Association of Chlamydia trachomatis, C. pneumoniae, and IL-6 and IL-8 Gene Alterations With Heart Diseases
Atherosclerosis is a progressive disease characterized by chronic inflammation of the arterial walls, associated with genetic and infectious factors. The present study investigated the involvement of Chlamydia trachomatis and Chlamydia pneumoniae infections and immunological markers (C-reactive prot...
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Frontiers Media S.A.
2019-02-01
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Series: | Frontiers in Immunology |
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Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2019.00087/full |
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author | Nubia Caroline Costa Almeida Maria Alice Freitas Queiroz Sandra Souza Lima Igor Brasil Costa Marco Antonio Ayin Fossa Antonio Carlos R. Vallinoto Marluísa de Oliveira Guimarães Ishak Ricardo Ishak |
author_facet | Nubia Caroline Costa Almeida Maria Alice Freitas Queiroz Sandra Souza Lima Igor Brasil Costa Marco Antonio Ayin Fossa Antonio Carlos R. Vallinoto Marluísa de Oliveira Guimarães Ishak Ricardo Ishak |
author_sort | Nubia Caroline Costa Almeida |
collection | DOAJ |
description | Atherosclerosis is a progressive disease characterized by chronic inflammation of the arterial walls, associated with genetic and infectious factors. The present study investigated the involvement of Chlamydia trachomatis and Chlamydia pneumoniae infections and immunological markers (C-reactive protein, CRP, TNF-α, IL-6, IL-8, and IL-10) in the process of atherosclerosis. The evaluation included 159 patients for surgical revascularization (CAD) and 71 patients for surgical heart valve disease (HVD) at three hospitals in Belém, Brazil. The control group (CG) comprised 300 healthy individuals. Blood samples collected before surgery were used for antibodies detection (enzyme immunoassay), CRP (immunoturbidimetry) and IL-6 levels (enzyme immunoassay). Tissue fragments (atheroma plaque, heart valve and ascending aorta) were collected during surgery and subjected to qPCR for detection of bacterial DNA. Promoter region polymorphisms of each marker and relative quantification of TNF-α, IL-8, and IL-10 gene expression were performed. Demography and social information were similar to the general population involved with both diseases. Antibody prevalence to C. trachomatis was 30.6, 20.3, and 36.7% (in the CAD, HVD, and CG, respectively) and to C. pneumoniae was 83.6, 84.5, and 80.3% (in the CAD, HVD, and CG, respectively). C. trachomatis cryptic plasmid DNA was detected in 7.4% of the samples. Frequency of IL6−174G>C polymorphism was higher in CAD and HVD than in CG regardless of previous exposure to Chlamydia. Previous C. trachomatis infection showed involvement in HVD and CAD. Significant association between disease and previous C. pneumoniae infection was found only among HVD. GG genotype of IL6−174G>C is apparently a risk factor for heart disease, whereas AT genotype of IL8−251A>T was mainly involved in valvulopathies, including patients with prior exposure to C. pneumoniae. |
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issn | 1664-3224 |
language | English |
last_indexed | 2024-12-14T14:09:38Z |
publishDate | 2019-02-01 |
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spelling | doaj.art-36430c9687e44bc38d762abce21113422022-12-21T22:58:19ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-02-011010.3389/fimmu.2019.00087364637Association of Chlamydia trachomatis, C. pneumoniae, and IL-6 and IL-8 Gene Alterations With Heart DiseasesNubia Caroline Costa Almeida0Maria Alice Freitas Queiroz1Sandra Souza Lima2Igor Brasil Costa3Marco Antonio Ayin Fossa4Antonio Carlos R. Vallinoto5Marluísa de Oliveira Guimarães Ishak6Ricardo Ishak7Virus Laboratory, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilVirus Laboratory, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilVirus Laboratory, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilVirology Section, Evandro Chagas Institute, Ananindeua, BrazilCardiology Unit, Portuguese Beneficent Hospital, Belém, BrazilVirus Laboratory, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilVirus Laboratory, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilVirus Laboratory, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilAtherosclerosis is a progressive disease characterized by chronic inflammation of the arterial walls, associated with genetic and infectious factors. The present study investigated the involvement of Chlamydia trachomatis and Chlamydia pneumoniae infections and immunological markers (C-reactive protein, CRP, TNF-α, IL-6, IL-8, and IL-10) in the process of atherosclerosis. The evaluation included 159 patients for surgical revascularization (CAD) and 71 patients for surgical heart valve disease (HVD) at three hospitals in Belém, Brazil. The control group (CG) comprised 300 healthy individuals. Blood samples collected before surgery were used for antibodies detection (enzyme immunoassay), CRP (immunoturbidimetry) and IL-6 levels (enzyme immunoassay). Tissue fragments (atheroma plaque, heart valve and ascending aorta) were collected during surgery and subjected to qPCR for detection of bacterial DNA. Promoter region polymorphisms of each marker and relative quantification of TNF-α, IL-8, and IL-10 gene expression were performed. Demography and social information were similar to the general population involved with both diseases. Antibody prevalence to C. trachomatis was 30.6, 20.3, and 36.7% (in the CAD, HVD, and CG, respectively) and to C. pneumoniae was 83.6, 84.5, and 80.3% (in the CAD, HVD, and CG, respectively). C. trachomatis cryptic plasmid DNA was detected in 7.4% of the samples. Frequency of IL6−174G>C polymorphism was higher in CAD and HVD than in CG regardless of previous exposure to Chlamydia. Previous C. trachomatis infection showed involvement in HVD and CAD. Significant association between disease and previous C. pneumoniae infection was found only among HVD. GG genotype of IL6−174G>C is apparently a risk factor for heart disease, whereas AT genotype of IL8−251A>T was mainly involved in valvulopathies, including patients with prior exposure to C. pneumoniae.https://www.frontiersin.org/article/10.3389/fimmu.2019.00087/fullChlamydia pneumoniaeC. trachomatispolymorphismsIL-6IL-8IL-10 |
spellingShingle | Nubia Caroline Costa Almeida Maria Alice Freitas Queiroz Sandra Souza Lima Igor Brasil Costa Marco Antonio Ayin Fossa Antonio Carlos R. Vallinoto Marluísa de Oliveira Guimarães Ishak Ricardo Ishak Association of Chlamydia trachomatis, C. pneumoniae, and IL-6 and IL-8 Gene Alterations With Heart Diseases Frontiers in Immunology Chlamydia pneumoniae C. trachomatis polymorphisms IL-6 IL-8 IL-10 |
title | Association of Chlamydia trachomatis, C. pneumoniae, and IL-6 and IL-8 Gene Alterations With Heart Diseases |
title_full | Association of Chlamydia trachomatis, C. pneumoniae, and IL-6 and IL-8 Gene Alterations With Heart Diseases |
title_fullStr | Association of Chlamydia trachomatis, C. pneumoniae, and IL-6 and IL-8 Gene Alterations With Heart Diseases |
title_full_unstemmed | Association of Chlamydia trachomatis, C. pneumoniae, and IL-6 and IL-8 Gene Alterations With Heart Diseases |
title_short | Association of Chlamydia trachomatis, C. pneumoniae, and IL-6 and IL-8 Gene Alterations With Heart Diseases |
title_sort | association of chlamydia trachomatis c pneumoniae and il 6 and il 8 gene alterations with heart diseases |
topic | Chlamydia pneumoniae C. trachomatis polymorphisms IL-6 IL-8 IL-10 |
url | https://www.frontiersin.org/article/10.3389/fimmu.2019.00087/full |
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