Does Dietary Deoxynivalenol Modulate the Acute Phase Reaction in Endotoxaemic Pigs?—Lessons from Clinical Signs, White Blood Cell Counts, and TNF-Alpha
We studied the interaction between deoxynivalenol (DON)-feeding and a subsequent pre- and post-hepatic immune stimulus with the hypothesis that the liver differently mediates the acute phase reaction (APR) in pigs. Barrows (n = 44) were divided into a DON-(4.59 mg DON/kg feed) and a control-diet gro...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-12-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/8/1/3 |
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| author | Tanja Tesch Erik Bannert Jeannette Kluess Jana Frahm Susanne Kersten Gerhard Breves Lydia Renner Stefan Kahlert Hermann-Josef Rothkötter Sven Dänicke |
| author_facet | Tanja Tesch Erik Bannert Jeannette Kluess Jana Frahm Susanne Kersten Gerhard Breves Lydia Renner Stefan Kahlert Hermann-Josef Rothkötter Sven Dänicke |
| author_sort | Tanja Tesch |
| collection | DOAJ |
| description | We studied the interaction between deoxynivalenol (DON)-feeding and a subsequent pre- and post-hepatic immune stimulus with the hypothesis that the liver differently mediates the acute phase reaction (APR) in pigs. Barrows (n = 44) were divided into a DON-(4.59 mg DON/kg feed) and a control-diet group, surgically equipped with permanent catheters pre- (V. portae hepatis) and post-hepatic (V. jugularis interna) and infused either with 0.9% NaCl or LPS (7.5 µg/kg BW). Thus, combination of diet (CON vs. DON) and infusion (CON vs. LPS, jugular vs. portal) created six groups: CON_CONjug.-CONpor., CON_CONjug.-LPSpor., CON_LPSjug.-CONpor., DON_CONjug.-CONpor., DON_CONjug.-LPSpor., DON_LPSjug.-CONpor.. Blood samples were taken at −30, 15, 30, 45, 60, 75, 90, 120, 150, 180 min relative to infusion and analyzed for leukocytes and TNF-alpha. Concurrently, clinical signs were scored and body temperature measured during the same period. LPS as such induced a dramatic rise in TNF-alpha (p < 0.001), hyperthermia (p < 0.01), and severe leukopenia (p < 0.001). In CON-fed pigs, an earlier return to physiological base levels was observed for the clinical complex, starting at 120 min post infusionem (p < 0.05) and persisting until 180 min. DON_LPSjug.-CONpor. resulted in a lower temperature rise (p = 0.08) compared to CON_LPSjug.-CONpor.. In conclusion, APR resulting from a post-hepatic immune stimulus was altered by chronic DON-feeding. |
| first_indexed | 2024-04-13T06:45:32Z |
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| id | doaj.art-363906293497485586c73bfb080ad77f |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-13T06:45:32Z |
| publishDate | 2015-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-363906293497485586c73bfb080ad77f2022-12-22T02:57:35ZengMDPI AGToxins2072-66512015-12-01813010.3390/toxins8010003toxins8010003Does Dietary Deoxynivalenol Modulate the Acute Phase Reaction in Endotoxaemic Pigs?—Lessons from Clinical Signs, White Blood Cell Counts, and TNF-AlphaTanja Tesch0Erik Bannert1Jeannette Kluess2Jana Frahm3Susanne Kersten4Gerhard Breves5Lydia Renner6Stefan Kahlert7Hermann-Josef Rothkötter8Sven Dänicke9Institute of Animal Nutrition, Friedrich-Loeffler Institute (FLI), Federal Research Institute of Animal Health, Bundesallee 50, 38116 Braunschweig, GermanyInstitute of Animal Nutrition, Friedrich-Loeffler Institute (FLI), Federal Research Institute of Animal Health, Bundesallee 50, 38116 Braunschweig, GermanyInstitute of Animal Nutrition, Friedrich-Loeffler Institute (FLI), Federal Research Institute of Animal Health, Bundesallee 50, 38116 Braunschweig, GermanyInstitute of Animal Nutrition, Friedrich-Loeffler Institute (FLI), Federal Research Institute of Animal Health, Bundesallee 50, 38116 Braunschweig, GermanyInstitute of Animal Nutrition, Friedrich-Loeffler Institute (FLI), Federal Research Institute of Animal Health, Bundesallee 50, 38116 Braunschweig, GermanyInstitute for Physiology, University of Veterinary Medicine, Foundation, Bischofsholer Damm 15, 30173 Hannover, GermanyInstitute of Anatomy, Otto von Guericke University Magdeburg, Leipziger Str. 44, 39120 Magdeburg, GermanyInstitute of Anatomy, Otto von Guericke University Magdeburg, Leipziger Str. 44, 39120 Magdeburg, GermanyInstitute of Anatomy, Otto von Guericke University Magdeburg, Leipziger Str. 44, 39120 Magdeburg, GermanyInstitute of Animal Nutrition, Friedrich-Loeffler Institute (FLI), Federal Research Institute of Animal Health, Bundesallee 50, 38116 Braunschweig, GermanyWe studied the interaction between deoxynivalenol (DON)-feeding and a subsequent pre- and post-hepatic immune stimulus with the hypothesis that the liver differently mediates the acute phase reaction (APR) in pigs. Barrows (n = 44) were divided into a DON-(4.59 mg DON/kg feed) and a control-diet group, surgically equipped with permanent catheters pre- (V. portae hepatis) and post-hepatic (V. jugularis interna) and infused either with 0.9% NaCl or LPS (7.5 µg/kg BW). Thus, combination of diet (CON vs. DON) and infusion (CON vs. LPS, jugular vs. portal) created six groups: CON_CONjug.-CONpor., CON_CONjug.-LPSpor., CON_LPSjug.-CONpor., DON_CONjug.-CONpor., DON_CONjug.-LPSpor., DON_LPSjug.-CONpor.. Blood samples were taken at −30, 15, 30, 45, 60, 75, 90, 120, 150, 180 min relative to infusion and analyzed for leukocytes and TNF-alpha. Concurrently, clinical signs were scored and body temperature measured during the same period. LPS as such induced a dramatic rise in TNF-alpha (p < 0.001), hyperthermia (p < 0.01), and severe leukopenia (p < 0.001). In CON-fed pigs, an earlier return to physiological base levels was observed for the clinical complex, starting at 120 min post infusionem (p < 0.05) and persisting until 180 min. DON_LPSjug.-CONpor. resulted in a lower temperature rise (p = 0.08) compared to CON_LPSjug.-CONpor.. In conclusion, APR resulting from a post-hepatic immune stimulus was altered by chronic DON-feeding.http://www.mdpi.com/2072-6651/8/1/3pigdeoxynivalenollipopolysaccharideliverleukocytesclinical symptomstumor necrosis factor alphabody core temperature |
| spellingShingle | Tanja Tesch Erik Bannert Jeannette Kluess Jana Frahm Susanne Kersten Gerhard Breves Lydia Renner Stefan Kahlert Hermann-Josef Rothkötter Sven Dänicke Does Dietary Deoxynivalenol Modulate the Acute Phase Reaction in Endotoxaemic Pigs?—Lessons from Clinical Signs, White Blood Cell Counts, and TNF-Alpha Toxins pig deoxynivalenol lipopolysaccharide liver leukocytes clinical symptoms tumor necrosis factor alpha body core temperature |
| title | Does Dietary Deoxynivalenol Modulate the Acute Phase Reaction in Endotoxaemic Pigs?—Lessons from Clinical Signs, White Blood Cell Counts, and TNF-Alpha |
| title_full | Does Dietary Deoxynivalenol Modulate the Acute Phase Reaction in Endotoxaemic Pigs?—Lessons from Clinical Signs, White Blood Cell Counts, and TNF-Alpha |
| title_fullStr | Does Dietary Deoxynivalenol Modulate the Acute Phase Reaction in Endotoxaemic Pigs?—Lessons from Clinical Signs, White Blood Cell Counts, and TNF-Alpha |
| title_full_unstemmed | Does Dietary Deoxynivalenol Modulate the Acute Phase Reaction in Endotoxaemic Pigs?—Lessons from Clinical Signs, White Blood Cell Counts, and TNF-Alpha |
| title_short | Does Dietary Deoxynivalenol Modulate the Acute Phase Reaction in Endotoxaemic Pigs?—Lessons from Clinical Signs, White Blood Cell Counts, and TNF-Alpha |
| title_sort | does dietary deoxynivalenol modulate the acute phase reaction in endotoxaemic pigs lessons from clinical signs white blood cell counts and tnf alpha |
| topic | pig deoxynivalenol lipopolysaccharide liver leukocytes clinical symptoms tumor necrosis factor alpha body core temperature |
| url | http://www.mdpi.com/2072-6651/8/1/3 |
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