CD8αα+T cells exert a pro‐inflammatory role in patients with psoriasis
Abstract Background Psoriasis is a common chronic inflammatory disease caused by excessive activation of CD4+T cells, including Th17, Th1 and Th22. The role of CD8+T cells in psoriasis pathogenesis remains poorly understood. Aim To identify the phenotype of CD8+T cells in patients with psoriasis and...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-12-01
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| Series: | Skin Health and Disease |
| Online Access: | https://doi.org/10.1002/ski2.64 |
| _version_ | 1811207406321926144 |
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| author | Y. Y. Zhang Y. T. Lin L. Wang X. W. Sun E. L. Dang K. Xue W. G. Zhang K. M. Zhang G. Wang B. Li |
| author_facet | Y. Y. Zhang Y. T. Lin L. Wang X. W. Sun E. L. Dang K. Xue W. G. Zhang K. M. Zhang G. Wang B. Li |
| author_sort | Y. Y. Zhang |
| collection | DOAJ |
| description | Abstract Background Psoriasis is a common chronic inflammatory disease caused by excessive activation of CD4+T cells, including Th17, Th1 and Th22. The role of CD8+T cells in psoriasis pathogenesis remains poorly understood. Aim To identify the phenotype of CD8+T cells in patients with psoriasis and to investigate its role in the formation of lesions. Methods The phenotype of CD8+T cells in psoriatic lesions was detected by immunofluorescence staining. Flow cytometry was performed to detect their phenotype in peripheral blood. Thereafter, coculture of CD8αα+T cells with autogenous CD4+T cells was performed to investigate the function of CD8αα+T cells in patients with psoriasis. Finally, pro‐inflammatory factors produced by CD8αα+T cells were examined by immunofluorescence staining and flow cytometry. Results Compared to the CD8αβ+T cells, CD8αα+T cell infiltration in psoriatic lesions markedly increased. Moreover, epidermal CD8αα+T cells exhibited tissue‐resident memory T cells (TRM) phenotypes and dermal CD8αα+T cells exhibited effector memory (TEM) phenotypes in psoriatic lesions. Additionally, we found that CD8αα+T cells from patients with psoriasis did not express the markers of regulatory T cells and could promote the proliferation of CD4+T effector cells and produce interleukin‐17 and interferon‐γ. Conclusions Our findings demonstrate that CD8αα+T cells contribute to the pathogenesis of psoriasis by producing pro‐inflammatory factors. |
| first_indexed | 2024-04-12T04:02:29Z |
| format | Article |
| id | doaj.art-36451d0c06cf4234a85a3f46832ae74f |
| institution | Directory Open Access Journal |
| issn | 2690-442X |
| language | English |
| last_indexed | 2024-04-12T04:02:29Z |
| publishDate | 2021-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Skin Health and Disease |
| spelling | doaj.art-36451d0c06cf4234a85a3f46832ae74f2022-12-22T03:48:40ZengWileySkin Health and Disease2690-442X2021-12-0114n/an/a10.1002/ski2.64CD8αα+T cells exert a pro‐inflammatory role in patients with psoriasisY. Y. Zhang0Y. T. Lin1L. Wang2X. W. Sun3E. L. Dang4K. Xue5W. G. Zhang6K. M. Zhang7G. Wang8B. Li9Department of Dermatology Xijing Hospital Fourth Military Medical University Xi'an ChinaDepartment of Dermatology Xijing Hospital Fourth Military Medical University Xi'an ChinaDepartment of Dermatology Xijing Hospital Fourth Military Medical University Xi'an ChinaDepartment of Dermatology Xijing Hospital Fourth Military Medical University Xi'an ChinaDepartment of Dermatology Xijing Hospital Fourth Military Medical University Xi'an ChinaDepartment of Dermatology Xijing Hospital Fourth Military Medical University Xi'an ChinaDepartment of Dermatology Xijing Hospital Fourth Military Medical University Xi'an ChinaInstitute of Dermatology Taiyuan City Central Hospital Shanxi Key Laboratory for Immunological Dermatosis Taiyuan ChinaDepartment of Dermatology Xijing Hospital Fourth Military Medical University Xi'an ChinaDepartment of Dermatology Xijing Hospital Fourth Military Medical University Xi'an ChinaAbstract Background Psoriasis is a common chronic inflammatory disease caused by excessive activation of CD4+T cells, including Th17, Th1 and Th22. The role of CD8+T cells in psoriasis pathogenesis remains poorly understood. Aim To identify the phenotype of CD8+T cells in patients with psoriasis and to investigate its role in the formation of lesions. Methods The phenotype of CD8+T cells in psoriatic lesions was detected by immunofluorescence staining. Flow cytometry was performed to detect their phenotype in peripheral blood. Thereafter, coculture of CD8αα+T cells with autogenous CD4+T cells was performed to investigate the function of CD8αα+T cells in patients with psoriasis. Finally, pro‐inflammatory factors produced by CD8αα+T cells were examined by immunofluorescence staining and flow cytometry. Results Compared to the CD8αβ+T cells, CD8αα+T cell infiltration in psoriatic lesions markedly increased. Moreover, epidermal CD8αα+T cells exhibited tissue‐resident memory T cells (TRM) phenotypes and dermal CD8αα+T cells exhibited effector memory (TEM) phenotypes in psoriatic lesions. Additionally, we found that CD8αα+T cells from patients with psoriasis did not express the markers of regulatory T cells and could promote the proliferation of CD4+T effector cells and produce interleukin‐17 and interferon‐γ. Conclusions Our findings demonstrate that CD8αα+T cells contribute to the pathogenesis of psoriasis by producing pro‐inflammatory factors.https://doi.org/10.1002/ski2.64 |
| spellingShingle | Y. Y. Zhang Y. T. Lin L. Wang X. W. Sun E. L. Dang K. Xue W. G. Zhang K. M. Zhang G. Wang B. Li CD8αα+T cells exert a pro‐inflammatory role in patients with psoriasis Skin Health and Disease |
| title | CD8αα+T cells exert a pro‐inflammatory role in patients with psoriasis |
| title_full | CD8αα+T cells exert a pro‐inflammatory role in patients with psoriasis |
| title_fullStr | CD8αα+T cells exert a pro‐inflammatory role in patients with psoriasis |
| title_full_unstemmed | CD8αα+T cells exert a pro‐inflammatory role in patients with psoriasis |
| title_short | CD8αα+T cells exert a pro‐inflammatory role in patients with psoriasis |
| title_sort | cd8αα t cells exert a pro inflammatory role in patients with psoriasis |
| url | https://doi.org/10.1002/ski2.64 |
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