Concentrated Growth Factors Promote hBMSCs Osteogenic Differentiation in a Co-Culture System With HUVECs
Osteogenesis is a complex physiologic process that occurs during bone regeneration. This process requires several growth factors that act on bone marrow-derived mesenchymal stem cells (BMSCs). Concentrated growth factor (CGF) is a new-generation platelet-rich derivative that is an appealing autologo...
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| Format: | Article |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.837295/full |
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| author | Yunyang Liao Yunyang Liao Yunyang Liao Youran Fang Hanghang Zhu Yue Huang Yue Huang Gengsen Zou Gengsen Zou Bowen Dai Bowen Dai Macro Aoqi Rausch Bin Shi Bin Shi Bin Shi |
| author_facet | Yunyang Liao Yunyang Liao Yunyang Liao Youran Fang Hanghang Zhu Yue Huang Yue Huang Gengsen Zou Gengsen Zou Bowen Dai Bowen Dai Macro Aoqi Rausch Bin Shi Bin Shi Bin Shi |
| author_sort | Yunyang Liao |
| collection | DOAJ |
| description | Osteogenesis is a complex physiologic process that occurs during bone regeneration. This process requires several growth factors that act on bone marrow-derived mesenchymal stem cells (BMSCs). Concentrated growth factor (CGF) is a new-generation platelet-rich derivative that is an appealing autologous material for application in tissue repair and bone regenerative medicine because it contains a variety of fibrin and growth factors. In this study, the effects of CGF on the proliferation and osteogenic differentiation of hBMSCs and human umbilical vein endothelial cells (HUVECs) were explored with in vitro cell co-culture experiments. HBMSCs and HUVECs were directly co-cultured at the ratio of 1:2 under different concentrations (0, 2, 5, 10, 20%) of CGF for 7 days. Alkaline phosphatase (ALP) staining and quantitative reverse transcription polymerase chain reaction were used to detect the effects of CGF on the expression of osteogenic genes (ALP, osteocalcin [OCN], type I collagen [COL-1], Runt-related transcription factor 2 [RUNX2]) and connexin 43 (CX43). RNA sequencing was used to explore potential regulated genes and signaling pathways that affect the osteogenesis of co-cultured hBMSCs exposed to CGF. The results showed higher expressions of ALP, COL-1, RUNX2, OCN, and CX43 in the direct co-culture group containing 10% CGF compared to the direct co-culture group without CGF and the indirect co-culture group. In summary, 10% CGF can significantly promote osteogenesis in hBMSCs directly co-cultured with HUVECs. Intercellular communication between the direct co-culture of hBMSCs and HUVECs through CX43 may be one of the main regulatory mechanisms. |
| first_indexed | 2024-12-13T19:56:00Z |
| format | Article |
| id | doaj.art-3621d718b3584c93a0d42fdc9c378423 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-13T19:56:00Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-3621d718b3584c93a0d42fdc9c3784232022-12-21T23:33:19ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-03-011010.3389/fbioe.2022.837295837295Concentrated Growth Factors Promote hBMSCs Osteogenic Differentiation in a Co-Culture System With HUVECsYunyang Liao0Yunyang Liao1Yunyang Liao2Youran Fang3Hanghang Zhu4Yue Huang5Yue Huang6Gengsen Zou7Gengsen Zou8Bowen Dai9Bowen Dai10Macro Aoqi Rausch11Bin Shi12Bin Shi13Bin Shi14Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Fujian Medical University, Fuzhou, ChinaLaboratory of Facial Plastic and Reconstruction, Fujian Medical University, Fuzhou, ChinaFujian Key Laboratory of Oral Diseases, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, ChinaFujian Key Laboratory of Oral Diseases, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, ChinaFujian Key Laboratory of Oral Diseases, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, ChinaDepartment of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Fujian Medical University, Fuzhou, ChinaLaboratory of Facial Plastic and Reconstruction, Fujian Medical University, Fuzhou, ChinaDepartment of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Fujian Medical University, Fuzhou, ChinaLaboratory of Facial Plastic and Reconstruction, Fujian Medical University, Fuzhou, ChinaDepartment of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Fujian Medical University, Fuzhou, ChinaLaboratory of Facial Plastic and Reconstruction, Fujian Medical University, Fuzhou, ChinaDivision of Orthodontics, University Clinic of Dentistry, Medical University of Vienna, Vienna, AustriaDepartment of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Fujian Medical University, Fuzhou, ChinaLaboratory of Facial Plastic and Reconstruction, Fujian Medical University, Fuzhou, ChinaFujian Key Laboratory of Oral Diseases, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, ChinaOsteogenesis is a complex physiologic process that occurs during bone regeneration. This process requires several growth factors that act on bone marrow-derived mesenchymal stem cells (BMSCs). Concentrated growth factor (CGF) is a new-generation platelet-rich derivative that is an appealing autologous material for application in tissue repair and bone regenerative medicine because it contains a variety of fibrin and growth factors. In this study, the effects of CGF on the proliferation and osteogenic differentiation of hBMSCs and human umbilical vein endothelial cells (HUVECs) were explored with in vitro cell co-culture experiments. HBMSCs and HUVECs were directly co-cultured at the ratio of 1:2 under different concentrations (0, 2, 5, 10, 20%) of CGF for 7 days. Alkaline phosphatase (ALP) staining and quantitative reverse transcription polymerase chain reaction were used to detect the effects of CGF on the expression of osteogenic genes (ALP, osteocalcin [OCN], type I collagen [COL-1], Runt-related transcription factor 2 [RUNX2]) and connexin 43 (CX43). RNA sequencing was used to explore potential regulated genes and signaling pathways that affect the osteogenesis of co-cultured hBMSCs exposed to CGF. The results showed higher expressions of ALP, COL-1, RUNX2, OCN, and CX43 in the direct co-culture group containing 10% CGF compared to the direct co-culture group without CGF and the indirect co-culture group. In summary, 10% CGF can significantly promote osteogenesis in hBMSCs directly co-cultured with HUVECs. Intercellular communication between the direct co-culture of hBMSCs and HUVECs through CX43 may be one of the main regulatory mechanisms.https://www.frontiersin.org/articles/10.3389/fbioe.2022.837295/fullConcentrated Growth Factors (CGF)cell co-culturehBMSCsosteogenic differentiationconnexin 43 (cx43) |
| spellingShingle | Yunyang Liao Yunyang Liao Yunyang Liao Youran Fang Hanghang Zhu Yue Huang Yue Huang Gengsen Zou Gengsen Zou Bowen Dai Bowen Dai Macro Aoqi Rausch Bin Shi Bin Shi Bin Shi Concentrated Growth Factors Promote hBMSCs Osteogenic Differentiation in a Co-Culture System With HUVECs Frontiers in Bioengineering and Biotechnology Concentrated Growth Factors (CGF) cell co-culture hBMSCs osteogenic differentiation connexin 43 (cx43) |
| title | Concentrated Growth Factors Promote hBMSCs Osteogenic Differentiation in a Co-Culture System With HUVECs |
| title_full | Concentrated Growth Factors Promote hBMSCs Osteogenic Differentiation in a Co-Culture System With HUVECs |
| title_fullStr | Concentrated Growth Factors Promote hBMSCs Osteogenic Differentiation in a Co-Culture System With HUVECs |
| title_full_unstemmed | Concentrated Growth Factors Promote hBMSCs Osteogenic Differentiation in a Co-Culture System With HUVECs |
| title_short | Concentrated Growth Factors Promote hBMSCs Osteogenic Differentiation in a Co-Culture System With HUVECs |
| title_sort | concentrated growth factors promote hbmscs osteogenic differentiation in a co culture system with huvecs |
| topic | Concentrated Growth Factors (CGF) cell co-culture hBMSCs osteogenic differentiation connexin 43 (cx43) |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.837295/full |
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