H2O2 selectively damages the binuclear iron-sulfur cluster N1b of respiratory complex I
Abstract NADH:ubiquinone oxidoreductase, respiratory complex I, plays a major role in cellular energy metabolism by coupling electron transfer with proton translocation. Electron transfer is catalyzed by a flavin mononucleotide and a series of iron-sulfur (Fe/S) clusters. As a by-product of the reac...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-34821-5 |
| _version_ | 1797827495059259392 |
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| author | Lisa Strotmann Caroline Harter Tatjana Gerasimova Kevin Ritter Henning J. Jessen Daniel Wohlwend Thorsten Friedrich |
| author_facet | Lisa Strotmann Caroline Harter Tatjana Gerasimova Kevin Ritter Henning J. Jessen Daniel Wohlwend Thorsten Friedrich |
| author_sort | Lisa Strotmann |
| collection | DOAJ |
| description | Abstract NADH:ubiquinone oxidoreductase, respiratory complex I, plays a major role in cellular energy metabolism by coupling electron transfer with proton translocation. Electron transfer is catalyzed by a flavin mononucleotide and a series of iron-sulfur (Fe/S) clusters. As a by-product of the reaction, the reduced flavin generates reactive oxygen species (ROS). It was suggested that the ROS generated by the respiratory chain in general could damage the Fe/S clusters of the complex. Here, we show that the binuclear Fe/S cluster N1b is specifically damaged by H2O2, however, only at high concentrations. But under the same conditions, the activity of the complex is hardly affected, since N1b can be easily bypassed during electron transfer. |
| first_indexed | 2024-04-09T12:49:14Z |
| format | Article |
| id | doaj.art-3647ff54d0fb47a6a4ba0d6b9b9dbcf2 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T12:49:14Z |
| publishDate | 2023-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-3647ff54d0fb47a6a4ba0d6b9b9dbcf22023-05-14T11:16:59ZengNature PortfolioScientific Reports2045-23222023-05-011311910.1038/s41598-023-34821-5H2O2 selectively damages the binuclear iron-sulfur cluster N1b of respiratory complex ILisa Strotmann0Caroline Harter1Tatjana Gerasimova2Kevin Ritter3Henning J. Jessen4Daniel Wohlwend5Thorsten Friedrich6Institut für Biochemie, Albert-Ludwigs-Universität FreiburgInstitut für Biochemie, Albert-Ludwigs-Universität FreiburgInstitut für Biochemie, Albert-Ludwigs-Universität FreiburgInstitut für Organische Chemie, Albert-Ludwigs-Universität FreiburgInstitut für Organische Chemie, Albert-Ludwigs-Universität FreiburgInstitut für Biochemie, Albert-Ludwigs-Universität FreiburgInstitut für Biochemie, Albert-Ludwigs-Universität FreiburgAbstract NADH:ubiquinone oxidoreductase, respiratory complex I, plays a major role in cellular energy metabolism by coupling electron transfer with proton translocation. Electron transfer is catalyzed by a flavin mononucleotide and a series of iron-sulfur (Fe/S) clusters. As a by-product of the reaction, the reduced flavin generates reactive oxygen species (ROS). It was suggested that the ROS generated by the respiratory chain in general could damage the Fe/S clusters of the complex. Here, we show that the binuclear Fe/S cluster N1b is specifically damaged by H2O2, however, only at high concentrations. But under the same conditions, the activity of the complex is hardly affected, since N1b can be easily bypassed during electron transfer.https://doi.org/10.1038/s41598-023-34821-5 |
| spellingShingle | Lisa Strotmann Caroline Harter Tatjana Gerasimova Kevin Ritter Henning J. Jessen Daniel Wohlwend Thorsten Friedrich H2O2 selectively damages the binuclear iron-sulfur cluster N1b of respiratory complex I Scientific Reports |
| title | H2O2 selectively damages the binuclear iron-sulfur cluster N1b of respiratory complex I |
| title_full | H2O2 selectively damages the binuclear iron-sulfur cluster N1b of respiratory complex I |
| title_fullStr | H2O2 selectively damages the binuclear iron-sulfur cluster N1b of respiratory complex I |
| title_full_unstemmed | H2O2 selectively damages the binuclear iron-sulfur cluster N1b of respiratory complex I |
| title_short | H2O2 selectively damages the binuclear iron-sulfur cluster N1b of respiratory complex I |
| title_sort | h2o2 selectively damages the binuclear iron sulfur cluster n1b of respiratory complex i |
| url | https://doi.org/10.1038/s41598-023-34821-5 |
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