A Genetic Toolkit for Dissecting Dopamine Circuit Function in Drosophila
Summary: The neuromodulator dopamine (DA) plays a key role in motor control, motivated behaviors, and higher-order cognitive processes. Dissecting how these DA neural networks tune the activity of local neural circuits to regulate behavior requires tools for manipulating small groups of DA neurons....
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018-04-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124718304297 |
| _version_ | 1818200133380079616 |
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| author | Tingting Xie Margaret C.W. Ho Qili Liu Wakako Horiuchi Chun-Chieh Lin Darya Task Haojiang Luan Benjamin H. White Christopher J. Potter Mark N. Wu |
| author_facet | Tingting Xie Margaret C.W. Ho Qili Liu Wakako Horiuchi Chun-Chieh Lin Darya Task Haojiang Luan Benjamin H. White Christopher J. Potter Mark N. Wu |
| author_sort | Tingting Xie |
| collection | DOAJ |
| description | Summary: The neuromodulator dopamine (DA) plays a key role in motor control, motivated behaviors, and higher-order cognitive processes. Dissecting how these DA neural networks tune the activity of local neural circuits to regulate behavior requires tools for manipulating small groups of DA neurons. To address this need, we assembled a genetic toolkit that allows for an exquisite level of control over the DA neural network in Drosophila. To further refine targeting of specific DA neurons, we also created reagents that allow for the conversion of any existing GAL4 line into Split GAL4 or GAL80 lines. We demonstrated how this toolkit can be used with recently developed computational methods to rapidly generate additional reagents for manipulating small subsets or individual DA neurons. Finally, we used the toolkit to reveal a dynamic interaction between a small subset of DA neurons and rearing conditions in a social space behavioral assay. : The rapid analysis of how dopaminergic circuits regulate behavior is limited by the genetic tools available to target and manipulate small numbers of these neurons. Xie et al. present genetic tools in Drosophila that allow rational targeting of sparse dopaminergic neuronal subsets and selective knockdown of dopamine signaling. Keywords: dopamine, genetics, behavior, neural circuits, neuromodulation, Drosophila |
| first_indexed | 2024-12-12T02:32:49Z |
| format | Article |
| id | doaj.art-3622c75b05fe4ea5877b1ba0a13b1ab3 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-12T02:32:49Z |
| publishDate | 2018-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-3622c75b05fe4ea5877b1ba0a13b1ab32022-12-22T00:41:21ZengElsevierCell Reports2211-12472018-04-01232652665A Genetic Toolkit for Dissecting Dopamine Circuit Function in DrosophilaTingting Xie0Margaret C.W. Ho1Qili Liu2Wakako Horiuchi3Chun-Chieh Lin4Darya Task5Haojiang Luan6Benjamin H. White7Christopher J. Potter8Mark N. Wu9School of Life Sciences, Peking University, Beijing 100871, China; Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USADepartment of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USADepartment of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USADepartment of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USASolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USASolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USALaboratory of Molecular Biology, National Institute of Mental Health, NIH, Bethesda, MD 20892, USALaboratory of Molecular Biology, National Institute of Mental Health, NIH, Bethesda, MD 20892, USASolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USADepartment of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA; Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USASummary: The neuromodulator dopamine (DA) plays a key role in motor control, motivated behaviors, and higher-order cognitive processes. Dissecting how these DA neural networks tune the activity of local neural circuits to regulate behavior requires tools for manipulating small groups of DA neurons. To address this need, we assembled a genetic toolkit that allows for an exquisite level of control over the DA neural network in Drosophila. To further refine targeting of specific DA neurons, we also created reagents that allow for the conversion of any existing GAL4 line into Split GAL4 or GAL80 lines. We demonstrated how this toolkit can be used with recently developed computational methods to rapidly generate additional reagents for manipulating small subsets or individual DA neurons. Finally, we used the toolkit to reveal a dynamic interaction between a small subset of DA neurons and rearing conditions in a social space behavioral assay. : The rapid analysis of how dopaminergic circuits regulate behavior is limited by the genetic tools available to target and manipulate small numbers of these neurons. Xie et al. present genetic tools in Drosophila that allow rational targeting of sparse dopaminergic neuronal subsets and selective knockdown of dopamine signaling. Keywords: dopamine, genetics, behavior, neural circuits, neuromodulation, Drosophilahttp://www.sciencedirect.com/science/article/pii/S2211124718304297 |
| spellingShingle | Tingting Xie Margaret C.W. Ho Qili Liu Wakako Horiuchi Chun-Chieh Lin Darya Task Haojiang Luan Benjamin H. White Christopher J. Potter Mark N. Wu A Genetic Toolkit for Dissecting Dopamine Circuit Function in Drosophila Cell Reports |
| title | A Genetic Toolkit for Dissecting Dopamine Circuit Function in Drosophila |
| title_full | A Genetic Toolkit for Dissecting Dopamine Circuit Function in Drosophila |
| title_fullStr | A Genetic Toolkit for Dissecting Dopamine Circuit Function in Drosophila |
| title_full_unstemmed | A Genetic Toolkit for Dissecting Dopamine Circuit Function in Drosophila |
| title_short | A Genetic Toolkit for Dissecting Dopamine Circuit Function in Drosophila |
| title_sort | genetic toolkit for dissecting dopamine circuit function in drosophila |
| url | http://www.sciencedirect.com/science/article/pii/S2211124718304297 |
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