Connectivity and functional characterization of the mouse contralateral projecting neurons in basolateral amygdala
Amygdala is widely known as a critical neural hub for modulating negative emotions such as fear and anxiety. Recent studies have shown that the basolateral amygdala (BLA) has reciprocal innervation, which plays an important role in regulating social interaction and associative memory. However, the c...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2022-09-01
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| Series: | Stress and Brain |
| Subjects: | |
| Online Access: | https://www.sciopen.com/article/10.26599/SAB.2022.9060017 |
| _version_ | 1811175542186049536 |
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| author | Jia-Xin Zou Guan-Qun Shang Wei-Zhu Liu Ye He Wen-Hua Zhang |
| author_facet | Jia-Xin Zou Guan-Qun Shang Wei-Zhu Liu Ye He Wen-Hua Zhang |
| author_sort | Jia-Xin Zou |
| collection | DOAJ |
| description | Amygdala is widely known as a critical neural hub for modulating negative emotions such as fear and anxiety. Recent studies have shown that the basolateral amygdala (BLA) has reciprocal innervation, which plays an important role in regulating social interaction and associative memory. However, the connectivity pattern and functional role of the contralateral projecting BLA neurons in stress-related anxiety are not fully understood. Here, using the virus tracing strategy, we first characterized the connectivity pattern of the contralateral projecting neurons in BLA. Then, we examined the effect of stress on the functional changes of those specific neuronal populations using a mouse model of inescapable footshock stress. The results showed that the fibers of the contralateral projecting BLA neurons labeled by enhanced green fluorescent protein (EGFP) have no collateral projections to the ipsilateral or contralateral hippocampus. Interestingly, they have some collateral projections to the ipsilateral medial prefrontal cortex. Behavioral results showed that footshock stress increases the anxiety-like behavior in mice; however, the excitatory synaptic transmission and intrinsic excitability of the contralateral projecting BLA neurons measured by whole-cell patch clamp recording did not change after stress exposure. In conclusion, our results indicate that contralateral projecting BLA neurons may not contribute to footshock stress-related anxiety-like behavior. |
| first_indexed | 2024-04-10T19:37:26Z |
| format | Article |
| id | doaj.art-3628393e0dbc49898de66f6172c49ebc |
| institution | Directory Open Access Journal |
| issn | 2709-1325 |
| language | English |
| last_indexed | 2024-04-10T19:37:26Z |
| publishDate | 2022-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Stress and Brain |
| spelling | doaj.art-3628393e0dbc49898de66f6172c49ebc2023-01-30T04:01:09ZengTsinghua University PressStress and Brain2709-13252022-09-0123879910.26599/SAB.2022.9060017Connectivity and functional characterization of the mouse contralateral projecting neurons in basolateral amygdalaJia-Xin Zou0Guan-Qun Shang1Wei-Zhu Liu2Ye He3Wen-Hua Zhang4Department of Biological Science, School of Life Science, Nanchang University, Nanchang 330031, ChinaQueen Mary School, Nanchang University, Nanchang 330001, ChinaDepartment of Biological Science, School of Life Science, Nanchang University, Nanchang 330031, ChinaSchool of Basic Medical Sciences, Nanchang University, Nanchang 330031, ChinaDepartment of Biological Science, School of Life Science, Nanchang University, Nanchang 330031, ChinaAmygdala is widely known as a critical neural hub for modulating negative emotions such as fear and anxiety. Recent studies have shown that the basolateral amygdala (BLA) has reciprocal innervation, which plays an important role in regulating social interaction and associative memory. However, the connectivity pattern and functional role of the contralateral projecting BLA neurons in stress-related anxiety are not fully understood. Here, using the virus tracing strategy, we first characterized the connectivity pattern of the contralateral projecting neurons in BLA. Then, we examined the effect of stress on the functional changes of those specific neuronal populations using a mouse model of inescapable footshock stress. The results showed that the fibers of the contralateral projecting BLA neurons labeled by enhanced green fluorescent protein (EGFP) have no collateral projections to the ipsilateral or contralateral hippocampus. Interestingly, they have some collateral projections to the ipsilateral medial prefrontal cortex. Behavioral results showed that footshock stress increases the anxiety-like behavior in mice; however, the excitatory synaptic transmission and intrinsic excitability of the contralateral projecting BLA neurons measured by whole-cell patch clamp recording did not change after stress exposure. In conclusion, our results indicate that contralateral projecting BLA neurons may not contribute to footshock stress-related anxiety-like behavior.https://www.sciopen.com/article/10.26599/SAB.2022.9060017amygdalacontralateral connectivitystressanxietycircuit |
| spellingShingle | Jia-Xin Zou Guan-Qun Shang Wei-Zhu Liu Ye He Wen-Hua Zhang Connectivity and functional characterization of the mouse contralateral projecting neurons in basolateral amygdala Stress and Brain amygdala contralateral connectivity stress anxiety circuit |
| title | Connectivity and functional characterization of the mouse contralateral projecting neurons in basolateral amygdala |
| title_full | Connectivity and functional characterization of the mouse contralateral projecting neurons in basolateral amygdala |
| title_fullStr | Connectivity and functional characterization of the mouse contralateral projecting neurons in basolateral amygdala |
| title_full_unstemmed | Connectivity and functional characterization of the mouse contralateral projecting neurons in basolateral amygdala |
| title_short | Connectivity and functional characterization of the mouse contralateral projecting neurons in basolateral amygdala |
| title_sort | connectivity and functional characterization of the mouse contralateral projecting neurons in basolateral amygdala |
| topic | amygdala contralateral connectivity stress anxiety circuit |
| url | https://www.sciopen.com/article/10.26599/SAB.2022.9060017 |
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