Regeneration of industrial sugarcane using in-vitro plant apical meristem
A rapid micro-propagation of sugarcane, Saccharum officinarum variety NCS 008 using apical meristem shoots were cultured on MS medium supplement with different concentration of BAP (0.1-0.4mg/l) and NAA (0.01-0.04mg/l) for shoot proliferation and elongation. The study showed that highest number of r...
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| Format: | Article |
| Language: | English |
| Published: |
Indian Society of Plant Breeders
2018-12-01
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| Series: | Electronic Journal of Plant Breeding |
| Subjects: | |
| Online Access: | http://ejplantbreeding.org/index.php/EJPB/article/view/2939 |
| _version_ | 1818409095703560192 |
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| author | A. Ajadi B.O. Ehirim M. N. Ishaq A. Isah A. Isong A. Ghali O. Kester and A. N. Ejizu |
| author_facet | A. Ajadi B.O. Ehirim M. N. Ishaq A. Isah A. Isong A. Ghali O. Kester and A. N. Ejizu |
| author_sort | A. Ajadi |
| collection | DOAJ |
| description | A rapid micro-propagation of sugarcane, Saccharum officinarum variety NCS 008 using apical meristem shoots were cultured on MS medium supplement with different concentration of BAP (0.1-0.4mg/l) and NAA (0.01-0.04mg/l) for shoot proliferation and elongation. The study showed that highest number of regenerated shoots were obtained from MS medium supplemented with BAP (0.3mg/l) and NAA (0.03mg/l)giving an average of 8 shoot per meristem shoot cultured. Roots were induced using MS medium supplemented with different combinations of NAA and IBA. NAA (3.0mg/l) gave highest rooting percentage of 94.74. The meristem shoot tip demonstrated good response to plant regeneration of sugarcane variety NCS 008 through in-vitro culture. |
| first_indexed | 2024-12-14T09:54:11Z |
| format | Article |
| id | doaj.art-3625c1cad71446aab01b902e350dce54 |
| institution | Directory Open Access Journal |
| issn | 0975-928X |
| language | English |
| last_indexed | 2024-12-14T09:54:11Z |
| publishDate | 2018-12-01 |
| publisher | Indian Society of Plant Breeders |
| record_format | Article |
| series | Electronic Journal of Plant Breeding |
| spelling | doaj.art-3625c1cad71446aab01b902e350dce542022-12-21T23:07:27ZengIndian Society of Plant BreedersElectronic Journal of Plant Breeding0975-928X2018-12-01941342134710.5958/0975-928X.2018.00167.9Regeneration of industrial sugarcane using in-vitro plant apical meristemA. AjadiB.O. EhirimM. N. IshaqA. IsahA. IsongA. GhaliO. Kester andA. N. EjizuA rapid micro-propagation of sugarcane, Saccharum officinarum variety NCS 008 using apical meristem shoots were cultured on MS medium supplement with different concentration of BAP (0.1-0.4mg/l) and NAA (0.01-0.04mg/l) for shoot proliferation and elongation. The study showed that highest number of regenerated shoots were obtained from MS medium supplemented with BAP (0.3mg/l) and NAA (0.03mg/l)giving an average of 8 shoot per meristem shoot cultured. Roots were induced using MS medium supplemented with different combinations of NAA and IBA. NAA (3.0mg/l) gave highest rooting percentage of 94.74. The meristem shoot tip demonstrated good response to plant regeneration of sugarcane variety NCS 008 through in-vitro culture.http://ejplantbreeding.org/index.php/EJPB/article/view/2939bapcultureibameristem shootsmsnaasugarcaneproliferation |
| spellingShingle | A. Ajadi B.O. Ehirim M. N. Ishaq A. Isah A. Isong A. Ghali O. Kester and A. N. Ejizu Regeneration of industrial sugarcane using in-vitro plant apical meristem Electronic Journal of Plant Breeding bap culture iba meristem shoots ms naa sugarcane proliferation |
| title | Regeneration of industrial sugarcane using in-vitro plant apical meristem |
| title_full | Regeneration of industrial sugarcane using in-vitro plant apical meristem |
| title_fullStr | Regeneration of industrial sugarcane using in-vitro plant apical meristem |
| title_full_unstemmed | Regeneration of industrial sugarcane using in-vitro plant apical meristem |
| title_short | Regeneration of industrial sugarcane using in-vitro plant apical meristem |
| title_sort | regeneration of industrial sugarcane using in vitro plant apical meristem |
| topic | bap culture iba meristem shoots ms naa sugarcane proliferation |
| url | http://ejplantbreeding.org/index.php/EJPB/article/view/2939 |
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