Role of leptin up-regulated GRP78 expression in migration of lung cancer cells
Objective To investigate the effect of leptin on the migration of lung cancer cell lines A549 and H460 and the potential mechanisms. Methods The GEPIA website was used to analyze the correlation of the expression levels of leptin and glucose-regulated protein-78 (GRP78) with overall survival rate in...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Third Military Medical University
2020-04-01
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| Series: | Di-san junyi daxue xuebao |
| Subjects: | |
| Online Access: | http://aammt.tmmu.edu.cn/Upload/rhtml/201911165.htm |
| _version_ | 1830351929543229440 |
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| author | WU Youqile WU Youqile LIN Xiaojing WU Jingjing LIAN Xuemei |
| author_facet | WU Youqile WU Youqile LIN Xiaojing WU Jingjing LIAN Xuemei |
| author_sort | WU Youqile |
| collection | DOAJ |
| description | Objective To investigate the effect of leptin on the migration of lung cancer cell lines A549 and H460 and the potential mechanisms. Methods The GEPIA website was used to analyze the correlation of the expression levels of leptin and glucose-regulated protein-78 (GRP78) with overall survival rate in lung cancer patients from TCGA database. Wound healing assay was used to detect the effect of leptin and HA15 (GRP78 targeting inhibitor) on the migration of lung cancer A549 and H460 cells; Immunofluorescence assay was used to measure the expression of GRP78 in lung cancer cells. Finally, lung cancer A549 cells were treated with PI3K targeting inhibitor Ly294002, mTOR targeting inhibitor rapamycin alone or in combination with leptin, and then Western blot assay was used to investigate the impact of leptin on PI3K/mTOR/STAT3 signaling pathway and GRP78 expression. Results Survival analysis showed that high expression of leptin or GRP78 was closely related to decreased overall survival rate in lung cancer patients (P < 0.05). Wound healing test indicated that leptin enhanced the migration of lung cancer A549 and H460 cells (P < 0.05). Immunofluorescence assay displayed that leptin upregulated GRP78 expression in A549 and H460 cells (P < 0.01). Western blot assay suggested that leptin could upregulate the expression of GRP78 in lung cancer A549 cells through PI3K/mTOR/STAT3 signaling pathway (P < 0.001). Wound healing test showed inhibition of GRP78 attenuated the effect of leptin on promoting migration. Conclusion Leptin promotes the migration of lung cancer cells by up-regulating GRP78 through PI3K/mTOR/STAT3 signaling pathway. |
| first_indexed | 2024-12-20T00:47:00Z |
| format | Article |
| id | doaj.art-363fd3d90dca43cfa36bb37b319c5360 |
| institution | Directory Open Access Journal |
| issn | 1000-5404 |
| language | zho |
| last_indexed | 2024-12-20T00:47:00Z |
| publishDate | 2020-04-01 |
| publisher | Editorial Office of Journal of Third Military Medical University |
| record_format | Article |
| series | Di-san junyi daxue xuebao |
| spelling | doaj.art-363fd3d90dca43cfa36bb37b319c53602022-12-21T19:59:22ZzhoEditorial Office of Journal of Third Military Medical UniversityDi-san junyi daxue xuebao1000-54042020-04-0142879980610.16016/j.1000-5404.201911165Role of leptin up-regulated GRP78 expression in migration of lung cancer cellsWU Youqile0WU Youqile1LIN Xiaojing2WU Jingjing3LIAN Xuemei4Chongqing Key Laboratory for Lipid and Glucose Metabolic Diseases, Key Laboratory of Molecular Biology for Infectious Diseases of Ministry of Education, Chongqing, 400016, ChinaDepartment of Nutrition and Food Hygiene, School of Public Health and Management, Chongqing Medical University, Chongqing, 400016, China Key Laboratory of Translational Medicine in Major Metabolic Diseases, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China Chongqing Key Laboratory for Lipid and Glucose Metabolic Diseases, Key Laboratory of Molecular Biology for Infectious Diseases of Ministry of Education, Chongqing, 400016, ChinaChongqing Key Laboratory for Lipid and Glucose Metabolic Diseases, Key Laboratory of Molecular Biology for Infectious Diseases of Ministry of Education, Chongqing, 400016, ChinaObjective To investigate the effect of leptin on the migration of lung cancer cell lines A549 and H460 and the potential mechanisms. Methods The GEPIA website was used to analyze the correlation of the expression levels of leptin and glucose-regulated protein-78 (GRP78) with overall survival rate in lung cancer patients from TCGA database. Wound healing assay was used to detect the effect of leptin and HA15 (GRP78 targeting inhibitor) on the migration of lung cancer A549 and H460 cells; Immunofluorescence assay was used to measure the expression of GRP78 in lung cancer cells. Finally, lung cancer A549 cells were treated with PI3K targeting inhibitor Ly294002, mTOR targeting inhibitor rapamycin alone or in combination with leptin, and then Western blot assay was used to investigate the impact of leptin on PI3K/mTOR/STAT3 signaling pathway and GRP78 expression. Results Survival analysis showed that high expression of leptin or GRP78 was closely related to decreased overall survival rate in lung cancer patients (P < 0.05). Wound healing test indicated that leptin enhanced the migration of lung cancer A549 and H460 cells (P < 0.05). Immunofluorescence assay displayed that leptin upregulated GRP78 expression in A549 and H460 cells (P < 0.01). Western blot assay suggested that leptin could upregulate the expression of GRP78 in lung cancer A549 cells through PI3K/mTOR/STAT3 signaling pathway (P < 0.001). Wound healing test showed inhibition of GRP78 attenuated the effect of leptin on promoting migration. Conclusion Leptin promotes the migration of lung cancer cells by up-regulating GRP78 through PI3K/mTOR/STAT3 signaling pathway.http://aammt.tmmu.edu.cn/Upload/rhtml/201911165.htmleptinlung cancerglucose-regulated protein-78migration |
| spellingShingle | WU Youqile WU Youqile LIN Xiaojing WU Jingjing LIAN Xuemei Role of leptin up-regulated GRP78 expression in migration of lung cancer cells Di-san junyi daxue xuebao leptin lung cancer glucose-regulated protein-78 migration |
| title | Role of leptin up-regulated GRP78 expression in migration of lung cancer cells |
| title_full | Role of leptin up-regulated GRP78 expression in migration of lung cancer cells |
| title_fullStr | Role of leptin up-regulated GRP78 expression in migration of lung cancer cells |
| title_full_unstemmed | Role of leptin up-regulated GRP78 expression in migration of lung cancer cells |
| title_short | Role of leptin up-regulated GRP78 expression in migration of lung cancer cells |
| title_sort | role of leptin up regulated grp78 expression in migration of lung cancer cells |
| topic | leptin lung cancer glucose-regulated protein-78 migration |
| url | http://aammt.tmmu.edu.cn/Upload/rhtml/201911165.htm |
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