Starch accumulation dynamics and transcriptome analysis of Chlorella sorokiniana during transition of sulfur nutritional status
Microalgae can effectively accumulate starch by using nutritional limitation methods in the context of bioalcohol fuel production. However, relatively few studies have focused on starch accumulation in microalgae and its molecular basis, especially under sulfur limitation conditions. In this study,...
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.986400/full |
| _version_ | 1818516553906257920 |
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| author | Haiqing Xu Jinzhi Yang Xu Wang Qing Peng Yanxia Han Xudong Liu Kexin Liu Shijuan Dou Shijuan Dou Liyun Li Liyun Li Guozhen Liu Guozhen Liu Guozhen Liu Ming Yang Ming Yang Ming Yang |
| author_facet | Haiqing Xu Jinzhi Yang Xu Wang Qing Peng Yanxia Han Xudong Liu Kexin Liu Shijuan Dou Shijuan Dou Liyun Li Liyun Li Guozhen Liu Guozhen Liu Guozhen Liu Ming Yang Ming Yang Ming Yang |
| author_sort | Haiqing Xu |
| collection | DOAJ |
| description | Microalgae can effectively accumulate starch by using nutritional limitation methods in the context of bioalcohol fuel production. However, relatively few studies have focused on starch accumulation in microalgae and its molecular basis, especially under sulfur limitation conditions. In this study, the starch accumulation dynamics and physiological responses of Chlorella sorokiniana under sulfur starvation (SS) and sulfur replenishment (SR) conditions were investigated, and the genes involved in the transcriptional regulation were explored using RNA-seq. The starch content in C. sorokiniana cells significantly increased from 1.6% to 55.0% of dry weight within 24 h under SS conditions, and then, it decreased to 3.4% within 12 h after transition to SR conditions. However, cell growth was inhibited, and pigment content decreased under SS conditions. Using RNA-seq analysis, a total of 9720 differentially expressed genes (DEGs) induced by sulfur status were obtained. These genes were narrowed down to 454 starvation and replenishment cross-validated (SRV)-DEGs, among which 283 SRV-DEGs were significantly up-regulated and 171 SRV-DEGs were down-regulated under SS conditions, and returned to their previous state under SR conditions. The SRV-DEGs enriched in the sulfate metabolism pathway were all up-regulated under SS conditions after 6 h to speed up the sulfur metabolic cycle, and the transcriptional abundance of a sulfate transporter (SULTR4), cysteine synthase[O-acetylserine(thiol)-lyase] (OASTL), serine acetyltransferase (SAT), and methanethiol oxidase (SELENBP1) increased 8.6-fold, 12.6-fold, 8.7-fold, and 12.4-fold, respectively. Protein synthesis was correspondingly inhibited, which resulted in the reallocation of carbon and elevated the starch synthesis pathway, in which the expressions of glycogen branching enzyme (GBE) and starch synthase (SS) were up-regulated 12.0- and 3.0-fold, respectively. The fatty acid desaturase (FAD) and phosphatidic acid phosphatase (PAP) in the lipid synthesis pathway were strongly up-regulated 8.8- and 16.2-fold, respectively, indicating the competitive synthesis of lipids. The down-regulation of SRV-DEGs associated with carbon fixation, such as those in the Calvin cycle, possibly affected cell growth. The time-resolved transcriptional analysis identified the SRV-DEGs, revealing the underlying starch accumulation mechanism, as well as the relationship with cell growth and lipid synthesis. |
| first_indexed | 2024-12-11T00:43:52Z |
| format | Article |
| id | doaj.art-361c466684a746a4be5801de4173c4dc |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-12-11T00:43:52Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Marine Science |
| spelling | doaj.art-361c466684a746a4be5801de4173c4dc2022-12-22T01:26:50ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-08-01910.3389/fmars.2022.986400986400Starch accumulation dynamics and transcriptome analysis of Chlorella sorokiniana during transition of sulfur nutritional statusHaiqing Xu0Jinzhi Yang1Xu Wang2Qing Peng3Yanxia Han4Xudong Liu5Kexin Liu6Shijuan Dou7Shijuan Dou8Liyun Li9Liyun Li10Guozhen Liu11Guozhen Liu12Guozhen Liu13Ming Yang14Ming Yang15Ming Yang16College of Life Sciences, Hebei Agricultural University, Baoding, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding, ChinaHebei Key Laboratory of Plant Physiology and Molecular Pathology, Baoding, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding, ChinaHebei Key Laboratory of Plant Physiology and Molecular Pathology, Baoding, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding, ChinaHebei Key Laboratory of Plant Physiology and Molecular Pathology, Baoding, ChinaHebei Engineering Research Center for Agricultural Waste Resource Utilization, Baoding, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding, ChinaHebei Key Laboratory of Plant Physiology and Molecular Pathology, Baoding, ChinaHebei Engineering Research Center for Agricultural Waste Resource Utilization, Baoding, ChinaMicroalgae can effectively accumulate starch by using nutritional limitation methods in the context of bioalcohol fuel production. However, relatively few studies have focused on starch accumulation in microalgae and its molecular basis, especially under sulfur limitation conditions. In this study, the starch accumulation dynamics and physiological responses of Chlorella sorokiniana under sulfur starvation (SS) and sulfur replenishment (SR) conditions were investigated, and the genes involved in the transcriptional regulation were explored using RNA-seq. The starch content in C. sorokiniana cells significantly increased from 1.6% to 55.0% of dry weight within 24 h under SS conditions, and then, it decreased to 3.4% within 12 h after transition to SR conditions. However, cell growth was inhibited, and pigment content decreased under SS conditions. Using RNA-seq analysis, a total of 9720 differentially expressed genes (DEGs) induced by sulfur status were obtained. These genes were narrowed down to 454 starvation and replenishment cross-validated (SRV)-DEGs, among which 283 SRV-DEGs were significantly up-regulated and 171 SRV-DEGs were down-regulated under SS conditions, and returned to their previous state under SR conditions. The SRV-DEGs enriched in the sulfate metabolism pathway were all up-regulated under SS conditions after 6 h to speed up the sulfur metabolic cycle, and the transcriptional abundance of a sulfate transporter (SULTR4), cysteine synthase[O-acetylserine(thiol)-lyase] (OASTL), serine acetyltransferase (SAT), and methanethiol oxidase (SELENBP1) increased 8.6-fold, 12.6-fold, 8.7-fold, and 12.4-fold, respectively. Protein synthesis was correspondingly inhibited, which resulted in the reallocation of carbon and elevated the starch synthesis pathway, in which the expressions of glycogen branching enzyme (GBE) and starch synthase (SS) were up-regulated 12.0- and 3.0-fold, respectively. The fatty acid desaturase (FAD) and phosphatidic acid phosphatase (PAP) in the lipid synthesis pathway were strongly up-regulated 8.8- and 16.2-fold, respectively, indicating the competitive synthesis of lipids. The down-regulation of SRV-DEGs associated with carbon fixation, such as those in the Calvin cycle, possibly affected cell growth. The time-resolved transcriptional analysis identified the SRV-DEGs, revealing the underlying starch accumulation mechanism, as well as the relationship with cell growth and lipid synthesis.https://www.frontiersin.org/articles/10.3389/fmars.2022.986400/fullChlorella sorokinianasulfur-starvationsulfur-replenishmenttranscriptomic dynamicsstarch metabolism |
| spellingShingle | Haiqing Xu Jinzhi Yang Xu Wang Qing Peng Yanxia Han Xudong Liu Kexin Liu Shijuan Dou Shijuan Dou Liyun Li Liyun Li Guozhen Liu Guozhen Liu Guozhen Liu Ming Yang Ming Yang Ming Yang Starch accumulation dynamics and transcriptome analysis of Chlorella sorokiniana during transition of sulfur nutritional status Frontiers in Marine Science Chlorella sorokiniana sulfur-starvation sulfur-replenishment transcriptomic dynamics starch metabolism |
| title | Starch accumulation dynamics and transcriptome analysis of Chlorella sorokiniana during transition of sulfur nutritional status |
| title_full | Starch accumulation dynamics and transcriptome analysis of Chlorella sorokiniana during transition of sulfur nutritional status |
| title_fullStr | Starch accumulation dynamics and transcriptome analysis of Chlorella sorokiniana during transition of sulfur nutritional status |
| title_full_unstemmed | Starch accumulation dynamics and transcriptome analysis of Chlorella sorokiniana during transition of sulfur nutritional status |
| title_short | Starch accumulation dynamics and transcriptome analysis of Chlorella sorokiniana during transition of sulfur nutritional status |
| title_sort | starch accumulation dynamics and transcriptome analysis of chlorella sorokiniana during transition of sulfur nutritional status |
| topic | Chlorella sorokiniana sulfur-starvation sulfur-replenishment transcriptomic dynamics starch metabolism |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.986400/full |
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