Identification of <i>Contracaecum rudolphii</i> (Nematoda: Anisakidae) in Great Cormorants <i>Phalacrocorax carbo sinensis</i> (Blumenbach, 1978) from Southern Italy
In this study, four dead great cormorant <i>Phalacrocorax carbo sinensis</i> (Blumenbach, 1978) specimens, collected from the coasts and lakes of Southern Italy, were examined by necropsy for the detection of <i>Contraceacum</i> sp. The adults and larvae found were subjected...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
|
| Series: | Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-7381/10/3/194 |
| _version_ | 1797608672866598912 |
|---|---|
| author | Gaetano Cammilleri Stefano D’Amelio Vincenzo Ferrantelli Antonella Costa Maria Drussilla Buscemi Annamaria Castello Emanuela Bacchi Elisa Goffredo Maria Emanuela Mancini Serena Cavallero |
| author_facet | Gaetano Cammilleri Stefano D’Amelio Vincenzo Ferrantelli Antonella Costa Maria Drussilla Buscemi Annamaria Castello Emanuela Bacchi Elisa Goffredo Maria Emanuela Mancini Serena Cavallero |
| author_sort | Gaetano Cammilleri |
| collection | DOAJ |
| description | In this study, four dead great cormorant <i>Phalacrocorax carbo sinensis</i> (Blumenbach, 1978) specimens, collected from the coasts and lakes of Southern Italy, were examined by necropsy for the detection of <i>Contraceacum</i> sp. The adults and larvae found were subjected to morphological analysis and molecular identification by PCR-RFLP. A total of 181 <i>Contracaecum</i> specimens were detected in all of the four great cormorants examined (prevalence = 100%), showing an intensity of infestation between nine and ninety-two. A co-infestation by adult and larval forms of <i>Contracaecum rudolphii</i> was found only in one of the great cormorants examined. Following molecular investigations, 48 specimens of <i>C. rudolphii</i> A and 38 specimens of <i>C. rudolphii</i> B were detected, revealing co-infestation solely for the great cormorant from Leporano Bay (Southern Italy). Our results showed an opposite ratio between <i>C. rudolphii</i> A and <i>C. rudolphii</i> B in Pantelleria and in Salso Lake (Southern Italy) compared to what was reported in the literature, probably due to migratory stopovers and the ecology of the infested fish species, confirming the role of <i>Contracaecum</i> nematodes as ecological tags of their hosts. |
| first_indexed | 2024-03-11T05:46:52Z |
| format | Article |
| id | doaj.art-361c050531ec4612b1b7c25b4fe941c1 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-11T05:46:52Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-361c050531ec4612b1b7c25b4fe941c12023-11-17T14:20:36ZengMDPI AGVeterinary Sciences2306-73812023-03-0110319410.3390/vetsci10030194Identification of <i>Contracaecum rudolphii</i> (Nematoda: Anisakidae) in Great Cormorants <i>Phalacrocorax carbo sinensis</i> (Blumenbach, 1978) from Southern ItalyGaetano Cammilleri0Stefano D’Amelio1Vincenzo Ferrantelli2Antonella Costa3Maria Drussilla Buscemi4Annamaria Castello5Emanuela Bacchi6Elisa Goffredo7Maria Emanuela Mancini8Serena Cavallero9Centro di Referenza Nazionale per le Anisakiasi, Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via Gino Marinuzzi 3, 90129 Palermo, ItalyDepartment of Public Health and Infectious Diseases, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyCentro di Referenza Nazionale per le Anisakiasi, Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via Gino Marinuzzi 3, 90129 Palermo, ItalyCentro di Referenza Nazionale per le Anisakiasi, Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via Gino Marinuzzi 3, 90129 Palermo, ItalyCentro di Referenza Nazionale per le Anisakiasi, Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via Gino Marinuzzi 3, 90129 Palermo, ItalyCentro di Referenza Nazionale per le Anisakiasi, Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via Gino Marinuzzi 3, 90129 Palermo, ItalyCentro di Referenza Nazionale per le Anisakiasi, Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via Gino Marinuzzi 3, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, ItalyIstituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, ItalyDepartment of Public Health and Infectious Diseases, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyIn this study, four dead great cormorant <i>Phalacrocorax carbo sinensis</i> (Blumenbach, 1978) specimens, collected from the coasts and lakes of Southern Italy, were examined by necropsy for the detection of <i>Contraceacum</i> sp. The adults and larvae found were subjected to morphological analysis and molecular identification by PCR-RFLP. A total of 181 <i>Contracaecum</i> specimens were detected in all of the four great cormorants examined (prevalence = 100%), showing an intensity of infestation between nine and ninety-two. A co-infestation by adult and larval forms of <i>Contracaecum rudolphii</i> was found only in one of the great cormorants examined. Following molecular investigations, 48 specimens of <i>C. rudolphii</i> A and 38 specimens of <i>C. rudolphii</i> B were detected, revealing co-infestation solely for the great cormorant from Leporano Bay (Southern Italy). Our results showed an opposite ratio between <i>C. rudolphii</i> A and <i>C. rudolphii</i> B in Pantelleria and in Salso Lake (Southern Italy) compared to what was reported in the literature, probably due to migratory stopovers and the ecology of the infested fish species, confirming the role of <i>Contracaecum</i> nematodes as ecological tags of their hosts.https://www.mdpi.com/2306-7381/10/3/194<i>Anisakidae</i><i>Contracaecum</i>PCR-RFLPSouth Mediterranean |
| spellingShingle | Gaetano Cammilleri Stefano D’Amelio Vincenzo Ferrantelli Antonella Costa Maria Drussilla Buscemi Annamaria Castello Emanuela Bacchi Elisa Goffredo Maria Emanuela Mancini Serena Cavallero Identification of <i>Contracaecum rudolphii</i> (Nematoda: Anisakidae) in Great Cormorants <i>Phalacrocorax carbo sinensis</i> (Blumenbach, 1978) from Southern Italy Veterinary Sciences <i>Anisakidae</i> <i>Contracaecum</i> PCR-RFLP South Mediterranean |
| title | Identification of <i>Contracaecum rudolphii</i> (Nematoda: Anisakidae) in Great Cormorants <i>Phalacrocorax carbo sinensis</i> (Blumenbach, 1978) from Southern Italy |
| title_full | Identification of <i>Contracaecum rudolphii</i> (Nematoda: Anisakidae) in Great Cormorants <i>Phalacrocorax carbo sinensis</i> (Blumenbach, 1978) from Southern Italy |
| title_fullStr | Identification of <i>Contracaecum rudolphii</i> (Nematoda: Anisakidae) in Great Cormorants <i>Phalacrocorax carbo sinensis</i> (Blumenbach, 1978) from Southern Italy |
| title_full_unstemmed | Identification of <i>Contracaecum rudolphii</i> (Nematoda: Anisakidae) in Great Cormorants <i>Phalacrocorax carbo sinensis</i> (Blumenbach, 1978) from Southern Italy |
| title_short | Identification of <i>Contracaecum rudolphii</i> (Nematoda: Anisakidae) in Great Cormorants <i>Phalacrocorax carbo sinensis</i> (Blumenbach, 1978) from Southern Italy |
| title_sort | identification of i contracaecum rudolphii i nematoda anisakidae in great cormorants i phalacrocorax carbo sinensis i blumenbach 1978 from southern italy |
| topic | <i>Anisakidae</i> <i>Contracaecum</i> PCR-RFLP South Mediterranean |
| url | https://www.mdpi.com/2306-7381/10/3/194 |
| work_keys_str_mv | AT gaetanocammilleri identificationoficontracaecumrudolphiiinematodaanisakidaeingreatcormorantsiphalacrocoraxcarbosinensisiblumenbach1978fromsouthernitaly AT stefanodamelio identificationoficontracaecumrudolphiiinematodaanisakidaeingreatcormorantsiphalacrocoraxcarbosinensisiblumenbach1978fromsouthernitaly AT vincenzoferrantelli identificationoficontracaecumrudolphiiinematodaanisakidaeingreatcormorantsiphalacrocoraxcarbosinensisiblumenbach1978fromsouthernitaly AT antonellacosta identificationoficontracaecumrudolphiiinematodaanisakidaeingreatcormorantsiphalacrocoraxcarbosinensisiblumenbach1978fromsouthernitaly AT mariadrussillabuscemi identificationoficontracaecumrudolphiiinematodaanisakidaeingreatcormorantsiphalacrocoraxcarbosinensisiblumenbach1978fromsouthernitaly AT annamariacastello identificationoficontracaecumrudolphiiinematodaanisakidaeingreatcormorantsiphalacrocoraxcarbosinensisiblumenbach1978fromsouthernitaly AT emanuelabacchi identificationoficontracaecumrudolphiiinematodaanisakidaeingreatcormorantsiphalacrocoraxcarbosinensisiblumenbach1978fromsouthernitaly AT elisagoffredo identificationoficontracaecumrudolphiiinematodaanisakidaeingreatcormorantsiphalacrocoraxcarbosinensisiblumenbach1978fromsouthernitaly AT mariaemanuelamancini identificationoficontracaecumrudolphiiinematodaanisakidaeingreatcormorantsiphalacrocoraxcarbosinensisiblumenbach1978fromsouthernitaly AT serenacavallero identificationoficontracaecumrudolphiiinematodaanisakidaeingreatcormorantsiphalacrocoraxcarbosinensisiblumenbach1978fromsouthernitaly |