Cell culture models for SARS-CoV-2 infectivity and systemic complications
COVID-19 was declared by WHO as a pandemic since March 2020. The vaccination program has been implemented worldwide. Specific antiviral drugs such as remdesivir, molnupiravir and ritonavir-based nirmatrelvir were effective against SARS-CoV-2 infection. However, the new SARS-CoV-2 variants have been...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Pharmacy, Mahidol University
2022-07-01
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| Series: | Pharmaceutical Sciences Asia |
| Subjects: | |
| Online Access: | https://pharmacy.mahidol.ac.th/journal/_files/2022-49-4_1.pdf |
| _version_ | 1828369387672829952 |
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| author | Khanit Sa-ngiamsuntorn Piyanoot Thongsri Yongyut Pewkliang Adisak Wongkajornsilp |
| author_facet | Khanit Sa-ngiamsuntorn Piyanoot Thongsri Yongyut Pewkliang Adisak Wongkajornsilp |
| author_sort | Khanit Sa-ngiamsuntorn |
| collection | DOAJ |
| description | COVID-19 was declared by WHO as a pandemic since March 2020. The vaccination program has been implemented worldwide. Specific antiviral drugs such as remdesivir, molnupiravir and ritonavir-based nirmatrelvir were effective against SARS-CoV-2 infection. However, the new SARS-CoV-2 variants have been elevated due to viral mutation causing vaccine resistance and rapid spreading. Long-term COVID-19 complications have been life-threatening in some recovery cases. To overcome viral adaptation, cell culture model is essential to comprehend SARS-CoV-2 infection, pathophysiology, complications, and drug target alterations. The classical 2D culture cell was frequency used for viral propagation and high-throughput screening. Modern 3D culture has recapitulated key cellular and molecular events of tissue physiology. Here, we reviewed the cell lines, 3D culture, organoid and relevant models for the aforementioned applications. |
| first_indexed | 2024-04-14T06:23:14Z |
| format | Article |
| id | doaj.art-36410bc13caf43cc97a10291b2ae1119 |
| institution | Directory Open Access Journal |
| issn | 2586-8470 |
| language | English |
| last_indexed | 2024-04-14T06:23:14Z |
| publishDate | 2022-07-01 |
| publisher | Faculty of Pharmacy, Mahidol University |
| record_format | Article |
| series | Pharmaceutical Sciences Asia |
| spelling | doaj.art-36410bc13caf43cc97a10291b2ae11192022-12-22T02:07:56ZengFaculty of Pharmacy, Mahidol UniversityPharmaceutical Sciences Asia2586-84702022-07-0149429230310.29090/psa.2022.04.22.058Cell culture models for SARS-CoV-2 infectivity and systemic complicationsKhanit Sa-ngiamsuntorn0Piyanoot Thongsri1Yongyut Pewkliang2Adisak Wongkajornsilp3 Department of Biochemistry, Faculty of Pharmacy, Mahidol University, Bangkok, ThailandSection for Translational Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, ThailandSection for Translational Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, ThailandDepartment of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandCOVID-19 was declared by WHO as a pandemic since March 2020. The vaccination program has been implemented worldwide. Specific antiviral drugs such as remdesivir, molnupiravir and ritonavir-based nirmatrelvir were effective against SARS-CoV-2 infection. However, the new SARS-CoV-2 variants have been elevated due to viral mutation causing vaccine resistance and rapid spreading. Long-term COVID-19 complications have been life-threatening in some recovery cases. To overcome viral adaptation, cell culture model is essential to comprehend SARS-CoV-2 infection, pathophysiology, complications, and drug target alterations. The classical 2D culture cell was frequency used for viral propagation and high-throughput screening. Modern 3D culture has recapitulated key cellular and molecular events of tissue physiology. Here, we reviewed the cell lines, 3D culture, organoid and relevant models for the aforementioned applications.https://pharmacy.mahidol.ac.th/journal/_files/2022-49-4_1.pdfsars-cov-2cell culture modelcovid-193d cultureorganoid |
| spellingShingle | Khanit Sa-ngiamsuntorn Piyanoot Thongsri Yongyut Pewkliang Adisak Wongkajornsilp Cell culture models for SARS-CoV-2 infectivity and systemic complications Pharmaceutical Sciences Asia sars-cov-2 cell culture model covid-19 3d culture organoid |
| title | Cell culture models for SARS-CoV-2 infectivity and systemic complications |
| title_full | Cell culture models for SARS-CoV-2 infectivity and systemic complications |
| title_fullStr | Cell culture models for SARS-CoV-2 infectivity and systemic complications |
| title_full_unstemmed | Cell culture models for SARS-CoV-2 infectivity and systemic complications |
| title_short | Cell culture models for SARS-CoV-2 infectivity and systemic complications |
| title_sort | cell culture models for sars cov 2 infectivity and systemic complications |
| topic | sars-cov-2 cell culture model covid-19 3d culture organoid |
| url | https://pharmacy.mahidol.ac.th/journal/_files/2022-49-4_1.pdf |
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