Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure
Titanium alloy scaffolds with a porous structure have attracted much attention in bone defect repair. However, which pore structure is more beneficial to bone defect repair is controversial. In the present research, the Ti6Al4V alloy porous scaffolds with gradient pore sizes were designed and fabric...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2023.1190630/full |
| _version_ | 1827946185678127104 |
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| author | Chao Wang Chao Wang Jie Wu Jie Wu Leyi Liu Leyi Liu Duoling Xu Duoling Xu Yuanbo Liu Yuanbo Liu Shujun Li Wentao Hou Jian Wang Xun Chen Xun Chen Liyuan Sheng Huancai Lin Huancai Lin Dongsheng Yu Dongsheng Yu |
| author_facet | Chao Wang Chao Wang Jie Wu Jie Wu Leyi Liu Leyi Liu Duoling Xu Duoling Xu Yuanbo Liu Yuanbo Liu Shujun Li Wentao Hou Jian Wang Xun Chen Xun Chen Liyuan Sheng Huancai Lin Huancai Lin Dongsheng Yu Dongsheng Yu |
| author_sort | Chao Wang |
| collection | DOAJ |
| description | Titanium alloy scaffolds with a porous structure have attracted much attention in bone defect repair. However, which pore structure is more beneficial to bone defect repair is controversial. In the present research, the Ti6Al4V alloy porous scaffolds with gradient pore sizes were designed and fabricated. The microstructure characterization, tests of mechanical properties, and in vitro and in vivo experiments have been performed to systematically evaluate the effect of pore size on osteoinduction and osteogenesis. The results revealed that the contact angle with water, compressive strength, and elastic modulus of the Ti6Al4V alloy porous scaffolds decreased gradually with the increase of pore size. However, there were obvious drops when the pore size of the porous scaffold was around 600 μm. As the pore size increased, the proliferation and integrin β1 of RAW 264.7 macrophages seeded on Ti6Al4V alloy porous scaffolds increased at first, reaching a maximum value at a pore size of around 600 μm, and then decreased subsequently. The proliferation, integrin β1, and osteogenic gene-related expressions of Bone marrow mesenchymal stem cells (BMSCs) seeded on Ti6Al4V alloy porous scaffolds with different pore sizes all exhibited similar variations which rose with increased pore size firstly, obtaining the maximum value at pore size about 600 μm, and then declined. The in vivo experiments confirmed the in vitro results, and the Ti6Al4V alloy porous scaffold with a pore size of 600 μm possessed the better capability to induce new bone formation. Therefore, for the design of Ti6Al4V alloy with a regular porous scaffold, the surface morphology, porosity, strength, and elastic modulus should be considered systematically, which would determine the capability of osteoinduction and osteogenesis. |
| first_indexed | 2024-03-13T10:55:06Z |
| format | Article |
| id | doaj.art-36438d8511534a218a779816a7dca597 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-03-13T10:55:06Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-36438d8511534a218a779816a7dca5972023-05-17T04:42:41ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462023-05-011110.3389/fchem.2023.11906301190630Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structureChao Wang0Chao Wang1Jie Wu2Jie Wu3Leyi Liu4Leyi Liu5Duoling Xu6Duoling Xu7Yuanbo Liu8Yuanbo Liu9Shujun Li10Wentao Hou11Jian Wang12Xun Chen13Xun Chen14Liyuan Sheng15Huancai Lin16Huancai Lin17Dongsheng Yu18Dongsheng Yu19Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, ChinaHospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, ChinaHospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, ChinaHospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, ChinaHospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, ChinaInstitute of Metal Research, Chinese Academy of Sciences, Shenyang, ChinaInstitute of Metal Research, Chinese Academy of Sciences, Shenyang, ChinaShenzhen Institute, Peking University, Shenzhen, ChinaHospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, ChinaShenzhen Institute, Peking University, Shenzhen, ChinaHospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, ChinaHospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, ChinaTitanium alloy scaffolds with a porous structure have attracted much attention in bone defect repair. However, which pore structure is more beneficial to bone defect repair is controversial. In the present research, the Ti6Al4V alloy porous scaffolds with gradient pore sizes were designed and fabricated. The microstructure characterization, tests of mechanical properties, and in vitro and in vivo experiments have been performed to systematically evaluate the effect of pore size on osteoinduction and osteogenesis. The results revealed that the contact angle with water, compressive strength, and elastic modulus of the Ti6Al4V alloy porous scaffolds decreased gradually with the increase of pore size. However, there were obvious drops when the pore size of the porous scaffold was around 600 μm. As the pore size increased, the proliferation and integrin β1 of RAW 264.7 macrophages seeded on Ti6Al4V alloy porous scaffolds increased at first, reaching a maximum value at a pore size of around 600 μm, and then decreased subsequently. The proliferation, integrin β1, and osteogenic gene-related expressions of Bone marrow mesenchymal stem cells (BMSCs) seeded on Ti6Al4V alloy porous scaffolds with different pore sizes all exhibited similar variations which rose with increased pore size firstly, obtaining the maximum value at pore size about 600 μm, and then declined. The in vivo experiments confirmed the in vitro results, and the Ti6Al4V alloy porous scaffold with a pore size of 600 μm possessed the better capability to induce new bone formation. Therefore, for the design of Ti6Al4V alloy with a regular porous scaffold, the surface morphology, porosity, strength, and elastic modulus should be considered systematically, which would determine the capability of osteoinduction and osteogenesis.https://www.frontiersin.org/articles/10.3389/fchem.2023.1190630/fullporous scaffoldTi6Al4V alloypore structureosteogenesisbone defect repairing |
| spellingShingle | Chao Wang Chao Wang Jie Wu Jie Wu Leyi Liu Leyi Liu Duoling Xu Duoling Xu Yuanbo Liu Yuanbo Liu Shujun Li Wentao Hou Jian Wang Xun Chen Xun Chen Liyuan Sheng Huancai Lin Huancai Lin Dongsheng Yu Dongsheng Yu Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure Frontiers in Chemistry porous scaffold Ti6Al4V alloy pore structure osteogenesis bone defect repairing |
| title | Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure |
| title_full | Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure |
| title_fullStr | Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure |
| title_full_unstemmed | Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure |
| title_short | Improving osteoinduction and osteogenesis of Ti6Al4V alloy porous scaffold by regulating the pore structure |
| title_sort | improving osteoinduction and osteogenesis of ti6al4v alloy porous scaffold by regulating the pore structure |
| topic | porous scaffold Ti6Al4V alloy pore structure osteogenesis bone defect repairing |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2023.1190630/full |
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