Crystal Structures of Putative Flavin Dependent Monooxygenase from <i>Alicyclobacillus Acidocaldarius</i>
Flavin dependent monooxygenases catalyze various reactions to play a key role in biological processes, such as catabolism, detoxification, and biosynthesis. Group D flavin dependent monooxygenases are enzymes with an Acyl-CoA dehydrogenase (ACAD) fold and use Flavin adenine dinucleotide (FAD) or Fla...
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| Format: | Article |
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MDPI AG
2019-10-01
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| Series: | Crystals |
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| Online Access: | https://www.mdpi.com/2073-4352/9/11/548 |
| _version_ | 1798039697918787584 |
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| author | Hyunjin Moon Sungwook Shin Jungwoo Choe |
| author_facet | Hyunjin Moon Sungwook Shin Jungwoo Choe |
| author_sort | Hyunjin Moon |
| collection | DOAJ |
| description | Flavin dependent monooxygenases catalyze various reactions to play a key role in biological processes, such as catabolism, detoxification, and biosynthesis. Group D flavin dependent monooxygenases are enzymes with an Acyl-CoA dehydrogenase (ACAD) fold and use Flavin adenine dinucleotide (FAD) or Flavin mononucleotide (FMN) as a cofactor. In this research, crystal structures of <i>Alicyclobacillus acidocaldarius</i> protein formerly annotated as an ACAD were determined in Apo and FAD bound state. Although our structure showed high structural similarity to other ACADs, close comparison of substrate binding pocket and phylogenetic analysis showed that this protein is more closely related to other bacterial group D flavin dependent monooxygenases, such as DszC (sulfoxidase) and DnmZ and Kijd3 (nitrososynthases). |
| first_indexed | 2024-04-11T21:57:20Z |
| format | Article |
| id | doaj.art-362a129a3d4f4c8ebf8c3a7936dd5c79 |
| institution | Directory Open Access Journal |
| issn | 2073-4352 |
| language | English |
| last_indexed | 2024-04-11T21:57:20Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Crystals |
| spelling | doaj.art-362a129a3d4f4c8ebf8c3a7936dd5c792022-12-22T04:01:03ZengMDPI AGCrystals2073-43522019-10-0191154810.3390/cryst9110548cryst9110548Crystal Structures of Putative Flavin Dependent Monooxygenase from <i>Alicyclobacillus Acidocaldarius</i>Hyunjin Moon0Sungwook Shin1Jungwoo Choe2Department of Life Science, University of Seoul, Seoul 02504, KoreaDepartment of Life Science, University of Seoul, Seoul 02504, KoreaDepartment of Life Science, University of Seoul, Seoul 02504, KoreaFlavin dependent monooxygenases catalyze various reactions to play a key role in biological processes, such as catabolism, detoxification, and biosynthesis. Group D flavin dependent monooxygenases are enzymes with an Acyl-CoA dehydrogenase (ACAD) fold and use Flavin adenine dinucleotide (FAD) or Flavin mononucleotide (FMN) as a cofactor. In this research, crystal structures of <i>Alicyclobacillus acidocaldarius</i> protein formerly annotated as an ACAD were determined in Apo and FAD bound state. Although our structure showed high structural similarity to other ACADs, close comparison of substrate binding pocket and phylogenetic analysis showed that this protein is more closely related to other bacterial group D flavin dependent monooxygenases, such as DszC (sulfoxidase) and DnmZ and Kijd3 (nitrososynthases).https://www.mdpi.com/2073-4352/9/11/548flavin dependent monooxygenaseacyl-coa dehydrogenasefad |
| spellingShingle | Hyunjin Moon Sungwook Shin Jungwoo Choe Crystal Structures of Putative Flavin Dependent Monooxygenase from <i>Alicyclobacillus Acidocaldarius</i> Crystals flavin dependent monooxygenase acyl-coa dehydrogenase fad |
| title | Crystal Structures of Putative Flavin Dependent Monooxygenase from <i>Alicyclobacillus Acidocaldarius</i> |
| title_full | Crystal Structures of Putative Flavin Dependent Monooxygenase from <i>Alicyclobacillus Acidocaldarius</i> |
| title_fullStr | Crystal Structures of Putative Flavin Dependent Monooxygenase from <i>Alicyclobacillus Acidocaldarius</i> |
| title_full_unstemmed | Crystal Structures of Putative Flavin Dependent Monooxygenase from <i>Alicyclobacillus Acidocaldarius</i> |
| title_short | Crystal Structures of Putative Flavin Dependent Monooxygenase from <i>Alicyclobacillus Acidocaldarius</i> |
| title_sort | crystal structures of putative flavin dependent monooxygenase from i alicyclobacillus acidocaldarius i |
| topic | flavin dependent monooxygenase acyl-coa dehydrogenase fad |
| url | https://www.mdpi.com/2073-4352/9/11/548 |
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