2,3-Butanediol and Acetoin Production from Enzymatic Hydrolysate of Ionic Liquid-pretreated Cellulose by Paenibacillus polymyxa
A safe microorganism (class 1), Paenibacillus polymyxa, was used for 2,3-butanediol and acetoin production, which could make the fermentation process cheaper and less complex. It showed a broad substrate spectrum, such as mannose, galactose, cellobiose, glycerol, the mixture of glucose and xylose, a...
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| Format: | Article |
| Language: | English |
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North Carolina State University
2015-01-01
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| Series: | BioResources |
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| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_1_1318_Jiang_Cellulose_Paenibacillus_ |
| _version_ | 1819151279154266112 |
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| author | Li-qun Jiang Zhen Fang Zeng-li Zhao Fang He Hai-bin Li |
| author_facet | Li-qun Jiang Zhen Fang Zeng-li Zhao Fang He Hai-bin Li |
| author_sort | Li-qun Jiang |
| collection | DOAJ |
| description | A safe microorganism (class 1), Paenibacillus polymyxa, was used for 2,3-butanediol and acetoin production, which could make the fermentation process cheaper and less complex. It showed a broad substrate spectrum, such as mannose, galactose, cellobiose, glycerol, the mixture of glucose and xylose, and the mixture of glucose and cellobiose. In addition, the strain can utilize highly concentrated glucose that was obtained by enzymatic hydrolysis of ionic liquid-pretreated cellulose. With a 15% initial cellulose consistency, the final glucose concentration was 109.5 g/L with 65.7% glucose yield. Without any treatment, the hydrolysate was successfully used to produce 2,3-butanediol and acetoin with a yield of 81.7% and a productivity of 0.7 g/(L•h) by Paenibacillus polymyxa. Higher concentration and higher productivity with relatively high yield, compared with previous works by acid hydrolysis, of 2,3-butanediol and acetoin were achieved. All these novel improvements offer significant opportunities to further decrease the cost of large-scale 2,3-butanediol and acetoin production. |
| first_indexed | 2024-12-22T14:30:52Z |
| format | Article |
| id | doaj.art-362787330a8d4e87bf344014c4d0f2ca |
| institution | Directory Open Access Journal |
| issn | 1930-2126 1930-2126 |
| language | English |
| last_indexed | 2024-12-22T14:30:52Z |
| publishDate | 2015-01-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-362787330a8d4e87bf344014c4d0f2ca2022-12-21T18:22:44ZengNorth Carolina State UniversityBioResources1930-21261930-21262015-01-011011318132910.15376/biores.10.1.1318-13292,3-Butanediol and Acetoin Production from Enzymatic Hydrolysate of Ionic Liquid-pretreated Cellulose by Paenibacillus polymyxaLi-qun Jiang0Zhen Fang1Zeng-li Zhao2Fang He3Hai-bin Li4Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640,China; ChinaBiomass Group, Key Laboratory of Tropical Plant Resources and Sustainable Use, Chinese Academy of Sciences, Xishuangbanna Tropical Botanical Garden, 88 Xuefulu, Kunming, Yunnan 650223, China; ChinaKey Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640,China; ChinaKey Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640,China; ChinaKey Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640,China; ChinaA safe microorganism (class 1), Paenibacillus polymyxa, was used for 2,3-butanediol and acetoin production, which could make the fermentation process cheaper and less complex. It showed a broad substrate spectrum, such as mannose, galactose, cellobiose, glycerol, the mixture of glucose and xylose, and the mixture of glucose and cellobiose. In addition, the strain can utilize highly concentrated glucose that was obtained by enzymatic hydrolysis of ionic liquid-pretreated cellulose. With a 15% initial cellulose consistency, the final glucose concentration was 109.5 g/L with 65.7% glucose yield. Without any treatment, the hydrolysate was successfully used to produce 2,3-butanediol and acetoin with a yield of 81.7% and a productivity of 0.7 g/(L•h) by Paenibacillus polymyxa. Higher concentration and higher productivity with relatively high yield, compared with previous works by acid hydrolysis, of 2,3-butanediol and acetoin were achieved. All these novel improvements offer significant opportunities to further decrease the cost of large-scale 2,3-butanediol and acetoin production.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_1_1318_Jiang_Cellulose_Paenibacillus_Paenibacillus polymyxaIonic liquid23-Butanediol2.3-ButanediolFermentationEnzymatic hydrolysis |
| spellingShingle | Li-qun Jiang Zhen Fang Zeng-li Zhao Fang He Hai-bin Li 2,3-Butanediol and Acetoin Production from Enzymatic Hydrolysate of Ionic Liquid-pretreated Cellulose by Paenibacillus polymyxa BioResources Paenibacillus polymyxa Ionic liquid 2 3-Butanediol 2.3-Butanediol Fermentation Enzymatic hydrolysis |
| title | 2,3-Butanediol and Acetoin Production from Enzymatic Hydrolysate of Ionic Liquid-pretreated Cellulose by Paenibacillus polymyxa |
| title_full | 2,3-Butanediol and Acetoin Production from Enzymatic Hydrolysate of Ionic Liquid-pretreated Cellulose by Paenibacillus polymyxa |
| title_fullStr | 2,3-Butanediol and Acetoin Production from Enzymatic Hydrolysate of Ionic Liquid-pretreated Cellulose by Paenibacillus polymyxa |
| title_full_unstemmed | 2,3-Butanediol and Acetoin Production from Enzymatic Hydrolysate of Ionic Liquid-pretreated Cellulose by Paenibacillus polymyxa |
| title_short | 2,3-Butanediol and Acetoin Production from Enzymatic Hydrolysate of Ionic Liquid-pretreated Cellulose by Paenibacillus polymyxa |
| title_sort | 2 3 butanediol and acetoin production from enzymatic hydrolysate of ionic liquid pretreated cellulose by paenibacillus polymyxa |
| topic | Paenibacillus polymyxa Ionic liquid 2 3-Butanediol 2.3-Butanediol Fermentation Enzymatic hydrolysis |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_1_1318_Jiang_Cellulose_Paenibacillus_ |
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