Regeneration of industrial sugarcane using in-vitro plant apical meristem

Regeneration of industrial sugarcane using in-vitro plant apical meristem

A rapid micro-propagation of sugarcane, Saccharum officinarum variety NCS 008 using apical meristem shoots were cultured on MS medium supplement with different concentration of BAP (0.1-0.4mg/l) and NAA (0.01-0.04mg/l) for shoot proliferation and elongation. The study showed that highest number of r...

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Bibliographic Details
Main Authors: A. Ajadi, B.O. Ehirim, M. N. Ishaq, A. Isah, A. Isong, A. Ghali, O. Kester and, A. N. Ejizu
Format: Article
Language:English
Published: Indian Society of Plant Breeders 2018-12-01
Series:Electronic Journal of Plant Breeding
Subjects:
bap
culture
iba
meristem shoots
ms
naa
sugarcane
proliferation
Online Access:http://ejplantbreeding.org/index.php/EJPB/article/view/2939
Description
Summary:A rapid micro-propagation of sugarcane, Saccharum officinarum variety NCS 008 using apical meristem shoots were cultured on MS medium supplement with different concentration of BAP (0.1-0.4mg/l) and NAA (0.01-0.04mg/l) for shoot proliferation and elongation. The study showed that highest number of regenerated shoots were obtained from MS medium supplemented with BAP (0.3mg/l) and NAA (0.03mg/l)giving an average of 8 shoot per meristem shoot cultured. Roots were induced using MS medium supplemented with different combinations of NAA and IBA. NAA (3.0mg/l) gave highest rooting percentage of 94.74. The meristem shoot tip demonstrated good response to plant regeneration of sugarcane variety NCS 008 through in-vitro culture.
ISSN:0975-928X

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