The Detection of SARS-CoV-2 Antibodies in an Exposed Human Population Is Biased by the Immunoassay Used: Implications in Serosurveillance
The presence of SARS-CoV-2 antibodies was examined over 7 months in a population of essential service workers exposed during the first epidemic wave in Madrid (Spain). Results obtained with different serological assays were compared. Firstly, serum samples obtained in April 2020 were analyzed using...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/12/11/1360 |
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| author | Francisco Llorente Elisa Pérez-Ramírez Mayte Pérez-Olmeda Desirée Dafouz-Bustos Jovita Fernández-Pinero Mercedes Martínez-Cortés Miguel Ángel Jiménez-Clavero |
| author_facet | Francisco Llorente Elisa Pérez-Ramírez Mayte Pérez-Olmeda Desirée Dafouz-Bustos Jovita Fernández-Pinero Mercedes Martínez-Cortés Miguel Ángel Jiménez-Clavero |
| author_sort | Francisco Llorente |
| collection | DOAJ |
| description | The presence of SARS-CoV-2 antibodies was examined over 7 months in a population of essential service workers exposed during the first epidemic wave in Madrid (Spain). Results obtained with different serological assays were compared. Firstly, serum samples obtained in April 2020 were analyzed using eleven SARS-CoV-2 antibody detection methods, including seven ELISAs, two CLIAs and two LFAs. While all of the ELISA tests and the Roche eCLIA method showed good performance, it was poorer for the Abbott CLIA and LFA tests. Sera from 115 workers with serologically positive results in April were collected 2 and 7 months after the first sampling and were analyzed using five of the tests previously assessed. The results showed that while some ELISA tests consistently detected the presence of anti-SARS-CoV-2 antibodies even 7 months after first detection, other methods, such as the Abbott CLIA test, showed an important reduction in sensitivity for these mature antibodies. The sensitivity increased after establishing new cut-off values, calculated taking into account both recent and old infections, suggesting that an adjustment of assay parameters may improve the detection of individuals exposed to the infection. |
| first_indexed | 2024-03-09T16:32:07Z |
| format | Article |
| id | doaj.art-3630884fcc41449c9072bbc00484be62 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T16:32:07Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-3630884fcc41449c9072bbc00484be622023-11-24T14:59:54ZengMDPI AGPathogens2076-08172023-11-011211136010.3390/pathogens12111360The Detection of SARS-CoV-2 Antibodies in an Exposed Human Population Is Biased by the Immunoassay Used: Implications in SerosurveillanceFrancisco Llorente0Elisa Pérez-Ramírez1Mayte Pérez-Olmeda2Desirée Dafouz-Bustos3Jovita Fernández-Pinero4Mercedes Martínez-Cortés5Miguel Ángel Jiménez-Clavero6Centro de Investigación en Sanidad Animal (CISA-INIA), CSIC, Valdeolmos, 28130 Madrid, SpainCentro de Investigación en Sanidad Animal (CISA-INIA), CSIC, Valdeolmos, 28130 Madrid, SpainCentro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, SpainCentro de Investigación en Sanidad Animal (CISA-INIA), CSIC, Valdeolmos, 28130 Madrid, SpainCentro de Investigación en Sanidad Animal (CISA-INIA), CSIC, Valdeolmos, 28130 Madrid, SpainMadrid Salud, Madrid City Council, 28007 Madrid, SpainCentro de Investigación en Sanidad Animal (CISA-INIA), CSIC, Valdeolmos, 28130 Madrid, SpainThe presence of SARS-CoV-2 antibodies was examined over 7 months in a population of essential service workers exposed during the first epidemic wave in Madrid (Spain). Results obtained with different serological assays were compared. Firstly, serum samples obtained in April 2020 were analyzed using eleven SARS-CoV-2 antibody detection methods, including seven ELISAs, two CLIAs and two LFAs. While all of the ELISA tests and the Roche eCLIA method showed good performance, it was poorer for the Abbott CLIA and LFA tests. Sera from 115 workers with serologically positive results in April were collected 2 and 7 months after the first sampling and were analyzed using five of the tests previously assessed. The results showed that while some ELISA tests consistently detected the presence of anti-SARS-CoV-2 antibodies even 7 months after first detection, other methods, such as the Abbott CLIA test, showed an important reduction in sensitivity for these mature antibodies. The sensitivity increased after establishing new cut-off values, calculated taking into account both recent and old infections, suggesting that an adjustment of assay parameters may improve the detection of individuals exposed to the infection.https://www.mdpi.com/2076-0817/12/11/1360COVID-19SARS-CoV-2serologic assaysELISACLIALFA |
| spellingShingle | Francisco Llorente Elisa Pérez-Ramírez Mayte Pérez-Olmeda Desirée Dafouz-Bustos Jovita Fernández-Pinero Mercedes Martínez-Cortés Miguel Ángel Jiménez-Clavero The Detection of SARS-CoV-2 Antibodies in an Exposed Human Population Is Biased by the Immunoassay Used: Implications in Serosurveillance Pathogens COVID-19 SARS-CoV-2 serologic assays ELISA CLIA LFA |
| title | The Detection of SARS-CoV-2 Antibodies in an Exposed Human Population Is Biased by the Immunoassay Used: Implications in Serosurveillance |
| title_full | The Detection of SARS-CoV-2 Antibodies in an Exposed Human Population Is Biased by the Immunoassay Used: Implications in Serosurveillance |
| title_fullStr | The Detection of SARS-CoV-2 Antibodies in an Exposed Human Population Is Biased by the Immunoassay Used: Implications in Serosurveillance |
| title_full_unstemmed | The Detection of SARS-CoV-2 Antibodies in an Exposed Human Population Is Biased by the Immunoassay Used: Implications in Serosurveillance |
| title_short | The Detection of SARS-CoV-2 Antibodies in an Exposed Human Population Is Biased by the Immunoassay Used: Implications in Serosurveillance |
| title_sort | detection of sars cov 2 antibodies in an exposed human population is biased by the immunoassay used implications in serosurveillance |
| topic | COVID-19 SARS-CoV-2 serologic assays ELISA CLIA LFA |
| url | https://www.mdpi.com/2076-0817/12/11/1360 |
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