Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles
Abstract The aim of this study was to investigate the effect of bioactive peptides (BAPT) from animal sources on the development of mouse preantral follicles in vitro. Preantral follicles were isolated and randomly divided into the following groups: an untreated group (control) and three groups supp...
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| Format: | Article |
| Language: | English |
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BMC
2020-09-01
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| Series: | Journal of Ovarian Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13048-020-00695-8 |
| _version_ | 1797966326786949120 |
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| author | Gang Liu Shubin Li Jinyu Ren Chunyu Wang Yaxuan Zhang Xiulan Su Yanfeng Dai |
| author_facet | Gang Liu Shubin Li Jinyu Ren Chunyu Wang Yaxuan Zhang Xiulan Su Yanfeng Dai |
| author_sort | Gang Liu |
| collection | DOAJ |
| description | Abstract The aim of this study was to investigate the effect of bioactive peptides (BAPT) from animal sources on the development of mouse preantral follicles in vitro. Preantral follicles were isolated and randomly divided into the following groups: an untreated group (control) and three groups supplemented with 20, 40 and 60 μg/mL BAPT, respectively. After establishing the in vitro follicle culture, the gene expression levels and hormone levels were quantified. After in vitro maturation, the developmental rates, reactive oxygen species (ROS) production levels and mitochondrial distributions of MII oocytes were investigated, followed by the analyses of embryonic developmental rates after in vitro fertilization. The results showed that BAPT promoted the growth of mouse preantral follicles. Notably, after 14 d of in vitro culture, the levels of 17 β-estradiol and progesterone were up-regulated with BAPT treatments. Moreover, the expression levels of Oct4, Bmp15, GDF9, FOXO3, Zp3, FOXL2, Inhibin alpha, SOD2, Catalase, GPx and Bcl-2 in the developing follicles were significantly up-regulated after BAPT treatments (P < 0.05), while BAPT significantly inhibited the expression levels of BAX (P < 0.05). Following BAPT treatments, the ROS production levels of MII oocytes were decreased while the mitochondrial distributions were significantly enhanced. Furthermore, increased maturation rates, fertilization and embryonic developmental rates were found in these BAPT-treated groups (P < 0.05). These results demonstrated that BAPT significantly improved the development of preantral follicles in vitro by reducing ROS-dependent cellular damages and by enhancing mitochondrial distributions, thereby promoting the further applications of animal-derived BAPT in biomedical research. |
| first_indexed | 2024-04-11T02:13:48Z |
| format | Article |
| id | doaj.art-363a9a4a98734b26b72a9034215de59e |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-04-11T02:13:48Z |
| publishDate | 2020-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-363a9a4a98734b26b72a9034215de59e2023-01-03T01:39:18ZengBMCJournal of Ovarian Research1757-22152020-09-0113111110.1186/s13048-020-00695-8Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral folliclesGang Liu0Shubin Li1Jinyu Ren2Chunyu Wang3Yaxuan Zhang4Xiulan Su5Yanfeng Dai6Key Laboratory of Medical Cell Biology, Clinical Medicine Research Center, Affiliated Hospital of Inner Mongolia Medical CollegeCollege of Life Science, Inner Mongolia UniversityCollege of Life Science, Inner Mongolia UniversityCollege of Life Science, Inner Mongolia UniversityCollege of Life Science, Inner Mongolia UniversityKey Laboratory of Medical Cell Biology, Clinical Medicine Research Center, Affiliated Hospital of Inner Mongolia Medical CollegeCollege of Life Science, Inner Mongolia UniversityAbstract The aim of this study was to investigate the effect of bioactive peptides (BAPT) from animal sources on the development of mouse preantral follicles in vitro. Preantral follicles were isolated and randomly divided into the following groups: an untreated group (control) and three groups supplemented with 20, 40 and 60 μg/mL BAPT, respectively. After establishing the in vitro follicle culture, the gene expression levels and hormone levels were quantified. After in vitro maturation, the developmental rates, reactive oxygen species (ROS) production levels and mitochondrial distributions of MII oocytes were investigated, followed by the analyses of embryonic developmental rates after in vitro fertilization. The results showed that BAPT promoted the growth of mouse preantral follicles. Notably, after 14 d of in vitro culture, the levels of 17 β-estradiol and progesterone were up-regulated with BAPT treatments. Moreover, the expression levels of Oct4, Bmp15, GDF9, FOXO3, Zp3, FOXL2, Inhibin alpha, SOD2, Catalase, GPx and Bcl-2 in the developing follicles were significantly up-regulated after BAPT treatments (P < 0.05), while BAPT significantly inhibited the expression levels of BAX (P < 0.05). Following BAPT treatments, the ROS production levels of MII oocytes were decreased while the mitochondrial distributions were significantly enhanced. Furthermore, increased maturation rates, fertilization and embryonic developmental rates were found in these BAPT-treated groups (P < 0.05). These results demonstrated that BAPT significantly improved the development of preantral follicles in vitro by reducing ROS-dependent cellular damages and by enhancing mitochondrial distributions, thereby promoting the further applications of animal-derived BAPT in biomedical research.http://link.springer.com/article/10.1186/s13048-020-00695-8Preantral folliclesBAPTROSEmbryonic development |
| spellingShingle | Gang Liu Shubin Li Jinyu Ren Chunyu Wang Yaxuan Zhang Xiulan Su Yanfeng Dai Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles Journal of Ovarian Research Preantral follicles BAPT ROS Embryonic development |
| title | Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles |
| title_full | Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles |
| title_fullStr | Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles |
| title_full_unstemmed | Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles |
| title_short | Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles |
| title_sort | effect of animal sourced bioactive peptides on the in vitro development of mouse preantral follicles |
| topic | Preantral follicles BAPT ROS Embryonic development |
| url | http://link.springer.com/article/10.1186/s13048-020-00695-8 |
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