in the range of 50–1000 mg/L and 200–600 µm, was tested. Determined inhibitions on all three microorganisms confirmed the hazardous potential of the microplastics used. Modeling of the inhibition surface showed the increase in harmfulness with increasing concentration of the microplastics. Particle size showed no effect for <i>Scenedesmus</i> with PE, PP and PET, <i>Pseudomonas putida</i> with PS, and <i>Saccharomyces cerevisiae</i> with PP. In the remaining cases, higher inhibitions followed a decrease in particle size. The exception was <i>Scenedesmus</i> sp. with PS, where the lowest inhibitions were obtained at 400 µm. Finally, among the applied tests, the test with <i>Saccharomyces cerevisiae</i> proved to be the most sensitive to microplastics.https://www.mdpi.com/2073-4360/14/6/1246microplasticsecotoxicity assessmentsize influenceconcentration influence |
| spellingShingle | Martina Miloloža Kristina Bule Viktorija Prevarić Matija Cvetnić Šime Ukić Tomislav Bolanča Dajana Kučić Grgić Assessment of the Influence of Size and Concentration on the Ecotoxicity of Microplastics to Microalgae <i>Scenedesmus</i> sp., Bacterium <i>Pseudomonas putida</i> and Yeast <i>Saccharomyces cerevisiae</i> Polymers microplastics ecotoxicity assessment size influence concentration influence |
| title | Assessment of the Influence of Size and Concentration on the Ecotoxicity of Microplastics to Microalgae <i>Scenedesmus</i> sp., Bacterium <i>Pseudomonas putida</i> and Yeast <i>Saccharomyces cerevisiae</i> |
| title_full | Assessment of the Influence of Size and Concentration on the Ecotoxicity of Microplastics to Microalgae <i>Scenedesmus</i> sp., Bacterium <i>Pseudomonas putida</i> and Yeast <i>Saccharomyces cerevisiae</i> |
| title_fullStr | Assessment of the Influence of Size and Concentration on the Ecotoxicity of Microplastics to Microalgae <i>Scenedesmus</i> sp., Bacterium <i>Pseudomonas putida</i> and Yeast <i>Saccharomyces cerevisiae</i> |
| title_full_unstemmed | Assessment of the Influence of Size and Concentration on the Ecotoxicity of Microplastics to Microalgae <i>Scenedesmus</i> sp., Bacterium <i>Pseudomonas putida</i> and Yeast <i>Saccharomyces cerevisiae</i> |
| title_short | Assessment of the Influence of Size and Concentration on the Ecotoxicity of Microplastics to Microalgae <i>Scenedesmus</i> sp., Bacterium <i>Pseudomonas putida</i> and Yeast <i>Saccharomyces cerevisiae</i> |
| title_sort | assessment of the influence of size and concentration on the ecotoxicity of microplastics to microalgae i scenedesmus i sp bacterium i pseudomonas putida i and yeast i saccharomyces cerevisiae i |
| topic | microplastics ecotoxicity assessment size influence concentration influence |
| url | https://www.mdpi.com/2073-4360/14/6/1246 |
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