Aptamer-Based Detection of Ampicillin in Urine Samples
The misuse of antibiotics in health care has led to increasing levels of drug resistant infections (DRI’s) occurring in the general population. Most technologies developed for the detection of DRI’s typically focus on phenotyping or genotyping bacterial resistance rather than on the underlying cause...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/9/10/655 |
| _version_ | 1827705182804246528 |
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| author | Matthew D. Simmons Lisa M. Miller Malin O. Sundström Steven Johnson |
| author_facet | Matthew D. Simmons Lisa M. Miller Malin O. Sundström Steven Johnson |
| author_sort | Matthew D. Simmons |
| collection | DOAJ |
| description | The misuse of antibiotics in health care has led to increasing levels of drug resistant infections (DRI’s) occurring in the general population. Most technologies developed for the detection of DRI’s typically focus on phenotyping or genotyping bacterial resistance rather than on the underlying cause and spread of DRI’s; namely the misuse of antibiotics. An aptameric based assay has been developed for the monitoring of ampicillin in urine samples, for use in determining optimal antibiotic dosage and monitoring patient compliance with treatment. The fluorescently labelled aptamers were shown to perform optimally at pH 7, ideal for buffered clinical urine samples, with limits of detection as low as 20.6 nM, allowing for determination of ampicillin in urine in the clinically relevant range of concentrations (100 nM to 100 µM). As the assay requires incubation for only 1 h with a small sample volume, 50 to 150 µL, the test would fit within current healthcare pathways, simplifying the adoption of the technology. |
| first_indexed | 2024-03-10T15:57:39Z |
| format | Article |
| id | doaj.art-361d4c049eb04474aea68673e59a313b |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T15:57:39Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-361d4c049eb04474aea68673e59a313b2023-11-20T15:33:04ZengMDPI AGAntibiotics2079-63822020-09-0191065510.3390/antibiotics9100655Aptamer-Based Detection of Ampicillin in Urine SamplesMatthew D. Simmons0Lisa M. Miller1Malin O. Sundström2Steven Johnson3Department of Electronic Engineering, University of York, Heslington, York, North Yorkshire YO10 5DD, UKDepartment of Chemistry, University of York, Heslington, York, North Yorkshire YO10 5DD, UKDepartment of Electronic Engineering, University of York, Heslington, York, North Yorkshire YO10 5DD, UKDepartment of Electronic Engineering, University of York, Heslington, York, North Yorkshire YO10 5DD, UKThe misuse of antibiotics in health care has led to increasing levels of drug resistant infections (DRI’s) occurring in the general population. Most technologies developed for the detection of DRI’s typically focus on phenotyping or genotyping bacterial resistance rather than on the underlying cause and spread of DRI’s; namely the misuse of antibiotics. An aptameric based assay has been developed for the monitoring of ampicillin in urine samples, for use in determining optimal antibiotic dosage and monitoring patient compliance with treatment. The fluorescently labelled aptamers were shown to perform optimally at pH 7, ideal for buffered clinical urine samples, with limits of detection as low as 20.6 nM, allowing for determination of ampicillin in urine in the clinically relevant range of concentrations (100 nM to 100 µM). As the assay requires incubation for only 1 h with a small sample volume, 50 to 150 µL, the test would fit within current healthcare pathways, simplifying the adoption of the technology.https://www.mdpi.com/2079-6382/9/10/655ampicillinaptamerdrug-resistant-infectionsantimicrobial resistancediagnosticsgold nanoparticles |
| spellingShingle | Matthew D. Simmons Lisa M. Miller Malin O. Sundström Steven Johnson Aptamer-Based Detection of Ampicillin in Urine Samples Antibiotics ampicillin aptamer drug-resistant-infections antimicrobial resistance diagnostics gold nanoparticles |
| title | Aptamer-Based Detection of Ampicillin in Urine Samples |
| title_full | Aptamer-Based Detection of Ampicillin in Urine Samples |
| title_fullStr | Aptamer-Based Detection of Ampicillin in Urine Samples |
| title_full_unstemmed | Aptamer-Based Detection of Ampicillin in Urine Samples |
| title_short | Aptamer-Based Detection of Ampicillin in Urine Samples |
| title_sort | aptamer based detection of ampicillin in urine samples |
| topic | ampicillin aptamer drug-resistant-infections antimicrobial resistance diagnostics gold nanoparticles |
| url | https://www.mdpi.com/2079-6382/9/10/655 |
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