Azole selenourea disrupted the midgut and caused malformed development of Plutella xylostella
Chemical insecticides targeting the digestive system of diamondback moth (DBM), Plutella xylostella, have not been developed. The discovery of an insecticide with novel mode of action is a challenge for the control of DBM. In this study, a class of selenium- and difluoromethyl-modified azoles (fluor...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311922002088 |
| _version_ | 1797847278340276224 |
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| author | Xue-ying GUO Zi-hao HUANG Lan-tu XIONG Li DONG Yue-kun HUANG Lin-hao WEI Ri-yuan TANG Zhi-lin WANG Han-hong XU |
| author_facet | Xue-ying GUO Zi-hao HUANG Lan-tu XIONG Li DONG Yue-kun HUANG Lin-hao WEI Ri-yuan TANG Zhi-lin WANG Han-hong XU |
| author_sort | Xue-ying GUO |
| collection | DOAJ |
| description | Chemical insecticides targeting the digestive system of diamondback moth (DBM), Plutella xylostella, have not been developed. The discovery of an insecticide with novel mode of action is a challenge for the control of DBM. In this study, a class of selenium- and difluoromethyl-modified azoles (fluoroazole selenoureas, FASU) were designed and synthesized for the control of DBM. Of these azoles, compound B4 showed the highest insecticidal activity against DBM. The LC50 of third- and second-instar larvae reached 32.3 and 4.6 μg mL–1, respectively. The midgut tissue of larvae was severely disrupted, and the larval intestinal tissue was dotted with unique red spots after treatment with compound B4. Compound B4 led to disintegration of the peritrophic matrix, swelling of the midgut epithelium, fracture of the microvilli, and extensive leakage of cellular debris in the midgut lumen. Surviving larvae grew very slowly, and the larval duration was significantly prolonged after exposure to compound B4 at sublethal doses (LC10, LC25 and LC50). Furthermore, the pupation rate, emergence rate and pupae weight were significantly decreased. Compound B4 also induced abnormal pupae, causing adults to be trapped in the cocoon or failure to fly due to twisted wings. These results demonstrated that FASU could reduce the population of DBM in sublethal doses. FASU is the first synthetic insecticidal lead compound that has been shown to disrupt the midgut tissue of the larvae of DBM, and its mode of action totally differs from that of commercial chemical insecticides. |
| first_indexed | 2024-04-09T18:08:45Z |
| format | Article |
| id | doaj.art-361b56ae889c47a8ac219c6f944aea47 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-04-09T18:08:45Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-361b56ae889c47a8ac219c6f944aea472023-04-14T04:18:59ZengElsevierJournal of Integrative Agriculture2095-31192023-04-0122411041116Azole selenourea disrupted the midgut and caused malformed development of Plutella xylostellaXue-ying GUO0Zi-hao HUANG1Lan-tu XIONG2Li DONG3Yue-kun HUANG4Lin-hao WEI5Ri-yuan TANG6Zhi-lin WANG7Han-hong XU8Department of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, P.R.China; Key Laboratory of Natural Pesticide & Chemical Biology of Ministry of Education, South China Agricultural University, Guangzhou 510642, P.R.ChinaDepartment of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, P.R.ChinaDepartment of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, P.R.ChinaDepartment of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, P.R.ChinaDepartment of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, P.R.ChinaDepartment of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, P.R.ChinaDepartment of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, P.R.China; Key Laboratory of Natural Pesticide & Chemical Biology of Ministry of Education, South China Agricultural University, Guangzhou 510642, P.R.China; Correspondence TANG Riyuan, Mobile: +86-13825112030Department of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, P.R.ChinaKey Laboratory of Natural Pesticide & Chemical Biology of Ministry of Education, South China Agricultural University, Guangzhou 510642, P.R.ChinaChemical insecticides targeting the digestive system of diamondback moth (DBM), Plutella xylostella, have not been developed. The discovery of an insecticide with novel mode of action is a challenge for the control of DBM. In this study, a class of selenium- and difluoromethyl-modified azoles (fluoroazole selenoureas, FASU) were designed and synthesized for the control of DBM. Of these azoles, compound B4 showed the highest insecticidal activity against DBM. The LC50 of third- and second-instar larvae reached 32.3 and 4.6 μg mL–1, respectively. The midgut tissue of larvae was severely disrupted, and the larval intestinal tissue was dotted with unique red spots after treatment with compound B4. Compound B4 led to disintegration of the peritrophic matrix, swelling of the midgut epithelium, fracture of the microvilli, and extensive leakage of cellular debris in the midgut lumen. Surviving larvae grew very slowly, and the larval duration was significantly prolonged after exposure to compound B4 at sublethal doses (LC10, LC25 and LC50). Furthermore, the pupation rate, emergence rate and pupae weight were significantly decreased. Compound B4 also induced abnormal pupae, causing adults to be trapped in the cocoon or failure to fly due to twisted wings. These results demonstrated that FASU could reduce the population of DBM in sublethal doses. FASU is the first synthetic insecticidal lead compound that has been shown to disrupt the midgut tissue of the larvae of DBM, and its mode of action totally differs from that of commercial chemical insecticides.http://www.sciencedirect.com/science/article/pii/S2095311922002088Plutella xylostellaazoleseleniumdigestive systemmidgut |
| spellingShingle | Xue-ying GUO Zi-hao HUANG Lan-tu XIONG Li DONG Yue-kun HUANG Lin-hao WEI Ri-yuan TANG Zhi-lin WANG Han-hong XU Azole selenourea disrupted the midgut and caused malformed development of Plutella xylostella Journal of Integrative Agriculture Plutella xylostella azole selenium digestive system midgut |
| title | Azole selenourea disrupted the midgut and caused malformed development of Plutella xylostella |
| title_full | Azole selenourea disrupted the midgut and caused malformed development of Plutella xylostella |
| title_fullStr | Azole selenourea disrupted the midgut and caused malformed development of Plutella xylostella |
| title_full_unstemmed | Azole selenourea disrupted the midgut and caused malformed development of Plutella xylostella |
| title_short | Azole selenourea disrupted the midgut and caused malformed development of Plutella xylostella |
| title_sort | azole selenourea disrupted the midgut and caused malformed development of plutella xylostella |
| topic | Plutella xylostella azole selenium digestive system midgut |
| url | http://www.sciencedirect.com/science/article/pii/S2095311922002088 |
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