Characterisation of antibodies for the immunochemical detection of Enterobacter sakazakii
An indirect competitive enzyme immunoassay of Enterobacter sakazakii has been developed. The rabbit polyclonal antibodies to heat-labile or heat-stable antigen of the type strain E. sakazakii CNCTC 5739T were prepared for these purposes. The detection limits of enzyme immunoassays were within the ra...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2009-12-01
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| Series: | Czech Journal of Food Sciences |
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| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-200911-0009_characterisation-of-antibodies-for-the-immunochemical-detection-of-enterobacter-sakazakii.php |
| _version_ | 1797899732818853888 |
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| author | Igor Hochel Jiří Škvor |
| author_facet | Igor Hochel Jiří Škvor |
| author_sort | Igor Hochel |
| collection | DOAJ |
| description | An indirect competitive enzyme immunoassay of Enterobacter sakazakii has been developed. The rabbit polyclonal antibodies to heat-labile or heat-stable antigen of the type strain E. sakazakii CNCTC 5739T were prepared for these purposes. The detection limits of enzyme immunoassays were within the range 0.6-14.4 × 105 cells/ml. Antibodies raised to heat-labile antigen were serotype-specific. Although they contain non-specific IgG fractions binding periplasmatic and cytosol proteins, the interactions of these immunoglobulins are not manifested under conditions of ELISA developed. |
| first_indexed | 2024-04-10T08:34:38Z |
| format | Article |
| id | doaj.art-36440221d7324ac58b0dbcc07b65b3e7 |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:34:38Z |
| publishDate | 2009-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-36440221d7324ac58b0dbcc07b65b3e72023-02-23T03:27:32ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172009-12-0127Special Issue 2SII66SII7410.17221/206/2009-CJFScjf-200911-0009Characterisation of antibodies for the immunochemical detection of Enterobacter sakazakiiIgor Hochel0Jiří Škvor1Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech RepublicDepartment of Medical Biophysics, 1 st Medical Faculty, Charles University in Prague, Prague, Czech RepublicAn indirect competitive enzyme immunoassay of Enterobacter sakazakii has been developed. The rabbit polyclonal antibodies to heat-labile or heat-stable antigen of the type strain E. sakazakii CNCTC 5739T were prepared for these purposes. The detection limits of enzyme immunoassays were within the range 0.6-14.4 × 105 cells/ml. Antibodies raised to heat-labile antigen were serotype-specific. Although they contain non-specific IgG fractions binding periplasmatic and cytosol proteins, the interactions of these immunoglobulins are not manifested under conditions of ELISA developed.https://cjfs.agriculturejournals.cz/artkey/cjf-200911-0009_characterisation-of-antibodies-for-the-immunochemical-detection-of-enterobacter-sakazakii.phpenterobacter sakazakiiheat-labile antigeniggelisacross-reactivity |
| spellingShingle | Igor Hochel Jiří Škvor Characterisation of antibodies for the immunochemical detection of Enterobacter sakazakii Czech Journal of Food Sciences enterobacter sakazakii heat-labile antigen igg elisa cross-reactivity |
| title | Characterisation of antibodies for the immunochemical detection of Enterobacter sakazakii |
| title_full | Characterisation of antibodies for the immunochemical detection of Enterobacter sakazakii |
| title_fullStr | Characterisation of antibodies for the immunochemical detection of Enterobacter sakazakii |
| title_full_unstemmed | Characterisation of antibodies for the immunochemical detection of Enterobacter sakazakii |
| title_short | Characterisation of antibodies for the immunochemical detection of Enterobacter sakazakii |
| title_sort | characterisation of antibodies for the immunochemical detection of enterobacter sakazakii |
| topic | enterobacter sakazakii heat-labile antigen igg elisa cross-reactivity |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-200911-0009_characterisation-of-antibodies-for-the-immunochemical-detection-of-enterobacter-sakazakii.php |
| work_keys_str_mv | AT igorhochel characterisationofantibodiesfortheimmunochemicaldetectionofenterobactersakazakii AT jiriskvor characterisationofantibodiesfortheimmunochemicaldetectionofenterobactersakazakii |