The putative Cationic Amino acid Transporter 9 is targeted to vesicles and may be involved in plant amino acid homeostasis
Amino acids are major primary metabolites. Their uptake, translocation, compartmentation and re-mobilization require a diverse set of cellular transporters. Here, the broadly expressed gene product of CATIONIC AMINO ACID TRANSPORTER 9 (CAT9) was identified as mainly localized to vesicular membranes...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2015-04-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00212/full |
| _version_ | 1828869924314939392 |
|---|---|
| author | Huaiyu eYang York eStierhof Uwe eLudewig |
| author_facet | Huaiyu eYang York eStierhof Uwe eLudewig |
| author_sort | Huaiyu eYang |
| collection | DOAJ |
| description | Amino acids are major primary metabolites. Their uptake, translocation, compartmentation and re-mobilization require a diverse set of cellular transporters. Here, the broadly expressed gene product of CATIONIC AMINO ACID TRANSPORTER 9 (CAT9) was identified as mainly localized to vesicular membranes that are involved in vacuolar trafficking, including those of the trans-Golgi network. In order to probe whether and how these compartments are involved in amino acid homeostasis, a loss-of-function cat9-1 mutant and ectopic over-expressor plants were isolated. Under restricted nitrogen supply in soil, cat9-1 showed a chlorotic phenotype, which was reversed in the over-expressors. The total soluble amino acid pools were affected in the mutants, but this was only significant under poor nitrogen supply. Upon nitrogen starvation, the major soluble amino acid leaf pools decreased. This decrease was lower in cat9-1 and augmented in the over-expressor. Over-expression generally affected total soluble amino acid concentrations and finally improved the survival upon severe nitrogen starvation. The results potentially identify a novel function of vesicular amino acid transport mediated by CAT9 in the cellular nitrogen-dependent amino acid homeostasis. |
| first_indexed | 2024-12-13T06:00:53Z |
| format | Article |
| id | doaj.art-3641ca3f181344b185ed7e05626c68fa |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-13T06:00:53Z |
| publishDate | 2015-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-3641ca3f181344b185ed7e05626c68fa2022-12-21T23:57:21ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2015-04-01610.3389/fpls.2015.00212131925The putative Cationic Amino acid Transporter 9 is targeted to vesicles and may be involved in plant amino acid homeostasisHuaiyu eYang0York eStierhof1Uwe eLudewig2University of HohenheimCenter for Molecular Biology of PlantsUniversity of HohenheimAmino acids are major primary metabolites. Their uptake, translocation, compartmentation and re-mobilization require a diverse set of cellular transporters. Here, the broadly expressed gene product of CATIONIC AMINO ACID TRANSPORTER 9 (CAT9) was identified as mainly localized to vesicular membranes that are involved in vacuolar trafficking, including those of the trans-Golgi network. In order to probe whether and how these compartments are involved in amino acid homeostasis, a loss-of-function cat9-1 mutant and ectopic over-expressor plants were isolated. Under restricted nitrogen supply in soil, cat9-1 showed a chlorotic phenotype, which was reversed in the over-expressors. The total soluble amino acid pools were affected in the mutants, but this was only significant under poor nitrogen supply. Upon nitrogen starvation, the major soluble amino acid leaf pools decreased. This decrease was lower in cat9-1 and augmented in the over-expressor. Over-expression generally affected total soluble amino acid concentrations and finally improved the survival upon severe nitrogen starvation. The results potentially identify a novel function of vesicular amino acid transport mediated by CAT9 in the cellular nitrogen-dependent amino acid homeostasis.http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00212/fulltrans-Golgi NetworkAmino acid permeasenitrogen.Cationic amino acidprevacuole |
| spellingShingle | Huaiyu eYang York eStierhof Uwe eLudewig The putative Cationic Amino acid Transporter 9 is targeted to vesicles and may be involved in plant amino acid homeostasis Frontiers in Plant Science trans-Golgi Network Amino acid permease nitrogen. Cationic amino acid prevacuole |
| title | The putative Cationic Amino acid Transporter 9 is targeted to vesicles and may be involved in plant amino acid homeostasis |
| title_full | The putative Cationic Amino acid Transporter 9 is targeted to vesicles and may be involved in plant amino acid homeostasis |
| title_fullStr | The putative Cationic Amino acid Transporter 9 is targeted to vesicles and may be involved in plant amino acid homeostasis |
| title_full_unstemmed | The putative Cationic Amino acid Transporter 9 is targeted to vesicles and may be involved in plant amino acid homeostasis |
| title_short | The putative Cationic Amino acid Transporter 9 is targeted to vesicles and may be involved in plant amino acid homeostasis |
| title_sort | putative cationic amino acid transporter 9 is targeted to vesicles and may be involved in plant amino acid homeostasis |
| topic | trans-Golgi Network Amino acid permease nitrogen. Cationic amino acid prevacuole |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00212/full |
| work_keys_str_mv | AT huaiyueyang theputativecationicaminoacidtransporter9istargetedtovesiclesandmaybeinvolvedinplantaminoacidhomeostasis AT yorkestierhof theputativecationicaminoacidtransporter9istargetedtovesiclesandmaybeinvolvedinplantaminoacidhomeostasis AT uweeludewig theputativecationicaminoacidtransporter9istargetedtovesiclesandmaybeinvolvedinplantaminoacidhomeostasis AT huaiyueyang putativecationicaminoacidtransporter9istargetedtovesiclesandmaybeinvolvedinplantaminoacidhomeostasis AT yorkestierhof putativecationicaminoacidtransporter9istargetedtovesiclesandmaybeinvolvedinplantaminoacidhomeostasis AT uweeludewig putativecationicaminoacidtransporter9istargetedtovesiclesandmaybeinvolvedinplantaminoacidhomeostasis |