Biosynthesized silver nanoparticles and miR34a mimics mediated activation of death receptor in MCF-7 human breast cancer cell lines
Abstract Nano-biotechnology-based clinical applications to cure health-related issues have gained huge attention among the scientific community and hold great promise to limit cancer metastasis. In this study, green-derived silver nanoparticles were synthesized by using leaf extract of Litchi chinen...
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-10-01
|
| Series: | Cancer Nanotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12645-022-00137-8 |
| _version_ | 1798029911225532416 |
|---|---|
| author | Muhammad Javed Iqbal Umer Rashid Zeeshan Javed Zara Hamid Komal Imran Ayesha Kabeer Shahid Raza Zainab M. Almarhoon Željko Reiner Iulia-Cristina Bagiu Radu Vasile Bagiu Ioan Sarac Javad Sharifi-Rad Alibek Ydyrys Sevgi Durna Daştan Monica Butnariu William C. Cho |
| author_facet | Muhammad Javed Iqbal Umer Rashid Zeeshan Javed Zara Hamid Komal Imran Ayesha Kabeer Shahid Raza Zainab M. Almarhoon Željko Reiner Iulia-Cristina Bagiu Radu Vasile Bagiu Ioan Sarac Javad Sharifi-Rad Alibek Ydyrys Sevgi Durna Daştan Monica Butnariu William C. Cho |
| author_sort | Muhammad Javed Iqbal |
| collection | DOAJ |
| description | Abstract Nano-biotechnology-based clinical applications to cure health-related issues have gained huge attention among the scientific community and hold great promise to limit cancer metastasis. In this study, green-derived silver nanoparticles were synthesized by using leaf extract of Litchi chinensis. Characterization of biosynthesized silver nanoparticles was performed by using UV–Vis spectroscopy, FTIR, XRD, EDS, and SEM analysis. The clinical application of green-drive nanoparticles was investigated by using MCF-7 cancer cell lines. MCF-7 breast cancer cell lines were analyzed against three different treatments. (i) Silver nanoparticles (AgNPs), (ii) miR34a mimics and (iii) Co-delivery of AgNPs and miR34a mimics. Cell viability was determined by MTT assay and, extraction of mRNA and cDNA synthesis were performed after successful cellular transfection. qRT-PCR was done for expression analysis of DR4 and DR5 upon exogenous delivery of all 3 treatments. Results indicate that L. chinensis leaves have a significant amount of phenolic and flavonoid contents and also possess massive antioxidant activity. The diameter of nanoparticles was observed in the range of 41–55 nm. It was concluded that green-derived silver nanoparticles can be a potential contributing agent for cancer prevention and are reported to upregulate the expression of DR4 and DR5 by 0.8-folds and 3.7-folds, respectively. |
| first_indexed | 2024-04-11T19:31:40Z |
| format | Article |
| id | doaj.art-362b130ed061421e817e08428df261ca |
| institution | Directory Open Access Journal |
| issn | 1868-6958 1868-6966 |
| language | English |
| last_indexed | 2024-04-11T19:31:40Z |
| publishDate | 2022-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Nanotechnology |
| spelling | doaj.art-362b130ed061421e817e08428df261ca2022-12-22T04:06:58ZengBMCCancer Nanotechnology1868-69581868-69662022-10-0113111410.1186/s12645-022-00137-8Biosynthesized silver nanoparticles and miR34a mimics mediated activation of death receptor in MCF-7 human breast cancer cell linesMuhammad Javed Iqbal0Umer Rashid1Zeeshan Javed2Zara Hamid3Komal Imran4Ayesha Kabeer5Shahid Raza6Zainab M. Almarhoon7Željko Reiner8Iulia-Cristina Bagiu9Radu Vasile Bagiu10Ioan Sarac11Javad Sharifi-Rad12Alibek Ydyrys13Sevgi Durna Daştan14Monica Butnariu15William C. Cho16Department of Biochemistry and Biotechnology, University of GujratDepartment of Biochemistry and Biotechnology, University of GujratOffice of Research Innovation and Commercialization, Lahore Garrison UniversityDepartment of Biochemistry and Biotechnology, University of GujratDepartment of Biotechnology, University of SialkotDepartment of Biotechnology, University of SialkotOffice of Research Innovation and Commercialization, Lahore Garrison UniversityDepartment of Chemistry, College of Science, King Saud UniversityUniversity Hospital Centre ZagrebDepartment of Microbiology, Victor Babes University of Medicine and Pharmacy of TimisoaraPreventive Medicine Study Center, Victor Babes University of Medicine and Pharmacy of Timisoara Department of MicrobiologyUniversity of Life Sciences “King Mihai I” from Timisoara, 300645, Calea Aradului 119Facultad de Medicina, Universidad del AzuayBiomedical Research Centre, Al-Farabi Kazakh National UniversityDepartment of Biology, Faculty of Science, Sivas Cumhuriyet UniversityUniversity of Life Sciences “King Mihai I” from Timisoara, 300645, Calea Aradului 119Department of Clinical Oncology, Queen Elizabeth HospitalAbstract Nano-biotechnology-based clinical applications to cure health-related issues have gained huge attention among the scientific community and hold great promise to limit cancer metastasis. In this study, green-derived silver nanoparticles were synthesized by using leaf extract of Litchi chinensis. Characterization of biosynthesized silver nanoparticles was performed by using UV–Vis spectroscopy, FTIR, XRD, EDS, and SEM analysis. The clinical application of green-drive nanoparticles was investigated by using MCF-7 cancer cell lines. MCF-7 breast cancer cell lines were analyzed against three different treatments. (i) Silver nanoparticles (AgNPs), (ii) miR34a mimics and (iii) Co-delivery of AgNPs and miR34a mimics. Cell viability was determined by MTT assay and, extraction of mRNA and cDNA synthesis were performed after successful cellular transfection. qRT-PCR was done for expression analysis of DR4 and DR5 upon exogenous delivery of all 3 treatments. Results indicate that L. chinensis leaves have a significant amount of phenolic and flavonoid contents and also possess massive antioxidant activity. The diameter of nanoparticles was observed in the range of 41–55 nm. It was concluded that green-derived silver nanoparticles can be a potential contributing agent for cancer prevention and are reported to upregulate the expression of DR4 and DR5 by 0.8-folds and 3.7-folds, respectively.https://doi.org/10.1186/s12645-022-00137-8MCF-7 cell linesSilver nanoparticles synthesisDR4DR5Breast cancer |
| spellingShingle | Muhammad Javed Iqbal Umer Rashid Zeeshan Javed Zara Hamid Komal Imran Ayesha Kabeer Shahid Raza Zainab M. Almarhoon Željko Reiner Iulia-Cristina Bagiu Radu Vasile Bagiu Ioan Sarac Javad Sharifi-Rad Alibek Ydyrys Sevgi Durna Daştan Monica Butnariu William C. Cho Biosynthesized silver nanoparticles and miR34a mimics mediated activation of death receptor in MCF-7 human breast cancer cell lines Cancer Nanotechnology MCF-7 cell lines Silver nanoparticles synthesis DR4 DR5 Breast cancer |
| title | Biosynthesized silver nanoparticles and miR34a mimics mediated activation of death receptor in MCF-7 human breast cancer cell lines |
| title_full | Biosynthesized silver nanoparticles and miR34a mimics mediated activation of death receptor in MCF-7 human breast cancer cell lines |
| title_fullStr | Biosynthesized silver nanoparticles and miR34a mimics mediated activation of death receptor in MCF-7 human breast cancer cell lines |
| title_full_unstemmed | Biosynthesized silver nanoparticles and miR34a mimics mediated activation of death receptor in MCF-7 human breast cancer cell lines |
| title_short | Biosynthesized silver nanoparticles and miR34a mimics mediated activation of death receptor in MCF-7 human breast cancer cell lines |
| title_sort | biosynthesized silver nanoparticles and mir34a mimics mediated activation of death receptor in mcf 7 human breast cancer cell lines |
| topic | MCF-7 cell lines Silver nanoparticles synthesis DR4 DR5 Breast cancer |
| url | https://doi.org/10.1186/s12645-022-00137-8 |
| work_keys_str_mv | AT muhammadjavediqbal biosynthesizedsilvernanoparticlesandmir34amimicsmediatedactivationofdeathreceptorinmcf7humanbreastcancercelllines AT umerrashid biosynthesizedsilvernanoparticlesandmir34amimicsmediatedactivationofdeathreceptorinmcf7humanbreastcancercelllines AT zeeshanjaved biosynthesizedsilvernanoparticlesandmir34amimicsmediatedactivationofdeathreceptorinmcf7humanbreastcancercelllines AT zarahamid biosynthesizedsilvernanoparticlesandmir34amimicsmediatedactivationofdeathreceptorinmcf7humanbreastcancercelllines AT komalimran biosynthesizedsilvernanoparticlesandmir34amimicsmediatedactivationofdeathreceptorinmcf7humanbreastcancercelllines AT ayeshakabeer biosynthesizedsilvernanoparticlesandmir34amimicsmediatedactivationofdeathreceptorinmcf7humanbreastcancercelllines AT shahidraza biosynthesizedsilvernanoparticlesandmir34amimicsmediatedactivationofdeathreceptorinmcf7humanbreastcancercelllines AT zainabmalmarhoon biosynthesizedsilvernanoparticlesandmir34amimicsmediatedactivationofdeathreceptorinmcf7humanbreastcancercelllines AT zeljkoreiner biosynthesizedsilvernanoparticlesandmir34amimicsmediatedactivationofdeathreceptorinmcf7humanbreastcancercelllines AT iuliacristinabagiu biosynthesizedsilvernanoparticlesandmir34amimicsmediatedactivationofdeathreceptorinmcf7humanbreastcancercelllines AT raduvasilebagiu biosynthesizedsilvernanoparticlesandmir34amimicsmediatedactivationofdeathreceptorinmcf7humanbreastcancercelllines AT ioansarac biosynthesizedsilvernanoparticlesandmir34amimicsmediatedactivationofdeathreceptorinmcf7humanbreastcancercelllines AT javadsharifirad biosynthesizedsilvernanoparticlesandmir34amimicsmediatedactivationofdeathreceptorinmcf7humanbreastcancercelllines AT alibekydyrys biosynthesizedsilvernanoparticlesandmir34amimicsmediatedactivationofdeathreceptorinmcf7humanbreastcancercelllines AT sevgidurnadastan biosynthesizedsilvernanoparticlesandmir34amimicsmediatedactivationofdeathreceptorinmcf7humanbreastcancercelllines AT monicabutnariu biosynthesizedsilvernanoparticlesandmir34amimicsmediatedactivationofdeathreceptorinmcf7humanbreastcancercelllines AT williamccho biosynthesizedsilvernanoparticlesandmir34amimicsmediatedactivationofdeathreceptorinmcf7humanbreastcancercelllines |