Immunohistochemical Expression Patterns of Tight Junction Proteins, Pro-Apoptotic and Anti-Apoptotic Factors on Progression of Intestinal Mucositis of Onco-Hematological Patients under Epirubicin-Based Chemotherapy
Chemotherapy and radiation are often accompanied by complications such as intestinal mucositis. The aim of this study was to assess by immunohistochemical assay the consequences of epirubicin-based therapy applied to onco-hematological patients, on the mucosal cells that undergo apoptosis and on the...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Online Access: | https://www.mdpi.com/2076-3417/11/10/4710 |
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| author | Coralia Cotoraci Alciona Sasu Eftimie Miutescu Dana Iovanescu Sami Gharbia Alina Ciceu Hildegard Herman Anca Hermenean |
| author_facet | Coralia Cotoraci Alciona Sasu Eftimie Miutescu Dana Iovanescu Sami Gharbia Alina Ciceu Hildegard Herman Anca Hermenean |
| author_sort | Coralia Cotoraci |
| collection | DOAJ |
| description | Chemotherapy and radiation are often accompanied by complications such as intestinal mucositis. The aim of this study was to assess by immunohistochemical assay the consequences of epirubicin-based therapy applied to onco-hematological patients, on the mucosal cells that undergo apoptosis and on the tight junction proteins, immediately before and after a short time of chemotherapy administration. We assessed the protein expression and distribution of the pro-apoptotic Bax, anti-apoptotic Bcl-2 and effector Caspase-3 as key proteins in apoptosis pathways and the changes in immunopositivity of Claudin-1 and ZO-1 tight junction proteins. Results show that the Bcl-2 family is involved in intestinal damage via Caspase-3 dependent apoptosis of epithelial cells. Additionally, the intestinal mucositis activates other injurious pathways through a dramatic drop in Claudin-1 and ZO-1 expressions, contributing for a while to a structural and functional integrity disruption of the intestinal epithelium. |
| first_indexed | 2024-03-10T11:13:04Z |
| format | Article |
| id | doaj.art-362e76e794e847abb9e46733f9db7cb2 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T11:13:04Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-362e76e794e847abb9e46733f9db7cb22023-11-21T20:39:10ZengMDPI AGApplied Sciences2076-34172021-05-011110471010.3390/app11104710Immunohistochemical Expression Patterns of Tight Junction Proteins, Pro-Apoptotic and Anti-Apoptotic Factors on Progression of Intestinal Mucositis of Onco-Hematological Patients under Epirubicin-Based ChemotherapyCoralia Cotoraci0Alciona Sasu1Eftimie Miutescu2Dana Iovanescu3Sami Gharbia4Alina Ciceu5Hildegard Herman6Anca Hermenean7Department of Hematology, Faculty of Medicine, Vasile Goldis Western University of Arad, 310025 Arad, RomaniaDepartment of Hematology, Faculty of Medicine, Vasile Goldis Western University of Arad, 310025 Arad, RomaniaDepartment of Gastroenterology, Faculty of Medicine, Vasile Goldis Western University of Arad, 310025 Arad, RomaniaDepartment of Gastroenterology, Faculty of Medicine, Vasile Goldis Western University of Arad, 310025 Arad, Romania“Aurel Ardelean” Institute of Life Sciences, Vasile Goldis Western University of Arad, 310025 Arad, Romania“Aurel Ardelean” Institute of Life Sciences, Vasile Goldis Western University of Arad, 310025 Arad, Romania“Aurel Ardelean” Institute of Life Sciences, Vasile Goldis Western University of Arad, 310025 Arad, Romania“Aurel Ardelean” Institute of Life Sciences, Vasile Goldis Western University of Arad, 310025 Arad, RomaniaChemotherapy and radiation are often accompanied by complications such as intestinal mucositis. The aim of this study was to assess by immunohistochemical assay the consequences of epirubicin-based therapy applied to onco-hematological patients, on the mucosal cells that undergo apoptosis and on the tight junction proteins, immediately before and after a short time of chemotherapy administration. We assessed the protein expression and distribution of the pro-apoptotic Bax, anti-apoptotic Bcl-2 and effector Caspase-3 as key proteins in apoptosis pathways and the changes in immunopositivity of Claudin-1 and ZO-1 tight junction proteins. Results show that the Bcl-2 family is involved in intestinal damage via Caspase-3 dependent apoptosis of epithelial cells. Additionally, the intestinal mucositis activates other injurious pathways through a dramatic drop in Claudin-1 and ZO-1 expressions, contributing for a while to a structural and functional integrity disruption of the intestinal epithelium.https://www.mdpi.com/2076-3417/11/10/4710onco-hematological patientsepirubicincolonapoptosistight junction |
| spellingShingle | Coralia Cotoraci Alciona Sasu Eftimie Miutescu Dana Iovanescu Sami Gharbia Alina Ciceu Hildegard Herman Anca Hermenean Immunohistochemical Expression Patterns of Tight Junction Proteins, Pro-Apoptotic and Anti-Apoptotic Factors on Progression of Intestinal Mucositis of Onco-Hematological Patients under Epirubicin-Based Chemotherapy Applied Sciences onco-hematological patients epirubicin colon apoptosis tight junction |
| title | Immunohistochemical Expression Patterns of Tight Junction Proteins, Pro-Apoptotic and Anti-Apoptotic Factors on Progression of Intestinal Mucositis of Onco-Hematological Patients under Epirubicin-Based Chemotherapy |
| title_full | Immunohistochemical Expression Patterns of Tight Junction Proteins, Pro-Apoptotic and Anti-Apoptotic Factors on Progression of Intestinal Mucositis of Onco-Hematological Patients under Epirubicin-Based Chemotherapy |
| title_fullStr | Immunohistochemical Expression Patterns of Tight Junction Proteins, Pro-Apoptotic and Anti-Apoptotic Factors on Progression of Intestinal Mucositis of Onco-Hematological Patients under Epirubicin-Based Chemotherapy |
| title_full_unstemmed | Immunohistochemical Expression Patterns of Tight Junction Proteins, Pro-Apoptotic and Anti-Apoptotic Factors on Progression of Intestinal Mucositis of Onco-Hematological Patients under Epirubicin-Based Chemotherapy |
| title_short | Immunohistochemical Expression Patterns of Tight Junction Proteins, Pro-Apoptotic and Anti-Apoptotic Factors on Progression of Intestinal Mucositis of Onco-Hematological Patients under Epirubicin-Based Chemotherapy |
| title_sort | immunohistochemical expression patterns of tight junction proteins pro apoptotic and anti apoptotic factors on progression of intestinal mucositis of onco hematological patients under epirubicin based chemotherapy |
| topic | onco-hematological patients epirubicin colon apoptosis tight junction |
| url | https://www.mdpi.com/2076-3417/11/10/4710 |
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