Ethanol extract of Poria cocos induces apoptosis and differentiation in Neuro-2a neuroblastoma cells
Poria cocos is a widely used ingredient in traditional Chinese medicine and dietary supplement. In this study, the effects of the ethanol extract of P. cocos on cell proliferation in Neuro-2a neuroblastoma cells and its possible mechanism of action are investigated. The extract inhibited the growth...
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| Format: | Article |
| Language: | English |
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Bangladesh Pharmacological Society
2022-11-01
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| Series: | Bangladesh Journal of Pharmacology |
| Subjects: | |
| Online Access: | https://www.banglajol.info/index.php/BJP/article/view/57734 |
| _version_ | 1811205514042802176 |
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| author | Xia Jiang Jie Xie Zhaotun Hu Xiaoliang Xiang |
| author_facet | Xia Jiang Jie Xie Zhaotun Hu Xiaoliang Xiang |
| author_sort | Xia Jiang |
| collection | DOAJ |
| description |
Poria cocos is a widely used ingredient in traditional Chinese medicine and dietary supplement. In this study, the effects of the ethanol extract of P. cocos on cell proliferation in Neuro-2a neuroblastoma cells and its possible mechanism of action are investigated. The extract inhibited the growth of Neuro-2a cells in a dose-dependent manner with an IC50 value of 34.6 μg/mL. To further elucidate the mechanism by which the extract acts, transcriptome analysis using high-throughput Illumina RNA-seq technology was performed. Transcriptome analysis revealed that the cell cycle as the potential primary target pathway of the extract in Neuro-2a cells. Flow cytometry analysis confirmed that the extract induced cell cycle arrest at G1 phase. Furthermore, the extract also induced apoptosis in Neuro-2a cells. Notably, it was found that Neuro-2a cells exhibited morphological features of differentiation when treated with the extract. This study suggests that P. cocos may be a potential candidate therapeutic agent in combating neuroblastoma.
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| first_indexed | 2024-04-12T03:33:04Z |
| format | Article |
| id | doaj.art-363d3dbc97fe4f668e239546dfb05e8e |
| institution | Directory Open Access Journal |
| issn | 1991-0088 |
| language | English |
| last_indexed | 2024-04-12T03:33:04Z |
| publishDate | 2022-11-01 |
| publisher | Bangladesh Pharmacological Society |
| record_format | Article |
| series | Bangladesh Journal of Pharmacology |
| spelling | doaj.art-363d3dbc97fe4f668e239546dfb05e8e2022-12-22T03:49:30ZengBangladesh Pharmacological SocietyBangladesh Journal of Pharmacology1991-00882022-11-0117410.3329/bjp.v17i4.57734Ethanol extract of Poria cocos induces apoptosis and differentiation in Neuro-2a neuroblastoma cellsXia Jiang0Jie Xie1Zhaotun Hu2Xiaoliang Xiang3Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province，College of Biological and Food Engineering, Huaihua University, Huaihua, China.Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province，College of Biological and Food Engineering, Huaihua University, Huaihua, China.Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province，College of Biological and Food Engineering, Huaihua University, Huaihua, China.Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province，College of Biological and Food Engineering, Huaihua University, Huaihua, China. Poria cocos is a widely used ingredient in traditional Chinese medicine and dietary supplement. In this study, the effects of the ethanol extract of P. cocos on cell proliferation in Neuro-2a neuroblastoma cells and its possible mechanism of action are investigated. The extract inhibited the growth of Neuro-2a cells in a dose-dependent manner with an IC50 value of 34.6 μg/mL. To further elucidate the mechanism by which the extract acts, transcriptome analysis using high-throughput Illumina RNA-seq technology was performed. Transcriptome analysis revealed that the cell cycle as the potential primary target pathway of the extract in Neuro-2a cells. Flow cytometry analysis confirmed that the extract induced cell cycle arrest at G1 phase. Furthermore, the extract also induced apoptosis in Neuro-2a cells. Notably, it was found that Neuro-2a cells exhibited morphological features of differentiation when treated with the extract. This study suggests that P. cocos may be a potential candidate therapeutic agent in combating neuroblastoma. https://www.banglajol.info/index.php/BJP/article/view/57734ApoptosisCell differentiationNeuroblastomaNeuro-2aPoria cocos |
| spellingShingle | Xia Jiang Jie Xie Zhaotun Hu Xiaoliang Xiang Ethanol extract of Poria cocos induces apoptosis and differentiation in Neuro-2a neuroblastoma cells Bangladesh Journal of Pharmacology Apoptosis Cell differentiation Neuroblastoma Neuro-2a Poria cocos |
| title | Ethanol extract of Poria cocos induces apoptosis and differentiation in Neuro-2a neuroblastoma cells |
| title_full | Ethanol extract of Poria cocos induces apoptosis and differentiation in Neuro-2a neuroblastoma cells |
| title_fullStr | Ethanol extract of Poria cocos induces apoptosis and differentiation in Neuro-2a neuroblastoma cells |
| title_full_unstemmed | Ethanol extract of Poria cocos induces apoptosis and differentiation in Neuro-2a neuroblastoma cells |
| title_short | Ethanol extract of Poria cocos induces apoptosis and differentiation in Neuro-2a neuroblastoma cells |
| title_sort | ethanol extract of poria cocos induces apoptosis and differentiation in neuro 2a neuroblastoma cells |
| topic | Apoptosis Cell differentiation Neuroblastoma Neuro-2a Poria cocos |
| url | https://www.banglajol.info/index.php/BJP/article/view/57734 |
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