Expression of minichromosome maintenance genes in renal cell carcinoma
Hongbin Zhong,1,* Bin Chen,1,* Henrique Neves,2 Jinchun Xing,1 Youxin Ye,1 Ying Lin,1 Guohong Zhuang,3 Shu-Dong Zhang,4 Jiyi Huang,1,5 Hang Fai Kwok2 1Xiang’an Branch, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, People’s Republic of China; 2Faculty of Heal...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2017-11-01
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| Series: | Cancer Management and Research |
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| Online Access: | https://www.dovepress.com/expression-of-minichromosome-maintenance-genes-in-renal-cell-carcinoma-peer-reviewed-article-CMAR |
| _version_ | 1819243329005551616 |
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| author | Zhong HB Chen B Neves H Xing JC Ye YX Lin Y Zhuang GH Zhang SD Huang JY Kwok HF |
| author_facet | Zhong HB Chen B Neves H Xing JC Ye YX Lin Y Zhuang GH Zhang SD Huang JY Kwok HF |
| author_sort | Zhong HB |
| collection | DOAJ |
| description | Hongbin Zhong,1,* Bin Chen,1,* Henrique Neves,2 Jinchun Xing,1 Youxin Ye,1 Ying Lin,1 Guohong Zhuang,3 Shu-Dong Zhang,4 Jiyi Huang,1,5 Hang Fai Kwok2 1Xiang’an Branch, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, People’s Republic of China; 2Faculty of Health Sciences, University of Macau, Taipa, Macau SAR; 3Medical College of Xiamen University, Xiamen, Fujian, People’s Republic of China; 4Northern Ireland Centre for Stratified Medicine, Biomedical Sciences Research Institute, Ulster University, Londonderry, UK; 5The First Clinical School of Fujian Medical University, Fuzhou, Fujian, People’s Republic of China *These authors contributed equally to this work Abstract: Minichromosome maintenance (MCM) proteins play an essential role in DNA replication. They have been shown to be overexpressed in various types of cancer. However, the role of this family in renal cell carcinoma (RCC) is widely unknown. In this study, we have identified a number of RCC datasets in the Gene Expression Omnibus database and also investigated the correlation between the expression levels of MCM genes and clinicopathological parameters. We found that the expression levels of MCM genes are positively correlated with one another. Expression levels of MCM2, MCM5, MCM6, and MCM7, but not of MCM3 and MCM4, were higher in RCC compared to paired adjacent normal tissue. Only the expression level of MCM4, but not of other MCMs, was positively correlated with tumor grade. In addition, a high-level expression of MCM2 in either primary tumor or metastases of RCC predicted a shorter disease-free survival time, while a high-level expression of MCM4 or MCM6 in primary tumor was also associated with poorer disease-free survival. Interestingly, we also demonstrated that patients with their primary RCC overexpressing 2 or more MCM genes had a shorter disease-free survival time, while those with RCC metastases overexpressing 3 or more MCM genes had a shorter disease-free survival. Importantly, we also demonstrated that overexpression of MCM genes is an independent predictor for survival in RCC patients. Our results suggest that MCM2–7 genes may be an important prognostic marker for patients with RCC. Keywords: MCM proteins, kidney cancer, prognostic marker, metastases, survival |
| first_indexed | 2024-12-23T14:53:58Z |
| format | Article |
| id | doaj.art-3624fdb737df43aba7670dd21e0cfe0c |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-23T14:53:58Z |
| publishDate | 2017-11-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-3624fdb737df43aba7670dd21e0cfe0c2022-12-21T17:42:49ZengDove Medical PressCancer Management and Research1179-13222017-11-01Volume 963764735614Expression of minichromosome maintenance genes in renal cell carcinomaZhong HBChen BNeves HXing JCYe YXLin YZhuang GHZhang SDHuang JYKwok HFHongbin Zhong,1,* Bin Chen,1,* Henrique Neves,2 Jinchun Xing,1 Youxin Ye,1 Ying Lin,1 Guohong Zhuang,3 Shu-Dong Zhang,4 Jiyi Huang,1,5 Hang Fai Kwok2 1Xiang’an Branch, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, People’s Republic of China; 2Faculty of Health Sciences, University of Macau, Taipa, Macau SAR; 3Medical College of Xiamen University, Xiamen, Fujian, People’s Republic of China; 4Northern Ireland Centre for Stratified Medicine, Biomedical Sciences Research Institute, Ulster University, Londonderry, UK; 5The First Clinical School of Fujian Medical University, Fuzhou, Fujian, People’s Republic of China *These authors contributed equally to this work Abstract: Minichromosome maintenance (MCM) proteins play an essential role in DNA replication. They have been shown to be overexpressed in various types of cancer. However, the role of this family in renal cell carcinoma (RCC) is widely unknown. In this study, we have identified a number of RCC datasets in the Gene Expression Omnibus database and also investigated the correlation between the expression levels of MCM genes and clinicopathological parameters. We found that the expression levels of MCM genes are positively correlated with one another. Expression levels of MCM2, MCM5, MCM6, and MCM7, but not of MCM3 and MCM4, were higher in RCC compared to paired adjacent normal tissue. Only the expression level of MCM4, but not of other MCMs, was positively correlated with tumor grade. In addition, a high-level expression of MCM2 in either primary tumor or metastases of RCC predicted a shorter disease-free survival time, while a high-level expression of MCM4 or MCM6 in primary tumor was also associated with poorer disease-free survival. Interestingly, we also demonstrated that patients with their primary RCC overexpressing 2 or more MCM genes had a shorter disease-free survival time, while those with RCC metastases overexpressing 3 or more MCM genes had a shorter disease-free survival. Importantly, we also demonstrated that overexpression of MCM genes is an independent predictor for survival in RCC patients. Our results suggest that MCM2–7 genes may be an important prognostic marker for patients with RCC. Keywords: MCM proteins, kidney cancer, prognostic marker, metastases, survivalhttps://www.dovepress.com/expression-of-minichromosome-maintenance-genes-in-renal-cell-carcinoma-peer-reviewed-article-CMARMinichromosome maintenance proteinsRenal cell carcinomaPrognostic markerMetastasesSurvival |
| spellingShingle | Zhong HB Chen B Neves H Xing JC Ye YX Lin Y Zhuang GH Zhang SD Huang JY Kwok HF Expression of minichromosome maintenance genes in renal cell carcinoma Cancer Management and Research Minichromosome maintenance proteins Renal cell carcinoma Prognostic marker Metastases Survival |
| title | Expression of minichromosome maintenance genes in renal cell carcinoma |
| title_full | Expression of minichromosome maintenance genes in renal cell carcinoma |
| title_fullStr | Expression of minichromosome maintenance genes in renal cell carcinoma |
| title_full_unstemmed | Expression of minichromosome maintenance genes in renal cell carcinoma |
| title_short | Expression of minichromosome maintenance genes in renal cell carcinoma |
| title_sort | expression of minichromosome maintenance genes in renal cell carcinoma |
| topic | Minichromosome maintenance proteins Renal cell carcinoma Prognostic marker Metastases Survival |
| url | https://www.dovepress.com/expression-of-minichromosome-maintenance-genes-in-renal-cell-carcinoma-peer-reviewed-article-CMAR |
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