Osteopontin Gene Polymorphism and Urinary OPN Excretion in Patients with Immunoglobulin A Nephropathy
Osteopontin (OPN) is a glycoprotein involved in the pathogenesis of multiple autoimmune and inflammatory conditions. However, the association of variants of secreted phosphoprotein 1 gene (<i>SPP1</i>), which encodes OPN, with immunoglobulin A nephropathy (IgAN) has not been examined up...
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MDPI AG
2019-05-01
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| author | Beata Kaleta Natalia Krata Radosław Zagożdżon Krzysztof Mucha |
| author_facet | Beata Kaleta Natalia Krata Radosław Zagożdżon Krzysztof Mucha |
| author_sort | Beata Kaleta |
| collection | DOAJ |
| description | Osteopontin (OPN) is a glycoprotein involved in the pathogenesis of multiple autoimmune and inflammatory conditions. However, the association of variants of secreted phosphoprotein 1 gene (<i>SPP1</i>), which encodes OPN, with immunoglobulin A nephropathy (IgAN) has not been examined up to date. Moreover, the role of OPN in disease pathogenesis and clinical manifestations is not fully known. Therefore, the aim of the study was to determine the frequency of four single nucleotide polymorphisms (SNiPs) of <i>SPP1</i> gene, as well as the urinary OPN excretion in IgAN patients and healthy controls. In total, 58 Caucasian patients with biopsy-proven IgAN and 184 gender-, age-, and ethnically-matched healthy controls were genotyped for rs1126616, rs1126772, rs9138, and rs7687316/rs3841116 polymorphisms by real time polymerase chain reaction (RT-PCR). Urinary OPN concentration was determined by enzyme-linked immunosorbent assay (ELISA) in 58 IgAN patients and 19 controls. <i>SPP1</i> SNiPs, as well as urinary OPN excretion, were analyzed in relation to their possible associations with the clinicopathological parameters. The frequency of the minor TT/CT genotypes of rs1126616 was significantly higher in IgAN patients compared to controls (P = 0.0217). Similarly, the minor (CC/AC) genotypes and the C allele of rs9138 were more frequent in IgAN patients (P = 0.0425 and P = 0.0112, respectively). Moreover, these two SNiPs were associated with the higher urinary OPN excretion (P < 0.05). These findings suggest that rs1126616, as well as rs9138, may be associated with IgAN development, however future studies in this field are required. |
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| spelling | doaj.art-3646950a7a9541ab9c210afe6a0fbc5a2023-09-02T11:42:47ZengMDPI AGCells2073-44092019-05-018652410.3390/cells8060524cells8060524Osteopontin Gene Polymorphism and Urinary OPN Excretion in Patients with Immunoglobulin A NephropathyBeata Kaleta0Natalia Krata1Radosław Zagożdżon2Krzysztof Mucha3Department of Clinical Immunology, Medical University of Warsaw, Nowogrodzka 59, 02-006 Warsaw, PolandDepartment of Immunology, Transplantology and Internal Diseases, Medical University of Warsaw, Nowogrodzka 59, 02-006 Warsaw, PolandDepartment of Clinical Immunology, Medical University of Warsaw, Nowogrodzka 59, 02-006 Warsaw, PolandDepartment of Immunology, Transplantology and Internal Diseases, Medical University of Warsaw, Nowogrodzka 59, 02-006 Warsaw, PolandOsteopontin (OPN) is a glycoprotein involved in the pathogenesis of multiple autoimmune and inflammatory conditions. However, the association of variants of secreted phosphoprotein 1 gene (<i>SPP1</i>), which encodes OPN, with immunoglobulin A nephropathy (IgAN) has not been examined up to date. Moreover, the role of OPN in disease pathogenesis and clinical manifestations is not fully known. Therefore, the aim of the study was to determine the frequency of four single nucleotide polymorphisms (SNiPs) of <i>SPP1</i> gene, as well as the urinary OPN excretion in IgAN patients and healthy controls. In total, 58 Caucasian patients with biopsy-proven IgAN and 184 gender-, age-, and ethnically-matched healthy controls were genotyped for rs1126616, rs1126772, rs9138, and rs7687316/rs3841116 polymorphisms by real time polymerase chain reaction (RT-PCR). Urinary OPN concentration was determined by enzyme-linked immunosorbent assay (ELISA) in 58 IgAN patients and 19 controls. <i>SPP1</i> SNiPs, as well as urinary OPN excretion, were analyzed in relation to their possible associations with the clinicopathological parameters. The frequency of the minor TT/CT genotypes of rs1126616 was significantly higher in IgAN patients compared to controls (P = 0.0217). Similarly, the minor (CC/AC) genotypes and the C allele of rs9138 were more frequent in IgAN patients (P = 0.0425 and P = 0.0112, respectively). Moreover, these two SNiPs were associated with the higher urinary OPN excretion (P < 0.05). These findings suggest that rs1126616, as well as rs9138, may be associated with IgAN development, however future studies in this field are required.https://www.mdpi.com/2073-4409/8/6/524osteopontingenepolymorphism<i>SPP1</i>immunoglobulin A nephropathykidney |
| spellingShingle | Beata Kaleta Natalia Krata Radosław Zagożdżon Krzysztof Mucha Osteopontin Gene Polymorphism and Urinary OPN Excretion in Patients with Immunoglobulin A Nephropathy Cells osteopontin gene polymorphism <i>SPP1</i> immunoglobulin A nephropathy kidney |
| title | Osteopontin Gene Polymorphism and Urinary OPN Excretion in Patients with Immunoglobulin A Nephropathy |
| title_full | Osteopontin Gene Polymorphism and Urinary OPN Excretion in Patients with Immunoglobulin A Nephropathy |
| title_fullStr | Osteopontin Gene Polymorphism and Urinary OPN Excretion in Patients with Immunoglobulin A Nephropathy |
| title_full_unstemmed | Osteopontin Gene Polymorphism and Urinary OPN Excretion in Patients with Immunoglobulin A Nephropathy |
| title_short | Osteopontin Gene Polymorphism and Urinary OPN Excretion in Patients with Immunoglobulin A Nephropathy |
| title_sort | osteopontin gene polymorphism and urinary opn excretion in patients with immunoglobulin a nephropathy |
| topic | osteopontin gene polymorphism <i>SPP1</i> immunoglobulin A nephropathy kidney |
| url | https://www.mdpi.com/2073-4409/8/6/524 |
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