Exploring fungal endophytes as biocontrol agents against rice blast disease
The rice blast disease caused by Pyricularia oryzae Cavara is among the most severe plant diseases in the world. The utilization of natural antagonists, such as fungal endophytes, can be a good method to control the spread of this disease. In the present study, 68 fungal isolates were obtained fro...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Polish Botanical Society
2024-02-01
|
| Series: | Acta Agrobotanica |
| Subjects: | |
| Online Access: | https://www.journalssystem.com/agb/Exploring-fungal-endophytes-as-biocontrol-agents-against-rice-blast-disease,182943,0,2.html |
| _version_ | 1797265995463655424 |
|---|---|
| author | Mohammad Reza Safari Motlagh Dariusz Kulus Behzad Kaviani Hadi Habibollahi |
| author_facet | Mohammad Reza Safari Motlagh Dariusz Kulus Behzad Kaviani Hadi Habibollahi |
| author_sort | Mohammad Reza Safari Motlagh |
| collection | DOAJ |
| description | The rice blast disease caused by Pyricularia oryzae Cavara is among the most severe plant diseases in the world. The utilization of natural antagonists, such as fungal endophytes, can be a good method to control the spread of this disease. In the present study, 68 fungal isolates were obtained from 160 rice samples collected in Guilan province fields, Iran. Morphological and molecular characteristics were used for the identification of these fungi. According to the results, the fungal isolates were Alternaria citri (Penz.) Mussat, Alternaria infectoria Simmons, Aspergillus fumigatus Fresen, Fusarium verticillioides (Sacc.) Nirenberg, Trichoderma harzianum Rifai, Trichoderma virens (Mill., Giddens & Foster) Arx, Trichoderma viride Pers., Ulocladium consortiale (Thum) Simmons, and Pyricularia oryzae . Eight isolates that did not cause any plant disease were selected for biocontrol studies in vitro . The dual culture, culture filtrate, slide culture, and volatile metabolites methods were used in search of natural endophytic antagonists of Pyricularia oryzae . It was found that T. harzianum, T. virens, T. viride, A. fumigatus , and A. citri isolates caused the highest percentage of mycelial growth inhibition of P. oryzae in vitro . In the greenhouse experiments, all of the studied isolates reduced the P. oryzae disease rating, with T. harzianum isolate being the most effective antagonist (27.36% reduction in the disease rating), followed by T. virens, T. viride , and A. fumigatus (20.75%, 17.92%, and 16.98%, respectively). Furthermore, in greenhouse conditions, these antagonistic fungi increased the height, fresh weight, and dry weight of plants. The results of this research showed that there are various fungi in the natural flora of rice plants that possess potential biological properties against rice blast disease that can be implemented into practice. |
| first_indexed | 2024-04-25T00:53:39Z |
| format | Article |
| id | doaj.art-362bac576966443ebcf0e12123ef4cca |
| institution | Directory Open Access Journal |
| issn | 0065-0951 2300-357X |
| language | English |
| last_indexed | 2024-04-25T00:53:39Z |
| publishDate | 2024-02-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Agrobotanica |
| spelling | doaj.art-362bac576966443ebcf0e12123ef4cca2024-03-11T12:00:37ZengPolish Botanical SocietyActa Agrobotanica0065-09512300-357X2024-02-017611310.5586/aa/182943182943Exploring fungal endophytes as biocontrol agents against rice blast diseaseMohammad Reza Safari Motlagh0https://orcid.org/0000-0002-1939-2679Dariusz Kulus1https://orcid.org/0000-0001-5826-6950Behzad Kaviani2https://orcid.org/0000-0002-3583-561XHadi Habibollahi3https://orcid.org/0000-0002-3574-3870Department of Plant Protection, Islamic Azad University, IranLaboratory of Horticulture, Faculty of Agriculture and Biotechnology, Bydgoszcz University of Science and Technology, Bernardyńska 6, 85-029, Bydgoszcz, PolandDepartment of Horticultural Science, Rasht Branch, Islamic Azad University, Pole-e-Taleshan Street, Rasht City 4147654919, IranDepartment of Biology, Rasht Branch, Islamic Azad University, Pole-e-Taleshan Street, Rasht City 4147654919, IranThe rice blast disease caused by Pyricularia oryzae Cavara is among the most severe plant diseases in the world. The utilization of natural antagonists, such as fungal endophytes, can be a good method to control the spread of this disease. In the present study, 68 fungal isolates were obtained from 160 rice samples collected in Guilan province fields, Iran. Morphological and molecular characteristics were used for the identification of these fungi. According to the results, the fungal isolates were Alternaria citri (Penz.) Mussat, Alternaria infectoria Simmons, Aspergillus fumigatus Fresen, Fusarium verticillioides (Sacc.) Nirenberg, Trichoderma harzianum Rifai, Trichoderma virens (Mill., Giddens & Foster) Arx, Trichoderma viride Pers., Ulocladium consortiale (Thum) Simmons, and Pyricularia oryzae . Eight isolates that did not cause any plant disease were selected for biocontrol studies in vitro . The dual culture, culture filtrate, slide culture, and volatile metabolites methods were used in search of natural endophytic antagonists of Pyricularia oryzae . It was found that T. harzianum, T. virens, T. viride, A. fumigatus , and A. citri isolates caused the highest percentage of mycelial growth inhibition of P. oryzae in vitro . In the greenhouse experiments, all of the studied isolates reduced the P. oryzae disease rating, with T. harzianum isolate being the most effective antagonist (27.36% reduction in the disease rating), followed by T. virens, T. viride , and A. fumigatus (20.75%, 17.92%, and 16.98%, respectively). Furthermore, in greenhouse conditions, these antagonistic fungi increased the height, fresh weight, and dry weight of plants. The results of this research showed that there are various fungi in the natural flora of rice plants that possess potential biological properties against rice blast disease that can be implemented into practice.https://www.journalssystem.com/agb/Exploring-fungal-endophytes-as-biocontrol-agents-against-rice-blast-disease,182943,0,2.htmlagricultureantagonistic fungidisease ratingfungal diseasesmycelial growth inhibitionvolatile metabolites |
| spellingShingle | Mohammad Reza Safari Motlagh Dariusz Kulus Behzad Kaviani Hadi Habibollahi Exploring fungal endophytes as biocontrol agents against rice blast disease Acta Agrobotanica agriculture antagonistic fungi disease rating fungal diseases mycelial growth inhibition volatile metabolites |
| title | Exploring fungal endophytes as biocontrol agents against rice blast disease |
| title_full | Exploring fungal endophytes as biocontrol agents against rice blast disease |
| title_fullStr | Exploring fungal endophytes as biocontrol agents against rice blast disease |
| title_full_unstemmed | Exploring fungal endophytes as biocontrol agents against rice blast disease |
| title_short | Exploring fungal endophytes as biocontrol agents against rice blast disease |
| title_sort | exploring fungal endophytes as biocontrol agents against rice blast disease |
| topic | agriculture antagonistic fungi disease rating fungal diseases mycelial growth inhibition volatile metabolites |
| url | https://www.journalssystem.com/agb/Exploring-fungal-endophytes-as-biocontrol-agents-against-rice-blast-disease,182943,0,2.html |
| work_keys_str_mv | AT mohammadrezasafarimotlagh exploringfungalendophytesasbiocontrolagentsagainstriceblastdisease AT dariuszkulus exploringfungalendophytesasbiocontrolagentsagainstriceblastdisease AT behzadkaviani exploringfungalendophytesasbiocontrolagentsagainstriceblastdisease AT hadihabibollahi exploringfungalendophytesasbiocontrolagentsagainstriceblastdisease |