A Mutation in <i>CsYL2.1</i> Encoding a Plastid Isoform of Triose Phosphate Isomerase Leads to <i>Yellow Leaf 2.1</i> (<i>yl2.1</i>) in Cucumber (<i>Cucumis Sativus</i> L.)
The leaf is an important photosynthetic organ and plays an essential role in the growth and development of plants. Leaf color mutants are ideal materials for studying chlorophyll metabolism, chloroplast development, and photosynthesis. In this study, we identified an EMS-induced mutant, <i>yl2...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-12-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/1/322 |
| _version_ | 1797543004957835264 |
|---|---|
| author | Liangrong Xiong Hui Du Keyan Zhang Duo Lv Huanle He Junsong Pan Run Cai Gang Wang |
| author_facet | Liangrong Xiong Hui Du Keyan Zhang Duo Lv Huanle He Junsong Pan Run Cai Gang Wang |
| author_sort | Liangrong Xiong |
| collection | DOAJ |
| description | The leaf is an important photosynthetic organ and plays an essential role in the growth and development of plants. Leaf color mutants are ideal materials for studying chlorophyll metabolism, chloroplast development, and photosynthesis. In this study, we identified an EMS-induced mutant, <i>yl2.1</i>, which exhibited yellow cotyledons and true leaves that did not turn green with leaf growth. The <i>yl2.1</i> locus was controlled by a recessive nuclear gene. The <i>CsYL2.1</i> was mapped to a 166.7-kb genomic region on chromosome 2, which contains 24 predicted genes. Only one non-synonymous single nucleotide polymorphism (SNP) was found between <i>yl2.1</i> and wt-WD1 that was located in Exon 7 of <i>Csa2G263900</i>, resulting in an amino acid substitution. <i>CsYL2.1</i> encodes a plastid isoform of triose phosphate isomerase (pdTPI), which catalyzes the reversible conversion of dihydroxyacetone phosphate (DHAP) to glyceraldehyde-3-phosphate (GAP) in chloroplasts. <i>CsYL2.1</i> was highly expressed in the cotyledons and leaves. The mesophyll cells of the <i>yl2.1</i> leaves contained reduced chlorophyll and abnormal chloroplasts. Correspondingly, the photosynthetic efficiency of the <i>yl2.1</i> leaves was impaired. Identification of <i>CsYL2.1</i> is helpful in elucidating the function of ptTPI in the chlorophyll metabolism and chloroplast development and understanding the molecular mechanism of this leaf color variant in cucumber. |
| first_indexed | 2024-03-10T13:39:30Z |
| format | Article |
| id | doaj.art-3637f7efa2b74dc8b240e7c07b2760a4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T13:39:30Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3637f7efa2b74dc8b240e7c07b2760a42023-11-21T03:10:28ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-12-0122132210.3390/ijms22010322A Mutation in <i>CsYL2.1</i> Encoding a Plastid Isoform of Triose Phosphate Isomerase Leads to <i>Yellow Leaf 2.1</i> (<i>yl2.1</i>) in Cucumber (<i>Cucumis Sativus</i> L.)Liangrong Xiong0Hui Du1Keyan Zhang2Duo Lv3Huanle He4Junsong Pan5Run Cai6Gang Wang7School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, ChinaThe leaf is an important photosynthetic organ and plays an essential role in the growth and development of plants. Leaf color mutants are ideal materials for studying chlorophyll metabolism, chloroplast development, and photosynthesis. In this study, we identified an EMS-induced mutant, <i>yl2.1</i>, which exhibited yellow cotyledons and true leaves that did not turn green with leaf growth. The <i>yl2.1</i> locus was controlled by a recessive nuclear gene. The <i>CsYL2.1</i> was mapped to a 166.7-kb genomic region on chromosome 2, which contains 24 predicted genes. Only one non-synonymous single nucleotide polymorphism (SNP) was found between <i>yl2.1</i> and wt-WD1 that was located in Exon 7 of <i>Csa2G263900</i>, resulting in an amino acid substitution. <i>CsYL2.1</i> encodes a plastid isoform of triose phosphate isomerase (pdTPI), which catalyzes the reversible conversion of dihydroxyacetone phosphate (DHAP) to glyceraldehyde-3-phosphate (GAP) in chloroplasts. <i>CsYL2.1</i> was highly expressed in the cotyledons and leaves. The mesophyll cells of the <i>yl2.1</i> leaves contained reduced chlorophyll and abnormal chloroplasts. Correspondingly, the photosynthetic efficiency of the <i>yl2.1</i> leaves was impaired. Identification of <i>CsYL2.1</i> is helpful in elucidating the function of ptTPI in the chlorophyll metabolism and chloroplast development and understanding the molecular mechanism of this leaf color variant in cucumber.https://www.mdpi.com/1422-0067/22/1/322cucumberyellow leafchlorophyll metabolismchloroplast developmentptTPI |
| spellingShingle | Liangrong Xiong Hui Du Keyan Zhang Duo Lv Huanle He Junsong Pan Run Cai Gang Wang A Mutation in <i>CsYL2.1</i> Encoding a Plastid Isoform of Triose Phosphate Isomerase Leads to <i>Yellow Leaf 2.1</i> (<i>yl2.1</i>) in Cucumber (<i>Cucumis Sativus</i> L.) International Journal of Molecular Sciences cucumber yellow leaf chlorophyll metabolism chloroplast development ptTPI |
| title | A Mutation in <i>CsYL2.1</i> Encoding a Plastid Isoform of Triose Phosphate Isomerase Leads to <i>Yellow Leaf 2.1</i> (<i>yl2.1</i>) in Cucumber (<i>Cucumis Sativus</i> L.) |
| title_full | A Mutation in <i>CsYL2.1</i> Encoding a Plastid Isoform of Triose Phosphate Isomerase Leads to <i>Yellow Leaf 2.1</i> (<i>yl2.1</i>) in Cucumber (<i>Cucumis Sativus</i> L.) |
| title_fullStr | A Mutation in <i>CsYL2.1</i> Encoding a Plastid Isoform of Triose Phosphate Isomerase Leads to <i>Yellow Leaf 2.1</i> (<i>yl2.1</i>) in Cucumber (<i>Cucumis Sativus</i> L.) |
| title_full_unstemmed | A Mutation in <i>CsYL2.1</i> Encoding a Plastid Isoform of Triose Phosphate Isomerase Leads to <i>Yellow Leaf 2.1</i> (<i>yl2.1</i>) in Cucumber (<i>Cucumis Sativus</i> L.) |
| title_short | A Mutation in <i>CsYL2.1</i> Encoding a Plastid Isoform of Triose Phosphate Isomerase Leads to <i>Yellow Leaf 2.1</i> (<i>yl2.1</i>) in Cucumber (<i>Cucumis Sativus</i> L.) |
| title_sort | mutation in i csyl2 1 i encoding a plastid isoform of triose phosphate isomerase leads to i yellow leaf 2 1 i i yl2 1 i in cucumber i cucumis sativus i l |
| topic | cucumber yellow leaf chlorophyll metabolism chloroplast development ptTPI |
| url | https://www.mdpi.com/1422-0067/22/1/322 |
| work_keys_str_mv | AT liangrongxiong amutationinicsyl21iencodingaplastidisoformoftriosephosphateisomeraseleadstoiyellowleaf21iiyl21iincucumbericucumissativusil AT huidu amutationinicsyl21iencodingaplastidisoformoftriosephosphateisomeraseleadstoiyellowleaf21iiyl21iincucumbericucumissativusil AT keyanzhang amutationinicsyl21iencodingaplastidisoformoftriosephosphateisomeraseleadstoiyellowleaf21iiyl21iincucumbericucumissativusil AT duolv amutationinicsyl21iencodingaplastidisoformoftriosephosphateisomeraseleadstoiyellowleaf21iiyl21iincucumbericucumissativusil AT huanlehe amutationinicsyl21iencodingaplastidisoformoftriosephosphateisomeraseleadstoiyellowleaf21iiyl21iincucumbericucumissativusil AT junsongpan amutationinicsyl21iencodingaplastidisoformoftriosephosphateisomeraseleadstoiyellowleaf21iiyl21iincucumbericucumissativusil AT runcai amutationinicsyl21iencodingaplastidisoformoftriosephosphateisomeraseleadstoiyellowleaf21iiyl21iincucumbericucumissativusil AT gangwang amutationinicsyl21iencodingaplastidisoformoftriosephosphateisomeraseleadstoiyellowleaf21iiyl21iincucumbericucumissativusil AT liangrongxiong mutationinicsyl21iencodingaplastidisoformoftriosephosphateisomeraseleadstoiyellowleaf21iiyl21iincucumbericucumissativusil AT huidu mutationinicsyl21iencodingaplastidisoformoftriosephosphateisomeraseleadstoiyellowleaf21iiyl21iincucumbericucumissativusil AT keyanzhang mutationinicsyl21iencodingaplastidisoformoftriosephosphateisomeraseleadstoiyellowleaf21iiyl21iincucumbericucumissativusil AT duolv mutationinicsyl21iencodingaplastidisoformoftriosephosphateisomeraseleadstoiyellowleaf21iiyl21iincucumbericucumissativusil AT huanlehe mutationinicsyl21iencodingaplastidisoformoftriosephosphateisomeraseleadstoiyellowleaf21iiyl21iincucumbericucumissativusil AT junsongpan mutationinicsyl21iencodingaplastidisoformoftriosephosphateisomeraseleadstoiyellowleaf21iiyl21iincucumbericucumissativusil AT runcai mutationinicsyl21iencodingaplastidisoformoftriosephosphateisomeraseleadstoiyellowleaf21iiyl21iincucumbericucumissativusil AT gangwang mutationinicsyl21iencodingaplastidisoformoftriosephosphateisomeraseleadstoiyellowleaf21iiyl21iincucumbericucumissativusil |