MicroRNA and Hemophilia-A Disease: Bioinformatics Prediction and Experimental Analysis
Objective: Hemophilia-A is a common genetic abnormality resulted from decreased or lack of factor VIII (FVIII) pro-coagulant protein function caused by mutations in the F8 gene. Majority of molecular studies consider screening of mutations and their relevant impacts on the quality and expression l...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2021-08-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | https://celljournal.org/journal/article/fulltext/micrornas-and-hemophilia-a-disease-bioinformatics-prediction-and-experimental-analysis.pdf |
| _version_ | 1819087026871336960 |
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| author | Halimeh Rezaei Majid Motovali-Bashi Seyed Javad Mowla |
| author_facet | Halimeh Rezaei Majid Motovali-Bashi Seyed Javad Mowla |
| author_sort | Halimeh Rezaei |
| collection | DOAJ |
| description | Objective: Hemophilia-A is a common genetic abnormality resulted from decreased or lack of factor VIII (FVIII) pro-coagulant
protein function caused by mutations in the F8 gene. Majority of molecular studies consider screening of mutations and their
relevant impacts on the quality and expression levels of FVIII. Interestingly, some of the functions in FVIII suggest a probable
involvement of small non-coding RNAs embedded within the sequence of F8 gene. Therefore, microRNAs which are encoded
within the F8 gene might have a role in hemophilia development. In this study, miRNAs production in the F8 gene was investigated
by bioinformatics prediction and experimental validation.
Materials and Methods: In this experimental study, bioinformatics tools have been utilized to seek the novel microRNAs
inserted within human F8 gene. The ability to express new microRNAs in F8 locus was studied through reliable bioinformatics
databases such as SSCProfiler, RNA fold, miREval, miR-FIND, UCSC genome browser and miRBase. Then, expression and
processing of the predicted microRNAs were examined based on bioinformatics methods, in the HEK293 cell lines.
Results: We are unable to confirm existence of the considered mature microRNAs in the transfected cells.
Conclusion: We hope that through changing experimental conditions, designing new primers or altering cell lines as well as the
expression of vectors, exogenous and endogenous expressions of the predicted miRNA will be confirmed. |
| first_indexed | 2024-12-21T21:29:36Z |
| format | Article |
| id | doaj.art-3649018d25124f7bb50301ddde63dc52 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-12-21T21:29:36Z |
| publishDate | 2021-08-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-3649018d25124f7bb50301ddde63dc522022-12-21T18:49:39ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142021-08-0123334134810.22074/cellj.2021.7109MicroRNA and Hemophilia-A Disease: Bioinformatics Prediction and Experimental AnalysisHalimeh Rezaei0Majid Motovali-Bashi1Seyed Javad Mowla2Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Sciences and Technology, University of Isfahan, Isfahan, IranDepartment of Cell and Molecular Biology and Microbiology, Faculty of Biological Sciences and Technology, University of Isfahan, Isfahan, IranMolecular Genetics Department, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, IranObjective: Hemophilia-A is a common genetic abnormality resulted from decreased or lack of factor VIII (FVIII) pro-coagulant protein function caused by mutations in the F8 gene. Majority of molecular studies consider screening of mutations and their relevant impacts on the quality and expression levels of FVIII. Interestingly, some of the functions in FVIII suggest a probable involvement of small non-coding RNAs embedded within the sequence of F8 gene. Therefore, microRNAs which are encoded within the F8 gene might have a role in hemophilia development. In this study, miRNAs production in the F8 gene was investigated by bioinformatics prediction and experimental validation. Materials and Methods: In this experimental study, bioinformatics tools have been utilized to seek the novel microRNAs inserted within human F8 gene. The ability to express new microRNAs in F8 locus was studied through reliable bioinformatics databases such as SSCProfiler, RNA fold, miREval, miR-FIND, UCSC genome browser and miRBase. Then, expression and processing of the predicted microRNAs were examined based on bioinformatics methods, in the HEK293 cell lines. Results: We are unable to confirm existence of the considered mature microRNAs in the transfected cells. Conclusion: We hope that through changing experimental conditions, designing new primers or altering cell lines as well as the expression of vectors, exogenous and endogenous expressions of the predicted miRNA will be confirmed.https://celljournal.org/journal/article/fulltext/micrornas-and-hemophilia-a-disease-bioinformatics-prediction-and-experimental-analysis.pdfbioinformaticsfactor viiihek293 cellshemophilia-amicrornas |
| spellingShingle | Halimeh Rezaei Majid Motovali-Bashi Seyed Javad Mowla MicroRNA and Hemophilia-A Disease: Bioinformatics Prediction and Experimental Analysis Cell Journal bioinformatics factor viii hek293 cells hemophilia-a micrornas |
| title | MicroRNA and Hemophilia-A Disease: Bioinformatics Prediction and Experimental Analysis |
| title_full | MicroRNA and Hemophilia-A Disease: Bioinformatics Prediction and Experimental Analysis |
| title_fullStr | MicroRNA and Hemophilia-A Disease: Bioinformatics Prediction and Experimental Analysis |
| title_full_unstemmed | MicroRNA and Hemophilia-A Disease: Bioinformatics Prediction and Experimental Analysis |
| title_short | MicroRNA and Hemophilia-A Disease: Bioinformatics Prediction and Experimental Analysis |
| title_sort | microrna and hemophilia a disease bioinformatics prediction and experimental analysis |
| topic | bioinformatics factor viii hek293 cells hemophilia-a micrornas |
| url | https://celljournal.org/journal/article/fulltext/micrornas-and-hemophilia-a-disease-bioinformatics-prediction-and-experimental-analysis.pdf |
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