Probiotic supplementation restores normal microbiota composition and function in antibiotic-treated and in caesarean-born infants
Abstract Background Infants born by caesarean section or receiving antibiotics are at increased risk of developing metabolic, inflammatory and immunological diseases, potentially due to disruption of normal gut microbiota at a critical developmental time window. We investigated whether probiotic sup...
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| Format: | Article |
| Language: | English |
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BMC
2018-10-01
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| Series: | Microbiome |
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| Online Access: | http://link.springer.com/article/10.1186/s40168-018-0567-4 |
| _version_ | 1818279218615681024 |
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| author | Katri Korpela Anne Salonen Outi Vepsäläinen Marjo Suomalainen Carolin Kolmeder Markku Varjosalo Sini Miettinen Kaarina Kukkonen Erkki Savilahti Mikael Kuitunen Willem M de Vos |
| author_facet | Katri Korpela Anne Salonen Outi Vepsäläinen Marjo Suomalainen Carolin Kolmeder Markku Varjosalo Sini Miettinen Kaarina Kukkonen Erkki Savilahti Mikael Kuitunen Willem M de Vos |
| author_sort | Katri Korpela |
| collection | DOAJ |
| description | Abstract Background Infants born by caesarean section or receiving antibiotics are at increased risk of developing metabolic, inflammatory and immunological diseases, potentially due to disruption of normal gut microbiota at a critical developmental time window. We investigated whether probiotic supplementation could ameliorate the effects of antibiotic use or caesarean birth on infant microbiota in a double blind, placebo-controlled randomized clinical trial. Mothers were given a multispecies probiotic, consisting of Bifidobacterium breve Bb99 (Bp99 2 × 108 cfu) Propionibacterium freundenreichii subsp. shermanii JS (2 × 109cfu), Lactobacillus rhamnosus Lc705 (5 × 109 cfu) and Lactobacillus rhamnosus GG (5 × 109 cfu) (N = 168 breastfed and 31 formula-fed), or placebo supplement (N = 201 breastfed and 22 formula-fed) during pregnancy, and the infants were given the same supplement. Faecal samples of the infants were collected at 3 months and analyzed using taxonomic, metagenomic and metaproteomic approaches. Results The probiotic supplement had a strong overall impact on the microbiota composition, but the effect depended on the infant’s diet. Only breastfed infants showed the expected increase in bifidobacteria and reduction in Proteobacteria and Clostridia. In the placebo group, both birth mode and antibiotic use were significantly associated with altered microbiota composition and function, particularly reduced Bifidobacterium abundance. In the probiotic group, the effects of antibiotics and birth mode were either completely eliminated or reduced. Conclusions The results indicate that it is possible to correct undesired changes in microbiota composition and function caused by antibiotic treatments or caesarean birth by supplementing infants with a probiotic mixture together with at least partial breastfeeding. Trial registration clinicaltrials.gov NCT00298337. Registered March 2, 2006. |
| first_indexed | 2024-12-12T23:29:50Z |
| format | Article |
| id | doaj.art-363b758cc2a34c6dbac8d831b1d8140c |
| institution | Directory Open Access Journal |
| issn | 2049-2618 |
| language | English |
| last_indexed | 2024-12-12T23:29:50Z |
| publishDate | 2018-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbiome |
| spelling | doaj.art-363b758cc2a34c6dbac8d831b1d8140c2022-12-22T00:07:51ZengBMCMicrobiome2049-26182018-10-016111110.1186/s40168-018-0567-4Probiotic supplementation restores normal microbiota composition and function in antibiotic-treated and in caesarean-born infantsKatri Korpela0Anne Salonen1Outi Vepsäläinen2Marjo Suomalainen3Carolin Kolmeder4Markku Varjosalo5Sini Miettinen6Kaarina Kukkonen7Erkki Savilahti8Mikael Kuitunen9Willem M de Vos10Immunobiology Research Programme, Department of Bacteriology and Immunology, University of HelsinkiImmunobiology Research Programme, Department of Bacteriology and Immunology, University of HelsinkiDepartment of Veterinary Biosciences, Faculty of Veterinary Medicine, University of HelsinkiDepartment of Veterinary Biosciences, Faculty of Veterinary Medicine, University of HelsinkiDepartment of Microbiome Science, Max Planck Institute for Developmental BiologyInstitute of Biotechnology, University of HelsinkiInstitute of Biotechnology, University of HelsinkiSkin and Allergy Hospital, Department of Paediatrics, Helsinki University HospitalChildren’s Hospital, University of Helsinki and Helsinki University HospitalChildren’s Hospital, University of Helsinki and Helsinki University HospitalImmunobiology Research Programme, Department of Bacteriology and Immunology, University of HelsinkiAbstract Background Infants born by caesarean section or receiving antibiotics are at increased risk of developing metabolic, inflammatory and immunological diseases, potentially due to disruption of normal gut microbiota at a critical developmental time window. We investigated whether probiotic supplementation could ameliorate the effects of antibiotic use or caesarean birth on infant microbiota in a double blind, placebo-controlled randomized clinical trial. Mothers were given a multispecies probiotic, consisting of Bifidobacterium breve Bb99 (Bp99 2 × 108 cfu) Propionibacterium freundenreichii subsp. shermanii JS (2 × 109cfu), Lactobacillus rhamnosus Lc705 (5 × 109 cfu) and Lactobacillus rhamnosus GG (5 × 109 cfu) (N = 168 breastfed and 31 formula-fed), or placebo supplement (N = 201 breastfed and 22 formula-fed) during pregnancy, and the infants were given the same supplement. Faecal samples of the infants were collected at 3 months and analyzed using taxonomic, metagenomic and metaproteomic approaches. Results The probiotic supplement had a strong overall impact on the microbiota composition, but the effect depended on the infant’s diet. Only breastfed infants showed the expected increase in bifidobacteria and reduction in Proteobacteria and Clostridia. In the placebo group, both birth mode and antibiotic use were significantly associated with altered microbiota composition and function, particularly reduced Bifidobacterium abundance. In the probiotic group, the effects of antibiotics and birth mode were either completely eliminated or reduced. Conclusions The results indicate that it is possible to correct undesired changes in microbiota composition and function caused by antibiotic treatments or caesarean birth by supplementing infants with a probiotic mixture together with at least partial breastfeeding. Trial registration clinicaltrials.gov NCT00298337. Registered March 2, 2006.http://link.springer.com/article/10.1186/s40168-018-0567-4Early-life microbiotaBifidobacteriaLactobacilliMetagenomicsMetaproteomics |
| spellingShingle | Katri Korpela Anne Salonen Outi Vepsäläinen Marjo Suomalainen Carolin Kolmeder Markku Varjosalo Sini Miettinen Kaarina Kukkonen Erkki Savilahti Mikael Kuitunen Willem M de Vos Probiotic supplementation restores normal microbiota composition and function in antibiotic-treated and in caesarean-born infants Microbiome Early-life microbiota Bifidobacteria Lactobacilli Metagenomics Metaproteomics |
| title | Probiotic supplementation restores normal microbiota composition and function in antibiotic-treated and in caesarean-born infants |
| title_full | Probiotic supplementation restores normal microbiota composition and function in antibiotic-treated and in caesarean-born infants |
| title_fullStr | Probiotic supplementation restores normal microbiota composition and function in antibiotic-treated and in caesarean-born infants |
| title_full_unstemmed | Probiotic supplementation restores normal microbiota composition and function in antibiotic-treated and in caesarean-born infants |
| title_short | Probiotic supplementation restores normal microbiota composition and function in antibiotic-treated and in caesarean-born infants |
| title_sort | probiotic supplementation restores normal microbiota composition and function in antibiotic treated and in caesarean born infants |
| topic | Early-life microbiota Bifidobacteria Lactobacilli Metagenomics Metaproteomics |
| url | http://link.springer.com/article/10.1186/s40168-018-0567-4 |
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